Genetic control of violacein biosynthesis to enable a pigment-based whole-cell lead biosensor

Hui, Chang-ye; Guo, Yan; Liu, Lisa; Zhang, Nai-xing; Gao, Chao-xian; Yang, Xueqin; Yi, Juan

doi:10.1039/d0ra04815a

Cited by 47 publications

(44 citation statements)

References 36 publications

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“…Extensive studies have demonstrated that whole-cell biosensors could detect target molecules in polluted water (Yin et al, 2016 ; Maruthamuthu et al, 2017 ; Cui et al, 2018 ; Hui et al, 2020a ), soil extract (Rasmussen et al, 2000 ; Yoon et al, 2016a , b )(Rasmussen et al, 2000 ; Yoon et al, 2016a , b ), and milk (Kumar et al, 2017 ) by introducing these liquid samples to be tested into the culture system. Biotoxic molecules could be efficiently removed by whole-cell bioadsorbers from polluted water and soils (Wei et al, 2014 ; Maruthamuthu et al, 2017 ; Tang et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Development of Cadmium Multiple-Signal Biosensing and Bioadsorption Systems Based on Artificial Cad Operons

Guo

Hui²,

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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The development of genetic engineering, especially synthetic biology, greatly contributes to the development of novel metal biosensors. The cad operon encoding cadmium resistance was previously characterized from Pseudomonas putida. In this study, single-, dual-, and triple-signal output Cd(II) biosensors were successfully developed using artificial translationally coupled cad operons. Sensitivity, selectivity, and response toward Cd(II) and Hg(II), of three biosensors were all determined. Reporter signals of three biosensors all increased within the range 0.1–3.125 μM Cd(II). Three biosensors responded strongly to Cd(II), and weakly to Hg(II). However, the detection ranges of Cd(II) and Hg(II) do not overlap in all three biosensors. Next, novel Cd(II) biosensing coupled with bioadsorptive artificial cad operons were assembled for the first time. Cd(II)-induced fluorescence emission, enzymatic indication, and Cd(II) binding protein surface display can be achieved simultaneously. This study provides an example of one way to realize multiple signal outputs and bioadsorption based on the redesigned heavy metal resistance operons, which may be a potential strategy for biodetection and removal of toxic metal in the environment, facilitating the study of the mechanism and dynamics of bioremediation.

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Section: Resultsmentioning

confidence: 99%

Development of Cadmium Multiple-Signal Biosensing and Bioadsorption Systems Based on Artificial Cad Operons

Guo

Hui²,

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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“…In addition to the effects of reporters, optimization of detection conditions including the type of culture mediums, induction time and induction duration, was another important factor for enhanced sensitivity [ 13 , 16 , 36 ]. Although whole-cell biosensors responded to low concentration of heavy metal when lag-phase culture of biosensor cells was directly exposed to toxic metal [ 7 , 10 ], exponential phase biosensor cells could produce significantly stronger reporter signals including fluorescent protein, β-galactosidase, and pigment [ 13 , 35 , 37 , 38 ]. Furthermore, logarithmic phase bacterial cultures were usually induced to improve the expression amount of surface-displayed metal binding proteins [ 16 , 18 , 21 , 37 , 39 ].…”

Section: Resultsmentioning

confidence: 99%

Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury

Zhang

Guo

et al. 2021

PLoS ONE

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Mercury exists naturally and mainly as a man-made pollutant in the environment, where it exerts adverse effects on local ecosystems and living organisms. It is important to develop an appropriate synthetic biological device that recognizes, detects and removes the bioavailable fraction of environmental mercury. Both single-signal and double-signal output mercury biosensors were assembled using a natural mer operon as a template. Selectivity and sensitivity of whole-cell biosensors based on artificial mer operons were determined. Three whole-cell biosensors were highly stable at very high concentrations of mercuric chloride, and could detect bioavailable Hg(II) in the concentration range of 6.25–200 μM HgCl2. A novel Hg(II) bioadsorption coupled with biosensing artificial mer operon was assembled. This would allow Hg(II)-induced Hg(II) binding protein cell surface display and green fluorescence emission to be achieved simultaneously while retaining the linear relationship between fluorescent signal and Hg(II) exposure concentration. The present study provides an innovative way to simultaneously detect, quantify, and remove bioavailable heavy metal ions using an artificially reconstructed heavy metal resistance operon.

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“…Thus, a biosensor was constructed that is sensitive to high concentrations of Pb (II) compounds with pigmentation as an output signal. According to the mechanism indicated by the authors, violacein is formed as a result of the conversion of two molecules of endogenous L-tryptophan under the influence of five proteins, which are transcribed and translated as a single polycistronic unit under induction of Pb (II) [9].…”

Section: Introductionmentioning

confidence: 99%

A Blue-Purple Pigment-Producing Bacterium Isolated from the Vezelka River in the City of Belgorod

et al. 2021

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Violacein is a biotechnologically significant secondary metabolite due to its antibacterial, antifungal, and other properties. Isolation, research, and identification of violacein producing strains are of interest for the development of biotechnological processes, in order to enhance the biosynthesis of this compound. The purpose of the present work was to study the properties of a newly isolated bacterium capable of synthesizing blue-purple pigment. An aboriginal bacterium was isolated from the coastal zone of the Vezelka River in the city of Belgorod. Based on chemical and spectrophotometric studies of the crude ethanol extract, the pigment was identified as violacein, and the isolate was assigned to the group of violacein-forming bacteria, which includes bacteria of the genera Chromobacterium, Iodobacter, Janthinobacterium, Duganella, Collimonas, and Massilia. Based on cultural, morphological, tinctorial, physiological, and biochemical properties, as well as analysis of the 16S rRNA gene sequence, the new isolated strain was assigned to the genus Janthinobacterium. The isolated strain is capable of suppressing the growth of a number of fungal and bacterial phytopathogens. For representatives of the genus Janthinobacterium, their inhibitory influence on cyanobacteria was shown for the first time.

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Genetic control of violacein biosynthesis to enable a pigment-based whole-cell lead biosensor

Abstract: Genetically controlled violacein biosynthesis can enable a sensitive, visual, and qualitative biosensor for monitoring the presence of bioavailable lead.

Cited by 47 publications

References 36 publications

Development of Cadmium Multiple-Signal Biosensing and Bioadsorption Systems Based on Artificial Cad Operons

Development of Cadmium Multiple-Signal Biosensing and Bioadsorption Systems Based on Artificial Cad Operons

Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury

A Blue-Purple Pigment-Producing Bacterium Isolated from the Vezelka River in the City of Belgorod

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