Genetic difference between Ezo-awabi Haliotis discus hannai and Kuro-awabi H. discus discus populations: Microsatellite-based population analysis in Japanese abalone

Hara, Motoaki; Sekino, Masashi

doi:10.1111/j.1444-2906.2005.01025.x

Cited by 35 publications

(34 citation statements)

References 18 publications

(17 reference statements)

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“…Microsatellite analysis: The source of microsatellite markers used in this study was as follows: two markers suffixed with Hdd in Sekino and Hara (2001), six markers with Hd in Hara and Sekino (2005), 111 markers with Afa and Awb in Sekino et al (2005Sekino et al ( , 2006, and 15 markers with Ahdh in Sekino and Hara (2007). These markers turned out to be informative in at least one of the three mapping families on the basis of our preliminary marker screening.…”

Section: Methodsmentioning

confidence: 99%

Linkage Maps for the Pacific Abalone (Genus Haliotis) Based on Microsatellite DNA Markers

Sekino

Hara

2007

Genetics

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This study presents linkage maps for the Pacific abalone (Haliotis discus hannai) based on 180 microsatellite DNA markers. Linkage mapping was performed using three F 1 outbred families, and a composite linkage map for each sex was generated by incorporating map information from the multiple families. A total of 160 markers are placed on the consolidated female map and 167 markers on the male map. The numbers of linkage groups in the composite female and male maps are 19 and 18, respectively; however, by aligning the two maps, 18 linkage groups are formed, which are consistent with the haploid chromosome number of H. discus hannai. The female map spans 888.1 cM (Kosambi) with an average spacing of 6.3 cM; the male map spans 702.4 cM with an average spacing of 4.7 cM. However, we encountered several linkage groups that show a high level of heterogeneity in recombination rate between families even within the same sex, which reduces the precision of the consolidated maps. Nevertheless, we suggest that the composite maps are of significant potential use as a scaffold to further extend the coverage of the H. discus hannai genome with additional markers.

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Section: Methodsmentioning

confidence: 99%

Linkage Maps for the Pacific Abalone (Genus Haliotis) Based on Microsatellite DNA Markers

Sekino

Hara

2007

Genetics

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“…This finding is in agreement with the fact that the hatchery stocks are characterized by having reduced genetic variation due to the random genetic drift that exists in the aquaculture environment. Alternatively, the possibility that H. d. discus (Camacjeonbok), which is a sister subspecies of H. d. hannai (Cham-jeonbok), constitutes this population cannot be excluded because H. d. discus is a highly valuable fishery resource in Jeju, Korea, and clear discrimination based on the morphological characteristics between the two species is difficult Hara and Sekino, 2005). Previously, it was reported that microsatellite markers developed from H. d. hannai provided high resolution for the analysis of the genetic differences between H. d. hannai and H. d. discus populations (Hara and Sekino, 2005).…”

Section: Discussionmentioning

confidence: 99%

Population genetics of the Pacific abalone (Haliotis discus hannai) in Korea inferred from microsatellite marker analysis

Lee²,

Park³

2012

Genet. Mol. Res.

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ABSTRACT. Populations of the Pacific abalone, Haliotis discus hannai, have been severely overexploited over the past few decades in Korea. Information regarding the levels of genetic variability and structure within populations is insufficient for the development of effective strategies for conservation of genetic diversity of this species. To assess the genetic status of this species, we examined variation at six microsatellite loci in 842 individuals of Pacific abalone collected from three hatchery stocks of the main aquaculture areas and eight wild populations, which were two from the East Sea, two from the West Sea and three from the South Sea. High levels of polymorphism at these microsatellite loci were found in both the wild and hatchery populations. The genetic variation in the hatchery stocks [overall number of alleles (N A ) = 24.00; allelic richness (A R ) = 19.71; observed heterozygosity (H O ) = 0.733] was similar to that of the wild (overall N A = 28.13; A R = 22.62; H O = 0.775) populations. Low levels of inbreeding and significant Hardy-Weinberg equilibrium deviations were detected in both the wild and hatchery populations. Significant F ST values were observed for the hatchery stocks and in most cases between the wild and hatchery populations (overall F ST = 0.017, P < 0.01); however, only a Population genetics of Pacific abalone in Korea minor portion of the genetic diversity was distributed between the wild and hatchery populations. These results reflect intensive seedling and stocking practices. This preliminary study showed genetic separation between the eastern and pooled western and southern wild populations in Korea, which was based on the F ST value, phylogenetic tree clustering, PCA and MDS analyses, structure analysis, and AMOVA. This strong biogeographic structure of H. d. hannai in Korea may be considered to be independent management units. This study demonstrates the feasibility of microsatellite analyses for the monitoring of genetic diversity and for revealing the population structure of the wild Pacific abalone. This information will be useful for the proper management and conservation of H. d. hannai in Korea.

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“…Later attempts of nucleotide sequencing analyses (sperm lysin cDNA sequences: Lee and Vacquier 1995;mitochondrial genes: An et al 2005) only resulted in a support of the limited genetic divergence found in the allozyme study. A recent population analysis based on selectively neutral microsatellites (but see Zhang and Hewitt 2003), however, unveiled a high allopatric divergence between H. discus hannai and H. discus discus (Hara and Sekino 2005), which had been shadowed in the previous studies, leaving a possibility that microsatellites may elicit another genetic aspect of the Pacific abalone species complex.…”

Section: Introductionmentioning

confidence: 94%

“…We applied the H. discus hannai microsatellites previously reported (Hara and Sekino 2005) together with those developed in this study to the individual assignment tests. In such a way we found several lines of evidence to delineate the Pacific abalone species complex at the individual level.…”

Section: Introductionmentioning

confidence: 99%

Individual assignment tests proved genetic boundaries in a species complex of Pacific abalone (genus Haliotis)

Sekino

Hara

2006

Conserv Genet

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Abstracts We conducted this study to find genetic evidence to distinguish the members of Pacific abalone species complex (Haliotis discus hannai, H. discus discus, H. madaka, and H. gigantea) based on microsatellite DNA markers, illustrating the potential of microsatellites for species-assignment. First, we addressed the transferability of H. discus hannai microsatellites to the three other members of Pacific abalone and five additional species (H. diversicolor aquatilis, H. midae, H. corrugata, H. fulgens, and H. rubra). Second, using the microsatellites we applied two types of individual assignment testing (the distance-based assignment and Bayesian modelbased clustering) to individuals from the Pacific abalone species. A total of 24 microsatellites were subjected to PCR trials for nine Haliotis species, and the cross-species amplification performance of these markers turned out to drop precipitously even for less divergent congeners. Within the Pacific abalone species complex, four of the 24 markers were not transferable to H. gigantea, suggesting a solid genetic boundary between H. gigantea and H. discus hannai, H. discus discus, and H. madaka. Among the three latter abalones, both assignment tests achieved approximately 90% or more success rate of assignment. The feasibility of the microsatellite markers to classify species sheds light on the genetic management of the Pacific abalone species complex.

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Genetic difference between Ezo-awabi Haliotis discus hannai and Kuro-awabi H. discus discus populations: Microsatellite-based population analysis in Japanese abalone

Cited by 35 publications

References 18 publications

Linkage Maps for the Pacific Abalone (Genus Haliotis) Based on Microsatellite DNA Markers

Linkage Maps for the Pacific Abalone (Genus Haliotis) Based on Microsatellite DNA Markers

Population genetics of the Pacific abalone (Haliotis discus hannai) in Korea inferred from microsatellite marker analysis

Individual assignment tests proved genetic boundaries in a species complex of Pacific abalone (genus Haliotis)

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