Genetic control of <i>Plutella xylostella</i> in omics era

Chen, Wei; Yang, Fei-Ying; Xu, Xuejiao; Kumar, Uttam; He, Weiyi; You, Minsheng

doi:10.1002/arch.21621

Cited by 8 publications

(4 citation statements)

References 38 publications

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“…CRISPR/Cas9 is a genome editing tool that has recently been exploited as an alternative for pest management [61][62][63]. In this study, we hypothesized that OfAbd-A and OfUbx genes modulate the morphological identity and development of thorax and abdomen in O. furnacalis.…”

Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Mutagenesis of Abdominal-A and Ultrabithorax in the Asian Corn Borer, Ostrinia furnacalis

Merchant

et al. 2022

Insects

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(1) Background: Abdominal-A (Abd-A) and Ultrabithorax (Ubx) are homeotic genes that determine the identity and morphology of the thorax and abdomen in insects. The Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Pyralidae), is a devastating maize pest throughout Asia, the Western Pacific, and Australia. Building on previous knowledge, we hypothesized that the knockout of Abd-A and Ubx would disrupt the abdominal body planning in O. furnacalis. (2) Methods: CRISPR/Cas9-targeted mutagenesis was employed to decipher the functions of these homeotic genes. (3) Results: Knockout insects demonstrated classical homeotic transformations. Specifically, the mutagenesis of OfAbd-A resulted in: (1) Fused segments and segmental twist during the larval stage; (2) Embryonic lethality; and (3) The pleiotropic upregulation of other homeotic genes, including Lab, Pd, Dfd, Antp, and Abd-B. The mutagenesis of OfUbx led to: (1) Severe defects in the wing pads, which limited the ability of the adults to fly and mate; (2) Female sterility; and (3) The pleiotropic upregulation of other homeotic genes, including Dfd, Abd-B, and Wnt1. (4) Conclusions: These combined results not only support our hypothesis, but they also strengthen the potential of using homeotic genes as molecular targets for the genetic control of this global insect pest.

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Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Mutagenesis of Abdominal-A and Ultrabithorax in the Asian Corn Borer, Ostrinia furnacalis

Merchant

et al. 2022

Insects

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“…Moreover, CRISPR/Cas9 has been successfully applied to enhance microalgae-based water treatment, improving biomass productivity, tolerance to abiotic stressors, and increasing lipid content for environmental biofuels [100]. Additionally, the self-sustaining method based on the CRISPR/Cas9 system shows potential for pest management, which is crucial for sustainable agricultural practices [101]. Farmers' attitudes toward CRISPR/Cas9, particularly in blast-resistant rice, indicate that new breeding techniques such as CRISPR/Cas9 could represent a valuable solution for achieving sustainable agricultural systems [102].…”

Section: Crispr/cas9 For Conservation and Sustainabilitymentioning

confidence: 99%

Advances in Environmental Biotechnology with CRISPR/Cas9: Bibliometric Review and Cutting-Edge Applications

Verdezoto-Prado,

Chicaiza-Ortiz,

Mejía-Pérez

et al. 2024

Preprint

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CRISPR/Cas9 has emerged as the predominant method for genome editing due to its cost-effectiveness and broad applicability, playing a crucial role in advancing sustainable practices across various sectors. This systematic review leverages the PRISMA methodology to explore CRISPR/Cas9's impact on environmental protection, thereby supporting the Sustainable Development Goals (SDGs). Analyzing data from the Web of Science, the review found significant growth in related publications, with a 30% increase since 2014, predominantly from the US, China, Germany, and the UK. The study categorizes the scientific developments into three trends: advancements in agriculture, gene editing techniques, and biofuel production. Key discussions include the use of CRISPR/Cas9 in developing fourth-generation biofuels and environmental biosensors, as well as its applications in enhancing genetic resilience and controlling invasive species. These innovations highlight CRISPR/Cas9's potential in promoting sustainable resource management and energy generation, marking a pivotal contribution to ecological conservation and sustainability efforts.

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“…In this strategy, transgenic insects can survive solely on a diet supplemented with an antidote. However, without an antidote, they perish due to the presence of the lethal gene when released into the wild. , …”

Section: Application Prospects For P Xylostella By Targeting the Pxgs...mentioning

confidence: 99%

“…However, without an antidote, they perish due to the presence of the lethal gene when released into the wild. 119,120 The application of gene drive strategies also poses challenges, such as unintended ecological consequences. Introducing genetically modified organisms into ecosystems may have unexpected impacts on the environment and nontarget species.…”

Section: The Role Of Pxgsss In Cry1acmentioning

confidence: 99%

New Insights into the Plutella xylostella Detoxifying Enzymes: Sequence Evolution, Structural Similarity, Functional Diversity, and Application Prospects of Glucosinolate Sulfatases

Chen

Amir

Yuan

et al. 2023

J. Agric. Food Chem.

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Brassica plants have glucosinolate (GLs)-myrosinase defense mechanisms to deter herbivores. However, Plutella xylostella specifically feeds on Brassica vegetables. The larvae possess three glucosinolate sulfatases (PxGSS1−3) that compete with plant myrosinase for shared GLs substrates and produce nontoxic desulfo-GLs (deGLs). Although PxGSSs are considered potential targets for pest control, the lack of a comprehensive review has hindered the development of PxGSSs-targeted pest control methods. Recent advances in integrative multi-omics analysis, substrate-enzyme kinetics, and molecular biological techniques have elucidated the evolutionary origin and functional diversity of these three PxGSSs. This review summarizes research progress on PxGSSs over the past 20 years, covering sequence properties, evolution, protein modification, enzyme activity, structural variation, substrate specificity, and interaction scenarios based on functional diversity. Finally, we discussed the potential applications of PxGSSstargeted pest control technologies driven by artificial intelligence, including CRISPR/Cas9-mediated gene drive, transgenic plantmediated RNAi, small-molecule inhibitors, and peptide inhibitors. These technologies have the potential to overcome current management challenges and promote the development and field application of PxGSSs-targeted pest control.

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Genetic control of Plutella xylostella in omics era

Cited by 8 publications

References 38 publications

CRISPR/Cas9-Mediated Mutagenesis of Abdominal-A and Ultrabithorax in the Asian Corn Borer, Ostrinia furnacalis

CRISPR/Cas9-Mediated Mutagenesis of Abdominal-A and Ultrabithorax in the Asian Corn Borer, Ostrinia furnacalis

Advances in Environmental Biotechnology with CRISPR/Cas9: Bibliometric Review and Cutting-Edge Applications

New Insights into the Plutella xylostella Detoxifying Enzymes: Sequence Evolution, Structural Similarity, Functional Diversity, and Application Prospects of Glucosinolate Sulfatases

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