Genetic control and expression of the major ejaculatory bulb protein (PEB-me) inDrosophila melanogaster

Ludwig, Michael; I, Uspensky; Ivanov, A. I.; Kopantseva, M. R.; Dianov, Christem M.; Tamarina, Natalia A.; Korochkin, L. I.

doi:10.1007/bf00590103

Cited by 34 publications

(28 citation statements)

References 33 publications

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“…To determine the role of the PMP in D. melanogaster reproduction, we targeted the major EB protein PEBme (Ludwig et al 1991;Lung and Wolfner 2001). Numerous male reproductive tract GAL4 drivers were available, but none drove specific expression in the EB.…”

Section: Resultsmentioning

confidence: 99%

“…To date, only three D. melanogaster MP proteins have been identified: the PMP proteins PEBme and PEBII (Ludwig et al 1991;Lung and Wolfner 2001;Bretman et al 2010) and the AMP protein Acp36DE (Bertram et al 1996). Of these, Acp36DE ( Table S1.…”

Section: Identification Of Additional Mp Proteinsmentioning

confidence: 99%

“…Two PMP proteins have been identified previously: protein of the ejaculatory bulb of melanogaster (PEBme) (Ludwig et al 1991;Lung and Wolfner 2001) and protein of the ejaculatory bulb II (PEBII) (Bretman et al 2010). While the function of PEBme is unknown, PEBII reduces the likelihood of female remating in the first 4 hr postmating (Bretman et al 2010).…”

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Retention of Ejaculate by Drosophila melanogaster Females Requires the Male-Derived Mating Plug Protein PEBme

et al. 2015

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Within the mated reproductive tracts of females of many taxa, seminal fluid proteins (SFPs) coagulate into a structure known as the mating plug (MP). MPs have diverse roles, including preventing female remating, altering female receptivity postmating, and being necessary for mated females to successfully store sperm. The Drosophila melanogaster MP, which is maintained in the mated female for several hours postmating, is comprised of a posterior MP (PMP) that forms quickly after mating begins and an anterior MP (AMP) that forms later. The PMP is composed of seminal proteins from the ejaculatory bulb (EB) of the male reproductive tract. To examine the role of the PMP protein PEBme in D. melanogaster reproduction, we identified an EB GAL4 driver and used it to target PEBme for RNA interference (RNAi) knockdown. PEBme knockdown in males compromised PMP coagulation in their mates and resulted in a significant reduction in female fertility, adversely affecting postmating uterine conformation, sperm storage, mating refractoriness, egg laying, and progeny generation. These defects resulted from the inability of females to retain the ejaculate in their reproductive tracts after mating. The uncoagulated MP impaired uncoupling by the knockdown male, and when he ultimately uncoupled, the ejaculate was often pulled out of the female. Thus, PEBme and MP coagulation are required for optimal fertility in D. melanogaster. Given the importance of the PMP for fertility, we identified additional MP proteins by mass spectrometry and found fertility functions for two of them. Our results highlight the importance of the MP and the proteins that comprise it in reproduction and suggest that in Drosophila the PMP is required to retain the ejaculate within the female reproductive tract, ensuring the storage of sperm by mated females.KEYWORDS mating plug; sperm storage; PEBme; Drosophila reproduction I N numerous species comprising diverse taxa, a solidified structure forms inside the female reproductive tract during (or shortly after) mating that is referred to as the mating plug (MP; also called the copulatory plug; we will refer to these structures collectively as MPs). MPs are largely a coagulation of male seminal fluid components. In species that produce a MP, its role in reproduction varies. In some species, MP formation is thought to guard against sperm competition. For example, in primates, MPs are seen most often in species whose females mate multiply (Dixson and Anderson 2002). Primate MPs have been suggested to prevent remating (Dorus et al. 2004), thus acting as a form of passive mate guarding (Dunham and Rudolf 2009). In the mouse, perturbing (Murer et al. 2001) or preventing (Dean 2013) MP formation reduces male fertility; in the absence of MP formation, sperm migration to the sites of fertilization is impaired (Dean 2013) receptivity in the short term (Polak et al. 1998;Bretman et al. 2010). In bumblebees, MPs physically switch off receptivity Sauter et al. 2001) and have functions related to sperm competition (Duvo...

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Section: Resultsmentioning

confidence: 99%

Section: Identification Of Additional Mp Proteinsmentioning

confidence: 99%

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Retention of Ejaculate by Drosophila melanogaster Females Requires the Male-Derived Mating Plug Protein PEBme

et al. 2015

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“…The refractory period to remating in females takes several hours to develop 79 and, at least in laboratory settings, females can remate until this effect takes place 78 The mating plug reduces remating rates in the first 4 h after mating 78 . PEBII and PEB-me, two SFPs made in the ejaculatory bulb, are required for the formation of this short-term barrier to reinsemination 23 , 24 , 78 , 80 . The mating plug persists in the female reproductive tract until it is ejected by the female 21 , 36 …”

Section: Male Influences On Sperm Recruitment Viability and Usagementioning

confidence: 99%

Oh, the places they’ll go

2012

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Among most animals with internal fertilization, females store sperm in specific regions of their reproductive tract for later use. Sperm storage enables prolonged fertility, physical and temporal separation of mating from fertilization and, when females mate with multiple males, opportunities for differential use of the various males’ sperm. Thus, stored sperm move within the female reproductive tract as well as to several potential fates – fertilization, displacement by other sperm or ejection by the female. Drosophila melanogaster is a leading model system for elucidating both the mechanisms and evolutionary consequences of female sperm storage and differential male fertilization success. The prominence of Drosophila is due, in part, to the ability to examine processes influencing sperm movement and fate at several biological levels, from molecules to organ systems. In this review, we describe male and female factors, as well as their interactions, involved in female sperm storage and differential male fertilization success.

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“…[Males have also been reported to donate cuticular hydrocarbons, and elemental phosphorus, to their mates (for reviews, see Antony and Jallon, 1982;Jallon, 1984; also see Markow et al, 2001). Consideration of these, and of the contributions of the male's ejaculatory duct and bulb (eg Gilbert et al, 1981;Cavener and MacIntyre, 1983;Ludwig et al, 1991;Lung et al, 2001b) is beyond the scope of this review.] Sperm and seminal fluid proteins both exert postmating influences on female insects.…”

Section: Introductionmentioning

confidence: 99%

The gifts that keep on giving: physiological functions and evolutionary dynamics of male seminal proteins in Drosophila

2002

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During mating, males transfer seminal proteins and peptides, along with sperm, to their mates. In Drosophila melanogaster, seminal proteins made in the male's accessory gland stimulate females' egg production and ovulation, reduce their receptivity to mating, mediate sperm storage, cause part of the survival cost of mating to females, and may protect reproductive tracts or gametes from microbial attack. The physiological functions of these proteins indicate that males provide their mates with molecules that initiate important reproductive responses in females. A new comprehensive EST screen, in conjunction with earlier screens, has identified ෂ90% of the predicted secreted accessory gland proteins (Acps). Most Acps are novel proteins and many appear to be secreted peptides or prohormones. Acps also include modification enzymes such as proteases and

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Genetic control and expression of the major ejaculatory bulb protein (PEB-me) inDrosophila melanogaster

Cited by 34 publications

References 33 publications

Retention of Ejaculate by Drosophila melanogaster Females Requires the Male-Derived Mating Plug Protein PEBme

Retention of Ejaculate by Drosophila melanogaster Females Requires the Male-Derived Mating Plug Protein PEBme

Oh, the places they’ll go

The gifts that keep on giving: physiological functions and evolutionary dynamics of male seminal proteins in Drosophila

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