Genetic control and transgressive segregation of zinc, iron, potassium, phosphorus, calcium, and sodium accumulation in cowpea (Vigna unguiculata) seeds

Santos, C. A. F.; Boiteux, L. S.

doi:10.4238/2015.january.16.10

Cited by 17 publications

(13 citation statements)

References 15 publications

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“…In earlier studies, with other Vigna species like mungbean, an average value of 30.0 and 40.0 mg kg –1 of seed Fe have been reported ( Wu et al, 2020 ), which confirmed that V. stipulacea is the potential source for mitigating Fe deficiency of rural folks especially adolescent children and female with acute problems of anemia. Similarly, the present finding regarding grain Fe concentration was corroborated with the wider variation reported in the case of other small-seeded legumes like lentil ( Thavarajah et al, 2017 ; Kumar et al, 2018 ; Shrestha et al, 2018 ); common bean ( de Araújo et al, 2003 ; Blair et al, 2009 ) and cowpea ( Boukar et al, 2011 ; Santos and Boiteux, 2015 ). On the contrary, a narrow range of variation (20.6–71.0 mg kg –1 ) was reported regarding grain Zn concentration compared to grain Fe in our study.…”

Section: Discussionsupporting

confidence: 90%

Understanding G × E Interaction for Nutritional and Antinutritional Factors in a Diverse Panel of Vigna stipulacea (Lam.) Kuntz Germplasm Tested Over the Locations

et al. 2021

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Micronutrient malnutrition or hidden hunger is a serious challenge toward societal well-being. Vigna stipulacea (Lam.) Kuntz (known locally as Minni payaru), is an underutilized legume that has the potential to be a global food legume due to its rich nutrient profile. In the present study, 99 accessions of V. stipulacea were tested for iron (Fe), zinc (Zn), calcium (Ca), protein, and phytate concentrations over two locations for appraisal of stable nutrient-rich sources. Analysis of variance revealed significant effects of genotype for all the traits over both locations. Fe concentration ranged from 29.35–130.96 mg kg–1 whereas Zn concentration ranged from 19.44 to 74.20 mg kg–1 across both locations. The highest grain Ca concentration was 251.50 mg kg–1 whereas the highest grain protein concentration was recorded as 25.73%. In the case of grain phytate concentration, a genotype with the lowest value is desirable. IC622867 (G-99) was the lowest phytate containing accession at both locations. All the studied traits revealed highly significant genotypic variances and highly significant genotype × location interaction though less in magnitude than the genotypic variance. GGE Biplot analysis detected that, for grain Fe, Zn, and Ca concentration the ‘ideal’ genotypes were IC331457 (G-75), IC331610 (G-76), and IC553564 (G-60), respectively, whereas for grain protein concentration IC553521 (G-27) was the most “ideal type.” For phytate concentration, IC351407 (G-95) and IC550523 (G-99) were considered as ‘ideal’ and ‘desirable,’ respectively. Based on the desirability index, Location 1 (Kanpur) was identified as ideal for Fe, Zn, Ca, and phytate, and for grain protein concentration, Location 2 (New Delhi) was the ideal type. A significant positive correlation was detected between grain Fe as well as grain Zn and protein concentration considering the pooled analysis over both the locations where as a significant negative association was observed between phytate and protein concentration over the locations. This study has identified useful donors and enhanced our knowledge toward the development of biofortified Vigna cultivars. Promoting domestication of this nutrient-rich semi-domesticated, underutilized species will boost sustainable agriculture and will contribute toward alleviating hidden hunger.

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Section: Discussionsupporting

confidence: 90%

Understanding G × E Interaction for Nutritional and Antinutritional Factors in a Diverse Panel of Vigna stipulacea (Lam.) Kuntz Germplasm Tested Over the Locations

et al. 2021

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“…This success has paved the way for advances in other pulses. Studies on Fe accumulation traits have been performed on cowpea (Fernandes Santos and Boiteux, 2015 ), chickpea (Diapari et al, 2014 ), pea and lentil (Ray et al, 2014 ), and test trials are currently underway for some of them (HarvestPlus, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Investigation of Baseline Iron Levels in Australian Chickpea and Evaluation of a Transgenic Biofortification Approach

