Genetic determinants of multi-drug resistance in<i>Acinetobacter baumannii</i>at an academic hospital in Pretoria, South Africa

Nogbou, Noel-David; Phofa, D.T.; Nchabeleng, Maphoshane; Musyoki, Andrew Munyalo

doi:10.1101/809129

Cited by 4 publications

(3 citation statements)

References 42 publications

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“…Piperacillin-tazobactam is known to be a key antibiotic against A. baumannii [51]. Contrary to our results (resistance rate of 31.3%), several studies have reported a high prevalence of resistance to piperacillin-tazobactam, ranging from 62 to 100% in Benin, Tanzania, Ghana, Morocco, and South Africa [52,53].…”

Section: Discussioncontrasting

confidence: 99%

Antibiotic Resistance Profiles of Bacteria Isolated from Patients Hospitalized at the Sylvanus Olympio University Teaching Hospital in Lomé, Togo

Dossim,

Bawe,

Dossouvi

et al. 2024

MRJI

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Aims: Bacteria isolated from hospitalized patients are often responsible for healthcare-associated infections (HAI), particularly in developing countries. Thus, this study aimed to determine the antibiotic resistance profiles of bacteria isolated from patients hospitalized at the Sylvanus Olympio University Teaching Hospital (CHUSO) in Lomé, Togo. Study Design: This was a descriptive cross-sectional study carried out on laboratory data collected from January 1, 2018, to December 31, 2019. Methodology: The Kirby-Bauer disc diffusion method was used for antibiotic susceptibility testing and the results were interpreted according to the guidelines of the Antibiogram Committee of the French Society of Microbiology (CA-SFM, 2018). Results: A total of 639 samples were collected, including mainly pus (n = 339; 53.1%) and urine (n = 260; 40.7%). The samples were mainly from pediatrics (n=107; 16.7%), intensive care units (n=73; 11.4%) and surgical emergencies (n=72; 11.3%). A total of 698 bacteria were isolated, including mainly Escherichia coli (n=247, 35.4%), Staphylococcus aureus (n=123, 17.6%), and Klebsiella pneumoniae (n=114, 16.3%). Enterobacteriaceae strains were resistant to almost all antibiotics tested, except amikacin and ertapenem, which had respective resistance rates of 2.8% and 8.4%. None of the P. aeruginosa strains were resistant to piperacillin-tazobactam. whereas 37.9% were resistant to imipenem. Among A. baumannii strains, 4.2% and 22.9% were respectively resistant to amikacin and imipenem, whereas 56.3% were resistant to levofloxacin. Almost all S. aureus strains (99.2%) were resistant to penicillin, whereas only 2.4% were resistant to rifampicin. Of the 698 bacteria isolated, the prevalence of multidrug-resistant bacteria (MDR) was 41.3% (n = 288), whereas the prevalence of ESBL-producing Enterobacteriaceae was 51.3% (201/392). Conclusion: We reported various resistance profiles of bacterial species isolated from hospitalized patients at the CHU Sylvanus Olympio, Lomé, Togo. The study of bacterial ecology and resistance in every prefectural, regional, and university teaching hospital would be of great importance to reduce mortality associated with hospital-acquired infections throughout the Togolese territory.

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Section: Discussioncontrasting

confidence: 99%

Antibiotic Resistance Profiles of Bacteria Isolated from Patients Hospitalized at the Sylvanus Olympio University Teaching Hospital in Lomé, Togo

Dossim,

Bawe,

Dossouvi

et al. 2024

MRJI

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“…Kim et al [47] stated the high A. baumannii prevalence (27.80%) amongst the raw milk samples which is supported by the high antibiotic resistance of isolates against ceftriaxone (4.4%), tetracycline (30.8%), gentamicin (2.9%), and cefotaxime (12.5%). Similarly, in surveys conducted on Iran [48], Brazil [49], Jordan [50], United States [51], Korea [52], and Africa [53], high A. baumannii resistance rates against tetracycline, ampicillin, gentamicin, trimethoprim, ciprofloxacin, and erythromycin were reported. Askari et al [21] stated that the A. baumannii strains isolated from raw meat of animal species harbored the highest resistance rate against tetracycline (82.35%), gentamycin (74.50%), streptomycin (54.90%), cotrimoxazole (70.58%), and trimethoprim (62.74%).…”

Section: Discussionmentioning

confidence: 88%

Prevalence and Phenotypic and Genotypic Patterns of Antibiotic Resistance of Acinetobacter baumannii Strains Isolated from Fish, Shrimp, and Lobster Samples

