Genetic detection of freshwater harmful algal blooms: A review focused on the use of environmental DNA (eDNA) in Microcystis aeruginosa and Prymnesium parvum

Feist, Sheena M.; Lance, Richard F.

doi:10.1016/j.hal.2021.102124

Cited by 23 publications

(10 citation statements)

References 159 publications

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“…From a methodological point of view, this highlights the importance of designing primer sets allowing inclusion of LOOP primers. The detection limits of the developed LAMP techniques are generally between 100–0.1 fg/µl [ 43 , 57 – 59 ]. Our LOD is lower compared to the LOD previously evaluated for the LAMP eDNA detection of the freshwater snail vector of fasciolosis ( Galba truncatula ) [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…DNA amplification can be detected by naked-eye visualization, gel electrophoresis or real-time fluorescence, offering flexibility of use in field settings [ 34 , 36 ]. Recent studies have shown that it is possible to detect eDNA by LAMP [ 40 – 43 ]. An environmental LAMP (eLAMP) was developed to detect the presence of faecal indicator bacteria in water in 1 h, without sophisticated laboratory equipment or highly trained personnel [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

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Development of environmental loop-mediated isothermal amplification (eLAMP) diagnostic tool for Bulinus truncatus field detection

et al. 2023

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Background Global changes are reshaping the distribution of vector-borne diseases by spreading vectors to previously non-endemic areas. Since 2013, urogenital schistosomiasis has emerged in Corsica and threatens European countries. Gastropod vectors release schistosome larvae that can infect humans who come into contact with freshwater bodies. Monitoring schistosomiasis host vectors is a prerequisite to understand and subsequently to control this pathogen transmission. Because malacological surveys are time consuming and require special expertise, the use of a simple molecular method is desirable. Methods The aim of this study is to develop a ready-to-use protocol using the LAMP (loop-mediated isothermal amplification) method to detect environmental DNA of Bulinus truncatus, vector of Schistosoma haematobium. Interestingly, LAMP method possesses all the characteristics required for adaptability to field conditions particularly in low-income countries: speed, simplicity, lyophilized reagents, low cost and robustness against DNA amplification inhibitors. We have tested this new method on Corsican water samples previously analysed by qPCR and ddPCR. Results We demonstrate that our diagnostic tool B. truncatus eLAMP (Bt-eLAMP) can detect the eDNA of Bulinus truncatus as effectively as the two other methods. Bt-eLAMP can even detect 1/4 of positive samples not detectable by qPCR. Moreover, the complete Bt-eLAMP protocol (sampling, sample pre-process, amplification and revelation) does not require sophisticated equipment and can be done in 1 ½ h. Conclusions LAMP detection of environmental DNA provides large-scale sensitive surveillance of urogenital schistosomiasis possible by identifying potentially threatened areas. More generally, eLAMP method has great potential in vector-borne diseases and ecology. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of environmental loop-mediated isothermal amplification (eLAMP) diagnostic tool for Bulinus truncatus field detection

et al. 2023

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“…These efforts may reveal specific strains associated with bloom hotspots or uncover population structure that points to environmental gradients that drive adaptation to the unique environment of Lake Superior. Mapping the genetic landscape of D. lemmermannii can illuminate potential propagule sources (e.g., distinct strains clustered at river mouths) and contribute to effective monitoring efforts by identifying strains whose presence is correlated with bloom frequency (Feist and Lance, 2021). Such a map would also provide a framework for future comparative genomic studies to identify the genetic basis of ecological adaptations (e.g., cold-tolerance) that allow previously transient microbes to thrive in Lake Superior, and be useful for projecting future trends as climate change and other anthropogenic impacts intensify.…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal dynamics of cyanobacteriumDolichospermum lemmermanniipopulations in a bloom-prone region of Lake Superior

