Genetic Control of the Immune Response

Abstract

Eleven strains of mice bearing recombinant H-2 chromosomes derived from known crossover events between known H-2 types were immunized with a series of branched, multichain, synthetic polypeptide antigens [(T,G)-A--L, (H,G)-A--L, and (Phe,G)-A--L]. Results with nine of the eleven H-2 recombinants indicated that the gene(s) controlling immune response to these synthetic polypeptides (Ir-1) is on the centromeric or H-2K part of the recombinant H-2 chromosome. Results with two of the eleven recombinant H-2 chromosomes indicated that Ir-1 was on the telomeric or H-2D part of the recombinant H-2 chromosome. Both of these recombinants were derived from crossovers between the H-2K locus and the Ss-Slp locus near the center of the H-2 region. One of these recombinants, H-2y, was derived from a known single crossover event. These results indicate that Ir-1 lies near the center of the H-2 region between the H-2K locus and the Ss-Slp locus. The results of a four-point linkage test were consistent with these results. In 484 offspring of a cross designed to detect recombinants between H-2 and Ir-1, only two putative recombinants were detected. Both of these recombinants were confirmed by progeny testing. Extensive analysis of one of them has shown that the crossover event occurred within the H-2 region. (Testing of the second recombinant is currently under way.) Thus, in the linkage test, recombinants between H-2 and Ir-1 are in fact intra-H-2 crossovers. These results permit assignment of Ir-1 to a position between the H-2K locus and the Ss-Slp locus.

Genetic control of rheumatoid factor production in the mouse Role of Genes Linked to the Immunoglobulin Heavy Chain Locus and to the Major Histocompatibility Complex

“…129 X B6 (129xB) recombinant inbred (RI) strains developed by Dr. J. L. GuCnet at the Institut Pasteur, Paris, France (7) were rnaintained at our laboratory under clean conventional conditions. The animals used in the present experiments had been inbred for [14][15][16][17][18][19] RF determinations. Anti-IgG2a RF levels were determined either by latex agglutination or by solid phase RIA using polyvinyl wells as described in detail elsewhere (3,9).…”

Serologically Defined and Lymphocyte-Defined Components of the Major Histocompatibility Complex in the Mouse

“…The products of these genes, however, do not seem to be serologically detectable (at least not so far). The loci of the middle segment of the H-2 complex have been shown to be involved in such functions as immune response to synthetic polypeptides (17) and gamma globulin allotypes (25), susceptibility or resistance to tumor viruses (7), production of serum proteins Ss and Sip (26), and as demonstrated here, control of MLC response (13). We have divided the MHC into four regions, It-2K, Ir, Ss, and H-2D, as shown in Fig.…”

GENETIC CONTROL OF THE ANTIBODY RESPONSE TO POLY-L(TYR,GLU)-POLY-D,L-ALA--POLY-L-LYS IN C3H↔CWB TETRAPARENTAL MICE

“…(T,G)-A--L. 5 If an allogeneic effect were the cause of low responder B-cell stimulation some of these mice should have produced high responses. Thus, the response of low responder B cells in tetraparental mice does not appear to be due to an allogeneic effect against these cells.…”