Hasiri

Rahimi

Momtaz

et al. 2023

Journal of Food Processing and Preservation

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Acinetobacter baumannii has emerged as a significant hospital pathogen, quickly becoming resistant to commonly prescribed antimicrobials. The present survey was done to evaluate the prevalence, antibiotic resistance pattern, and distribution of antibiotic resistance genes amongst the A. baumannii strains isolated from fish, shrimp, and lobster samples. Four-hundred and fifty seafood samples (100 g each) were collected from Shiraz, Iran. Acinetobacter baumannii was determined using culture and biochemical tests. Pattern of antibiotic resistance and distribution of antibiotic resistance genes were determined using the disk diffusion and polymerase chain reaction, respectively. A. baumannii contamination rate amongst the examined seafood samples was 4.44%, with the higher contamination rate of fish samples (7.85%). A. baumannii isolates harbored the maximum resistance rate against tetracycline (85%), ampicillin (85%), gentamicin (70%), and erythromycin (60%). Resistance rates toward trimethoprim-sulfamethoxazole, ciprofloxacin, ceftazidime, and azithromycin were 55%, 45%, 35%, and 30%, respectively. The minimum rates of resistance were obtained against imipenem (10%) and chloramphenicol (15%). The most commonly detected antibiotic resistance genes were blaCITM (75%), blaSHV (70%), tetA (70%), qnrA (55%), blaVIM (50%), and aac(3)-IV (50%). aadA1, sul1, dfrA1, qnr, blaVIM, blaSIM, blaOXA-51, blaOXA-23, and blaOXA-58 genes were detected in 40%, 30%, 45%, 50%, 35%, 25%, 30%, and 20% of isolates, respectively. The role of seafood samples as a potential reservoirs of antibiotic-resistant A. baumannii strains was determined. However, further investigations are required to identify additional epidemiological features of A. baumannii in seafood samples.

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“…In keeping with this, this pathogen's role in seafood samples is potentially unknown, and further research is required to understand this fact. High prevalence of resistance of A. baumannii strains toward commonly-used antimicrobials, particularly tetracycline, gentamicin, ciprofloxacin, trimethoprim, streptomycin, and erythromycin was reported from Brazil [41], Iran [42], United States [43], Jordan [44], Africa [45], and Korea [46].As antibiotic-resistant A. baumannii was detected in large number of seafood samples. Proper monitoring of the seafood microbial quality should perform at the site of fishing and also after their transport.…”

Section: Discussionmentioning

confidence: 99%

The First Report of the Antimicrobial Resistance of Acinetobacter baumannii Isolates from Seafood Samples

et al. 2021

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A cinetobacterbaumannii is a newly-launched bacterium responsible for rare cases of diarrhea and foodborne diseases. This survey was performed to study the prevalence and antimicrobial resistance of A. baumannii isolates recovered from fish, shrimp, and lobster. Five-hundred seafood samples were collected and cultured for isolation of A. baumannii. A. baumannii isolates were identified by biochemical tests and further confirmed by the PCR. The antimicrobial resistance of isolates was studied using disk diffusion test. Twenty-eight out of 500 (5.6%) seafood samples were contaminated with A. baumannii. Fish had the highest prevalence of A. baumannii (10%), while lobster had the lowest (2.5%). A. baumannii isolates harbored the highest incidence of resistance toward tetracycline (75%), gentamicin (71.4%), ciprofloxacin (64.2%), trimethoprim (57.1%), streptomycin (53.5%), and erythromycin (50%) antimicrobial agents. The lowest incidence of resistance was obtained against chloramphenicol (3.5%) and imipenem (7.1%) antimicrobial agents. This is the first report of isolation and identification and antimicrobial resistance pattern of A. baumannii in seafood samples globally. The role of seafood samples as reservoirs of antimicrobial-resistant A. baumannii was determined. Complete cooking of seafood and prevention from their consumption in raw form can diminish the risk of A. baumannii foodborne diseases.

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Genetic determinants of multi-drug resistance inAcinetobacter baumanniiat an academic hospital in Pretoria, South Africa

Cited by 4 publications

References 42 publications

Antibiotic Resistance Profiles of Bacteria Isolated from Patients Hospitalized at the Sylvanus Olympio University Teaching Hospital in Lomé, Togo

Antibiotic Resistance Profiles of Bacteria Isolated from Patients Hospitalized at the Sylvanus Olympio University Teaching Hospital in Lomé, Togo

Prevalence and Phenotypic and Genotypic Patterns of Antibiotic Resistance of Acinetobacter baumannii Strains Isolated from Fish, Shrimp, and Lobster Samples

The First Report of the Antimicrobial Resistance of Acinetobacter baumannii Isolates from Seafood Samples

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