et al. 2018

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Iron deficiency currently affects over two billion people worldwide despite significant advances in technology and society aimed at mitigating this global health problem. Biofortification of food staples with iron (Fe) represents a sustainable approach for alleviating human Fe deficiency in developing countries, however, biofortification efforts have focused extensively on cereal staples while pulses have been largely overlooked. In this study we describe a genetic engineering (GE) approach to biofortify the pulse crop, chickpea (Cicer arietinum L.), with Fe using a combination of the chickpea nicotianamine synthase 2 (CaNAS2) and soybean (Glycine max) ferritin (GmFER) genes which function in Fe transport and storage, respectively. This study consists of three main components: (1) the establishment for baseline Fe concentration of existing germplam, (2) the isolation and study of expression pattern of the novel CaNAS2 gene, and (3) the generation of GE chickpea overexpressing the CaNAS2 and GmFER genes. Seed of six commercial chickpea cultivars was collected from four different field locations in Australia and assessed for seed Fe concentration. The results revealed little difference between the cultivars assessed, and that chickpea seed Fe was negatively affected where soil Fe bioavailability is low. The desi cultivar HatTrick was then selected for further study. From it, the CaNAS2 gene was cloned and its expression in different tissues examined. The gene was found to be expressed in multiple vegetative tissues under Fe-sufficient conditions, suggesting that it may play a housekeeping role in systemic translocation of Fe. Two GE chickpea events were then generated and the overexpression of the CaNAS2 and GmFER transgenes confirmed. Analysis of nicotianamine (NA) and Fe levels in the GE seeds revealed that NA was nearly doubled compared to the null control while Fe concentration was not changed. Increased NA content in chickpea seed is likely to translate into increased Fe bioavailability and may thus overcome the effect of the bioavailability inhibitors found in pulses; however, further study is required to confirm this. This is the first known example of GE Fe biofortified chickpea; information gleaned from this study can feed into future pulse biofortification work to help alleviate global Fe deficiency.

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“…The additive effects indicated that the alleles for higher element concentrations were contributed by Ziguangmangluoerling, and that these may be the most important components for enhancing element concentrations through methods of genealogical improvement. Significant additive genetic effects as well as parental mean effects have previously been detected for P, K, Ca, and Na (Fernandes Santos and Boiteux, 2015). Co-localization of QTLs for element concentration has previously been found in various species, such as rice (Yu et al, 2015) and in maize grain (Simić et al, 2012).…”

Section: Qtl Detection and Pleiotropy For Eight Element Concentrationmentioning

confidence: 88%

Identification of quantitative trait loci for mineral elements in grains and grass powder of barley

Zeng¹,

Du²,

Yang³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Mineral elements in barley (Hordeum vulgare) play an important physiological role in global human health. In this study, quantitative trait loci (QTLs) for concentration of nine mineral elements in barley grain and grass powder were detected in a population of 193 recombinant inbred lines of the barley cross Ziguangmangluoerling x Schooner and the parents. We observed large genetic variation contributing to element concentrations in both grains and grass powder. The mean K, Ca, and Fe concentrations in grass powder were 6.67, 12.00, and 4.58 times that of regenerating barley grains. In grains, 17 2 Y.W. Zeng et al. Genetics and Molecular Research 15 (4): gmr15049103QTLs that accounted for 6.36-64.08% of the phenotypic variation in Zn, Mg, Ca, K, Na, Mn, Fe, and P concentrations were identified. In grass powder, seven QTLs were identified; these accounted for 6.03-21.86% of the variation in Ca, Zn, Mg, K, Fe, and Cu concentrations. These QTLs affecting elements in grain and grass powder are so far unreported in barley. To our knowledge, QTLs with pleiotropic effects for three elements were also identified for the first time in barley. The qK1/qMg1/qCa1 region between markers Bmag0211 and GBMS0014 on chromosome 1H was shown to have large additive effects for Mg, Ca, and K concentrations in grains. These additive effects indicated that the high element (Mg, Ca, Zn, Mn, and K) alleles were contributed by Ziguangmangluoerling. These results will further our understanding of the genetic basis of mineral elements and help us develop markers linked with mineral elements for marker-assisted selection breeding in barley.

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Genetic control and transgressive segregation of zinc, iron, potassium, phosphorus, calcium, and sodium accumulation in cowpea (Vigna unguiculata) seeds

Abstract: ABSTRACT. Cowpea crop, through combining a range of essential minerals with high quality proteins, plays an important role in providing nutritional security to human population living in semi-arid regions.

Cited by 17 publications

References 15 publications

Understanding G × E Interaction for Nutritional and Antinutritional Factors in a Diverse Panel of Vigna stipulacea (Lam.) Kuntz Germplasm Tested Over the Locations

Understanding G × E Interaction for Nutritional and Antinutritional Factors in a Diverse Panel of Vigna stipulacea (Lam.) Kuntz Germplasm Tested Over the Locations

Investigation of Baseline Iron Levels in Australian Chickpea and Evaluation of a Transgenic Biofortification Approach

Identification of quantitative trait loci for mineral elements in grains and grass powder of barley

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