Genetic control of<i>Aedes</i>mosquitoes

Alphey, Luke; McKemey, Andrew R.; Nimmo, Derric; Neira, Marco; Lacroix, Renaud; Matzen, Kelly; Beech, Camilla

doi:10.1179/2047773213y.0000000095

Cited by 134 publications

(93 citation statements)

References 112 publications

(105 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genetic methods for suppressing vector mosquito populations have long been discussed as an alternative op-tion to the existing control strategies given their potential advantages, including effectiveness and species specificity (Alphey & Collins, 2002;Alphey et al, 2013). Because of the evolution of mosquitos, site-specific gene editing is urgently needed to address strains resistant to human pathogens and to suppress mosquito populations by certain genetic elements.…”

Section: Discussionmentioning

confidence: 99%

Construction of an efficient genomic editing system with CRISPR/Cas9 in the vector mosquito Aedes albopictus

et al. 2018

View full text Add to dashboard Cite

Aedes (Stegomyia) albopictus, also known as the Asian tiger mosquito, is a mosquito which originated in Asia. In recent years, it has become increasingly rampant throughout the world. This mosquito can transmit several arboviruses, including dengue, Zika and chikungunya viruses, and is considered a public health threat. Despite the urgent need of genome engineering to analyze specific gene functions, progress in genetical manipulation of Ae. albopictus has been slow due to a lack of efficient methods and genetic markers. In the present study, we established targeted disruptions in two genes, kynurenine hydroxylase (kh) and dopachrome conversion enzyme (yellow), to analyze the feasibility of generating visible phenotypes with genome editing by the clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR‐associated protein 9 (Cas9) system in Ae. albopictus. Following Cas9 single guide RNA ribonucleoprotein injection into the posterior end of pre‐blastoderm embryos, 30%–50% of fertile survivors produced alleles that failed to complement existing kh and yellow mutations. Complete eye and body pigmentation defects were readily observed in G1 pupae and adults, indicating successful generation of highly heritable mutations. We conclude that the CRISPR/Cas9‐mediated gene editing system can be used in Ae. albopictus and that it can be adopted as an efficient tool for genome‐scale analysis and biological study.

show abstract

Section: Discussionmentioning

confidence: 99%

Construction of an efficient genomic editing system with CRISPR/Cas9 in the vector mosquito Aedes albopictus

et al. 2018

View full text Add to dashboard Cite

show abstract

“…albopictus populations (Fig. 3) (Ferreira et al, 2008;Alphey et al, 2010;Walker et al, 2011;Boyer, 2012;Alphey et al, 2013;Massonnet-Bruneel et al, 2013;Lee et al, 2013;Zhang et al, 2015a, b;Dickens et al, 2016). Despite the theoretical basis for their effectiveness, additional studies are needed to verify the true risks and benefits of programs based on altered mosquitoes.…”

Section: Control and Prophylaxismentioning

confidence: 99%

A scoping review of Chikungunya virus infection: epidemiology, clinical characteristics, viral co-circulation complications, and control

et al. 2018

View full text Add to dashboard Cite

Chikungunya fever is a mosquito-borne viral illness characterized by a sudden onset of fever associated with joint pains. It was first described in the 1950s during a Chikungunya virus (CHIKV) outbreak in southern Tanzania and has since (re-) emerged and spread to several other geographical areas, reaching large populations and causing massive epidemics. In recent years, CHIKV has gained considerable attention due to its quick spread to the Caribbean and then in the Americas, with many cases reported between 2014 and 2017. CHIKV has further garnered attention due to the clinical diagnostic difficulties when Zika (ZIKV) and dengue (DENV) viruses are simultaneously present. In this review, topical CHIKV-related issues, such as epidemiology and transmission, are examined. The different manifestations of infection (acute, chronic and atypical) are described and a particular focus is placed upon the diagnostic handling in the case of ZIKV and DENV co-circulating. Natural and synthetic compounds under evaluation for treatment of chikungunya disease, including drugs already licensed for other purposes, are also discussed. Finally, previous and current vaccine strategies, as well as the control of the CHIKV transmission through an integrated vector management, are reviewed in some detail.

show abstract

“…In terms of management, genetic techniques to eradicate mosquitos (strains with wingless females, transgenic strains) have recently received much attention (Paupy et al 2009, Sutherland et al 2011, Alphey et al 2013. Some methods developed for mosquitos spread genes through a population despite the genes conferring a reproductive disadvantage, and are meanwhile also explored for alien plants (Hodgins et al 2009).…”

Section: Invasion Stages and Implications For Managementmentioning

confidence: 99%

Alien species and public health impacts in Europe: a literature review

Schindler¹,

Staska²,

Adam³

et al. 2015

View full text Add to dashboard Cite

Impacts of alien species on human health have recently become a major issue in medical research and invasion ecology, but comprehensive assessments of this subject are largely lacking. Here, we provide a literature review of alien species with public health impacts in Europe based on a systematic search in the Thomson Reuters Web of Science. We detected 77 relevant articles, of which 21 were reviews and 56 were original research articles. The taxonomic focus was on vascular plants (n=31 articles) and dipterans (n=25 articles). The original research articles mainly covered the spread of the study species, while early invasion stages (introduction, establishment) as well as impact and management were less investigated. Alien species of health concern in Europe are mostly introduced as contaminants, and mostly originate from climatically similar regions of the Northern Hemisphere. In those cases (36% of all articles) when information on the trend in range and abundance was provided, this trend was mostly increasing. We detected little information on the severity of the impacts (two articles) and the interaction with climate change (three articles). In 15 original articles (28%) specific management measures were suggested, in only one article the socioeconomic costs were assessed. We conclude that European research on human health impacts of alien species is biased towards few species, and that several important aspects such as early invasion stages, severity of impact and its temporal trends, and the scale of the socioeconomic costs caused are poorly understood. Interdisciplinary projects bridging gaps between ecologists medical researchers, socioeconomists and public health authorities are required to link alien species to severity and trends of impacts, which is a crucial requisite for risk assessment and decision making.

show abstract

Genetic control ofAedesmosquitoes

Cited by 134 publications

References 112 publications

Construction of an efficient genomic editing system with CRISPR/Cas9 in the vector mosquito Aedes albopictus

Construction of an efficient genomic editing system with CRISPR/Cas9 in the vector mosquito Aedes albopictus

A scoping review of Chikungunya virus infection: epidemiology, clinical characteristics, viral co-circulation complications, and control

Alien species and public health impacts in Europe: a literature review

Contact Info

Product

Resources

About