Genetic diagnosis of a rare myrmecochorous species, Plagiorhegma dubium (Berberidaceae): Historical genetic bottlenecks and strong spatial structures among populations

Abstract

Distribution of genetic variation over time and space is relevant to demographic histories and tightly linked to ecological disturbances as well as evolutionary potential of an organism. Therefore, understanding the pattern of genetic diversity is a primary step in conservation and management projects for rare and threatened plant species. We used eight microsatellite markers to examine the level of genetic diversity, spatial structure, and demographic history of Plagiorhegma dubium, a rare myrmecochorous herb, populations sampled across northeast Asia and Siberia. We found low within‐population genetic variation associated with historical bottlenecks. Although pairwise F ST values were not much higher than the ones found in similar life form species, STRUCTURE and PCoA revealed a clear broadscale spatial pattern of genetic structure. Bayesian clustering (best K = 6) and PCoA identified three populations that are distinctive from neighboring populations in the Korean peninsula, which suggests potential units for conservation and management plans in Korea. MIGRATE‐N and BAYESASS showed that both contemporary (0.003–0.045) and historical migration rates (2 × e−5−4.6 × e−4) were low. Our findings provide a good example, where genetic considerations should be integrated for conservation and management plans of rare and threatened species.

Mahonia vs. Berberis Unloaded: Generic Delimitation and Infrafamilial Classification of Berberidaceae Based on Plastid Phylogenomics

“…would likely further exacerbate their critical conservation status given the stereotypical impression of Berberis as agricultural weeds. Additionally, because both P. dubium ( Lee et al, 2018 ) and S. hexandrum ( Liu W. et al, 2016 ) are also rare and exploited for traditional medicines, recognizing and elevating these two distinct species to the generic rank also confers an effective conservation strategy.…”

“…Additionally, because both P. dubium (Lee et al, 2018) and S. hexandrum (Liu W. et al, 2016) are also rare and exploited for traditional medicines, recognizing and elevating these two distinct species to the generic rank also confers an effective conservation strategy.…”

Genetic Diversity on a Rare Terrestrial Orchid, Habenaria linearifolia in South Korea: Implications for Conservation Offered by Genome-Wide Single Nucleotide Polymorphisms

“…Accordingly, the role of population genetic diversity has become increasingly significant in terms of responding to biodiversity loss and subsequent conservation strategies ( des Roches et al, 2021 ; Hohenlohe et al, 2021 ; Stange et al, 2021 ). For example, Lee et al (2018) suggested risk in deleting a rare myrmecochorous herb, Plagiorhegma dubium from the Red List of South Korea based on the pattern of population genetic diversity of simple sequence repeat data despite the recent growth of census population sizes.…”