Genetic detection and characterization of emerging HoBi-like viruses in archival foetal bovine serum batches

Giammarioli, Monica; Ridpath, Julia F.; Rossi, E.; Bazzucchi, Moira; Casciari, Cristina; Mia, Gian Mario De

doi:10.1016/j.biologicals.2015.05.009

Cited by 42 publications

(33 citation statements)

References 50 publications

(14 reference statements)

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“…The sequences of bovine diarrhea virus were only found on the biosafety cabinet in Room 1, which was used for viral isolation and culture experiments ( Figure 2). The existence of bovine diarrhea viral nucleic acids in fetal bovine serum (FBS) was further confirmed by PCR methods (data not shown) [18], which may represent contamination of bovine serum used for cell culture as reported also in previous studies [18][19][20][21].…”

Section: Detections Of Viral Sequencessupporting

confidence: 69%

Application of next generation sequencing technology on contamination monitoring in microbiology laboratory

Xiao

Zhang

Yang

et al. 2019

Biosafety and Health

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The surveillance and prevention of pathogenic microbiological contamination are the most important tasks of biosafety management in the lab. There is an urgent need to establish an effective and unbiased method to evaluate and monitor such contamination. This study aims to investigate the utility of next generation sequencing (NGS) method to detect possible contamination in the microbiology laboratory. Environmental samples were taken at multiple sites at the lab including the inner site of centrifuge rotor, the bench used for molecular biological tests, the benches of biosafety cabinets used for viral culture, clinical sample pre-treatment and nucleic acids extraction, by scrubbing the sites using sterile flocked swabs. The extracted total nucleic acids were used to construct the libraries for deep sequencing according to the protocol of Ion Torrent platform. At least 1G raw data was obtained for each sample. The reads of viruses and bacteria accounted for 0.01 ± 0.02%, and 77.76 ± 12.53% of total reads respectively. The viral sequences were likely to be derived from gene amplification products, the nucleic acids contaminated in fetal bovine serum. Reads from environmental microorganisms were also identified. Our results suggested that NGS method was capable of monitoring the nucleic acids contaminations from different sources in the lab, demonstrating its promising utility in monitoring and assessing the risk of potential laboratory contamination. The risk of contamination from reagents, remnant DNA and environment should be considered in data analysis and results interpretation.

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Section: Detections Of Viral Sequencessupporting

confidence: 69%

Application of next generation sequencing technology on contamination monitoring in microbiology laboratory

Xiao

Zhang

Yang

et al. 2019

Biosafety and Health

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“…The HoBi‐like sequences analysed in the present study were also classified into genetic groups as defined by Giammarioli, Ridpath et al. (). All of the Brazilian strains were grouped into subtype “a,” and only the strains reported in India were grouped into subtypes c and d (Figure a).…”

Section: Discussionmentioning

confidence: 94%

“…The results presented in this study reinforce the need for vaccines against HoBi‐like viruses, especially in regions where this pestivirus species is prevalent. The possibility of dissemination of HoBi‐like viruses by biological products is a troubling threat, considering that approximately 40% of all HoBi‐like pestiviruses identified in the world are from FBS or contaminated cell cultures (Giammarioli, Ridpath et al., ; Liu, Xia, Baule, & Belák, ; Mao, Li, Zhang, Yang, & Jiang, ; Pelleto et al., ; Schirrmeier, Strebelow, Depner, Hoffmann, & Beer, ; Silveira et al., ; Stalder et al., ; Xia, Vijayaraghavan, Belák, & Liu, ; Xia et al., ). Furthermore, the increasing worldwide identifications of this pestivirus species represent an emerging problem for countries with control and eradication programmes for ruminant pestiviruses.…”

Section: Discussionmentioning

confidence: 99%

HoBi-like is the most prevalent ruminant pestivirus in Northeastern Brazil

Silveira

Baumbach

Weber

et al. 2017

Transbound Emerg Dis

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Summary The ruminant pestiviral species BVDV‐1, BVDV‐2 and BDV, along with the putative species HoBi‐like, may cause substantial economic losses in cattle, sheep and goats. Brazil's large size, variable biomes and wide range of ruminant animal production within different geographic regions suggest that the presence and prevalence of ruminant pestivirus may differ by regions within Brazil. This study investigated the genetic diversity of ruminant pestiviruses and determined the frequency of active infections within two states of the Northeast Region of Brazil, Maranhão and Rio Grande do Norte. Serum samples from 16,621 cattle and 2,672 small ruminants from 569 different herds residing in this region were tested by RT‐PCR followed by DNA sequencing. Seventeen positive cattle were detected (0.1%) from fifteen different herds (2.64%). All isolates were classified as HoBi‐like pestiviruses based on phylogenetic analysis. All small ruminant samples tested negative. The findings presented herein suggest that the Northeast Region of Brazil has a uniquely high prevalence of HoBi‐like viruses. The increasing reports of HoBi‐like viruses detected in cattle in the field suggest that natural infection with these viruses may be more widespread than previously thought. The identification of HoBi‐like viruses as the most prevalent type of ruminant pestivirus circulating in the Northeast Region of Brazil indicates the need for both continued monitoring and determination of the extent of economic losses associated with HoBi‐like virus infections. In addition, it must be taken into account in the choice of diagnostic tests and in vaccine formulations.

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“…The serum is harvested from bovine foetuses taken from pregnant cows during slaughter [47]. Irradiation of FBS is frequently used to eliminate live virus, but no process can be guaranteed as fully effective over time [48]. In a study of 26 commercially available FBS products, all samples contained at least one species of bovine pestivirus by RT-PCR [48].…”

Section: Culture Processmentioning

confidence: 99%

“…Irradiation of FBS is frequently used to eliminate live virus, but no process can be guaranteed as fully effective over time [48]. In a study of 26 commercially available FBS products, all samples contained at least one species of bovine pestivirus by RT-PCR [48]. Fifteen samples tested positive for a putative pestivirus, the “HoBi-like” virus that appears to be related to bovine viral diarrhoea virus (BVDV) [49].…”

Section: Culture Processmentioning

confidence: 99%

Safety of Cultivated Limbal Epithelial Stem Cell Transplantation for Human Corneal Regeneration

Behaegel

Dhubhghaill

Koppen

et al. 2017

Stem Cells International

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Ex vivo cultivated limbal stem cell transplantation is a promising technique for the treatment of limbal stem cell deficiency. While the results of the clinical trials have been extensively reported since the introduction of the technique in 1997, little has been reported regarding the potential health risks associated with production processes and transplantation techniques. Culture procedures require the use of animal and/or human-derived products, which carry the potential of introducing toxic or infectious agents through contamination with known or unknown additives. Protocols vary widely, and the risks depend on the local institutional methods. Good manufacturing practice and xeno-free culture protocols could reduce potential health risks but are not yet a common practice worldwide. In this review, we focus on the safety of both autologous- and allogeneic-cultivated limbal stem cell transplantation, with respect to culture processes, surgical approaches, and postoperative strategies.

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Genetic detection and characterization of emerging HoBi-like viruses in archival foetal bovine serum batches

Cited by 42 publications

References 50 publications

Application of next generation sequencing technology on contamination monitoring in microbiology laboratory

Application of next generation sequencing technology on contamination monitoring in microbiology laboratory

HoBi-like is the most prevalent ruminant pestivirus in Northeastern Brazil

Safety of Cultivated Limbal Epithelial Stem Cell Transplantation for Human Corneal Regeneration

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