1988
DOI: 10.1002/jobm.3620280502
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Genetic control of Yarrowia lipolytica fatty acid synthetase biosynthesis and function

Abstract: Yarrowia lipolytica, like other lower fungi, has a fatty acid synthetase complex (FAS) with an alpha 6 beta 6 molecular structure. Both subunits are multifunctional proteins each with a molecular weight of more then 200,000 daltons. A collection of FAS-deficient) Y. lipolytica mutants was isolated and characterized by both genetic complementation and enzyme activity measurements. It was found that the three acyl transferases (acetyl-, malonyl- and palmityl-transacylation) together with the enoyl reductase doma… Show more

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Cited by 22 publications
(13 citation statements)
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“…Subsequently, acetyl-Sp and malonyl-Sc thioesters condense to enzyme-bound acetoacetate, thereby initiating the elongation cycle. Isolation and sequencing of the two S. cerevisiae FAS genes, FAS1 and FAS2, subsequently confirmed the chemical structure and location of the two thiol and two hydroxyl acylation sites in yeast FAS (23,67,94,138,141,142). SHp was identified as Cys1305 in the KS domain of FAS2.…”
Section: Fas Catalytic Centersmentioning
confidence: 78%
“…Subsequently, acetyl-Sp and malonyl-Sc thioesters condense to enzyme-bound acetoacetate, thereby initiating the elongation cycle. Isolation and sequencing of the two S. cerevisiae FAS genes, FAS1 and FAS2, subsequently confirmed the chemical structure and location of the two thiol and two hydroxyl acylation sites in yeast FAS (23,67,94,138,141,142). SHp was identified as Cys1305 in the KS domain of FAS2.…”
Section: Fas Catalytic Centersmentioning
confidence: 78%
“…Both the latter have an ~t613, structure (54,64). The catalytic mechanisms appear to be very similar whereby the condensation reaction is carried out in these three organisms (1,9,20,29,33,34,64,66).…”
Section: Discussionmentioning
confidence: 99%
“…Briefly, two partial reactions take place: the acyl component of an activated acyl primer is transferred to a cystcine residue of the I$-ketoacyl synthase and then condensed with an activated malonyl donor with the concomitant release of CO2 and the free enzyme. In S. cerevisiae this catalytic activity is a function of the ~t polypeptide determined by the FAS2 gene which has recently been sequenced (54). When its derived primary structure is compared to that determined by the E. colifabB gene an identical sequence of five amino acids (Ala-Cys-Ala-ThrSer) is found (Fig.…”
Section: Discussionmentioning
confidence: 99%
“…3). As an acyl donor, fatty acylCoA is mostly supplied by fatty acid synthase system (FAS) (Schweizer et al 1988;Wang et al 2011). In addition, acetyl-CoA and malonyl-CoA are essential carbon donor molecules for TAG biosynthesis.…”
Section: Prediction Of Metabolic Engineering Strategies For Improvingmentioning
confidence: 99%