Wood,

Callaghan,

Loiselle

et al. 2024

Preprint

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Cyanobacterial Harmful Algal Blooms (cHABs) are increasingly common in marine and freshwater environments, including the Laurentian Great Lakes (LGL). Lake Superior has seen two large-scale cyanobacterial blooms (2012 and 2018) along the Wisconsin shoreline west of the Apostle Islands, caused by the cyanobacterium Dolichospermum lemmermannii. The drivers of bloom formation in Lake Superior are not yet certain, with many factors known to trigger blooms elsewhere in the LGL being absent in Lake Superior. Furthermore, little is known about D. lemmermannii spatial structure or phenology. Thus, we sought to track the seasonal population dynamics of D. lemmermannii to shed light on its growth, physiology, and abundance. In 2021, we used 16S rRNA amplicon and shotgun metagenomic sequencing to characterize spatio-temporal patterns of D. lemmermannii abundance and diversity along the bloom-prone Wisconsin shore of Lake Superior. In 2022, we performed net tows and direct colony counts in another localized area. No large-scale bloom event was observed during either year, though several smaller blooms were observed. D. lemmermannii abundances were low at nearly all sites and sampling times. Spikes in abundance occurred in July and September, particularly near Siskiwit Bay, a hotspot of bloom formation. We also observed a seasonal shift in heterocyte and akinete abundance indicative of late-season nutrient limitation. Most striking was the seasonal turnover of D. lemmermannii strains, suggesting strain adaptation to specific environmental conditions. These results offer valuable and actionable insights for managers and provide a foundation for additional work to clarify drivers of bloom formation in Lake Superior.

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“…Researchers gain insights into the overall state of aquatic environments by understanding how environmental toxins affect the development, reproduction, growth, and life cycles of Daphnia (Lurling 2003 ; Herrera et al 2015 ; Schwarzenberger and Martin-Creuzburg 2021 ). Excessive nutrient input to freshwater has increased the frequency of cyanobacterial blooms, which produce biological toxins referred to cyanotoxins that affect the feeding, growth, and life history parameters of the grazer Daphnia (Ma et al 2014 ; Huisman et al 2018 ; Feist and Lance 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Dietary exposure of the water flea Daphnia galeata to microcystin-LR

Kim,

Jeon,

Park

et al. 2024

Animal Cells and Systems

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Harmful substances like the cyanotoxin microcystin-leucine-arginine (MC-LR) are commonly found in eutrophic freshwater environments, posing risks to aquatic organisms. The water flea, Daphnia , is a well-established model organism for environmental toxicology research. Nevertheless, there is currently insufficient research on the genes that respond to MC-LR in Daphnia galeata . This study aimed to gain insights into the notable genes that react significantly to MC-LR. In this study, we generated an extensive RNA-Seq sequences isolated from the D . galeata HK strain, Han River in Korea. This strain was nourished with a diet of the green microalga Chlorella vulgaris and treated with pure MC-LR at a concentration of 36 ug/L. The transcriptome profile in response to the MC-LR treatment was obtained and 336 differentially expressed genes were subjected to Gene Ontology (GO) and euKaryotic Orthologous Groups of proteins analyses. GO enrichment analysis showed that chemical stimulus, amino sugar metabolic and catabolic process, oxidative stress, and detoxification were highly enriched, in reverse, proteolysis and fucosylation were underpresented. Detoxification process related genes such as peroxidase-like, chorion, and thyroid peroxidase-like were enriched for eliminating or neutralizing MC_LR from an organism's body. Furthermore, functional protein classification revealed an upregulation of lipid and inorganic ion transport processes, while amino acid and carbohydrate transport processes were found to be downregulated. These findings offer insights into how organisms respond to ecotoxic stimuli, providing valuable information for understanding adaptation or defense pathways.

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Genetic detection of freshwater harmful algal blooms: A review focused on the use of environmental DNA (eDNA) in Microcystis aeruginosa and Prymnesium parvum

Cited by 23 publications

References 159 publications

Development of environmental loop-mediated isothermal amplification (eLAMP) diagnostic tool for Bulinus truncatus field detection

Development of environmental loop-mediated isothermal amplification (eLAMP) diagnostic tool for Bulinus truncatus field detection

Spatiotemporal dynamics of cyanobacteriumDolichospermum lemmermanniipopulations in a bloom-prone region of Lake Superior

Dietary exposure of the water flea Daphnia galeata to microcystin-LR

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