Genetic differences between eastern and western populations of the alfalfa weevil

Sell, D. K.; Armbrust, Edward J.; Whitt, Gregory S.

doi:10.1093/oxfordjournals.jhered.a108880

Cited by 3 publications

(6 citation statements)

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“…Our study with 22 gene loci of six geographical populations of the alfalfa weevil shows that the genetic distance between the eastern, western, and Egyptian weevils are relatively small and are within the expected range of population variations. This conclusion is at variance with the previous finding of Sell et al (1978) who described certain genetic variations between eastern and western weevil strains based on five gene loci surveyed. Considering the small sample sizes and the few gene loci they measured, the differences they observed were not unexpected.…”

Section: Discussioncontrasting

confidence: 99%

“…Our 0.78 allele (equivalent to their EST-a) was not observed in the western and Egyptian weevils, but was found at a low frequency in the eastern weevils. This result is in agreement with the finding of Sell et al (1978). However, we were able to detect the 0.90 allele (equivalent to their EST-b) in all weevil populations.…”

Section: Resultssupporting

confidence: 93%

“…EST-I consisted of four alleles, while EST-2 and EST-3 had two alleles each. EST-1 appeared to be identical to the sole EST locus described by Sell et al (1978). Their most common allele, EST-c, was the same as the 1.0 allele of our EST-1 (frequency 0.795-0.877).…”

Section: Resultsmentioning

confidence: 48%

“…Frozen samples had much less defined staining, but were scorable. The two M DH loci described by Sell et al (1978) migrate cathodally and were not included in our study.…”

Section: Resultsmentioning

confidence: 99%

“…Recent studies on isozymes of Coleoptera have been reviewed by Jacobson & Hsiao (1983). Previously, Sell et aL (1978) had examined five gene loci from four enzymes by starch gel electrophoresis to assess genetic variations between eastern and western weevil populations. Our study includes 22 gene loci from 12 enzymes of the eastern, western and Egyptian weevil populations and seeks to define the genetic differences between weevil strains by direct measurement of genetic distances.…”

Section: Introductionmentioning

confidence: 99%

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Discrimination of alfalfa weevil strains by allozyme analysis

Hsiao

Stutz

1985

Entomologia Exp Applicata

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Allozyme profiles of eastern weevils (Beltsville, Maryland; Washington Co., Illinois), western weevils (Logan and St. George, Utah), and Egyptian weevils (Yuma, Arizona; Westmorland, California) were compiled by acrylamide gel electrophoresis. Twenty‐two gene loci from 12 enzymes (ACPH, ADH, AMY, AO, EST, GOT, G‐6PDH, MDH, ME, SOD, TYR, XDH) were analyzed. Mean heterozygosity of these populations was 0.231, with an average proportion of polymorphic loci of 0.536. The mean genetic distance of all weevil populations was 0.033 and the fixation index was 0.024. Diagnostic loci were found which could distinguish western weevils from eastern and Egyptian weevils. The small genetic distance between the eastern and Egyptian weevils suggests that they may be the same strain and are certainly different from the western weevil strain. Based on this and other evidence, we conclude that all weevil strains in the United States are Hypera postica (Gyllenhal), and that the use of H. brunneipennis (Boheman) for the Egyptian alfalfa weevil of North America should be discontinued. RÉSUMÉ Séparation par analyse des allozymes de souches d'Hypera de la luzerne Les allozymes d'Hypera de l'est des USA (Beltsville, Maryland; Washington Co., Illinois), de l'ouest des USA (Logan et St Georges, Utah) et 'égyptiens' (Yuma, Arizona; Westmorland, Californie) ont été analysés par électrophorèse sur gel d'acrylamide. L'étude a porté sur 22 loci de 12 enzymes (ACPH, ADH, AMY, AO, EST, GOT, G‐6PDH, MDH, ME, SOD, TYR, XDH). L'hétérozygotie moyenne de la population était 0.231, avec une moyenne de loci polymorphes de 0.536. La distance génétique moyenne de l'ensemble des populations était de 0.033 et l'indice de fixation de 0.024. Des loci caractéristiques ont été trouvés qui pourraient permettre de distinguer les Hypera occidentaux des orientaux et des 'égyptiens'. La faible distance génétique entre les Hypera orientaux et 'égyptiens' suggère qu'ils appartiennent à la měme souche et sont certainement différents des occidentaux. A partir de cela et d'autres éléments, nous concluons que tous les Hypera des USA sont H. postica Gyllenhal et que l'utilisation d'H. brunneipennis Boheman pour désigner les Hypera 'égyptiens' d'Amérique du Nord doit ětre abandonnée.

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Section: Discussioncontrasting

confidence: 99%

Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 48%

“…Frozen samples had much less defined staining, but were scorable. The two M DH loci described by Sell et al (1978) migrate cathodally and were not included in our study.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Discrimination of alfalfa weevil strains by allozyme analysis

Hsiao

Stutz

1985

Entomologia Exp Applicata

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Life-History Strategies and the Genetic Structure of Phytophagous Insect Populations

Peterson

Denno

1998

Genetic Structure and Local Adaptation in Natural Insect Populations

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Molecular Differentiation of Alfalfa Weevil Strains (Coleoptera: Curculionidae)

Erney¹,

Pruess²,

Danielson³

et al. 1996

Annals of the Entomological Society of America

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Mitochondrial DNA was amplified and sequenced from eastern, western, and Egyptian strains of alfalfa weevil, Hypera postica (Gyllenhal). Eastern and Egyptian weevils differed at only 2 nucleotide sites in 1,031 base pairs sequenced; western weevils differed by 5% sequence divergence. Three restriction sites were identified which separated eastern and western haplotypes. No intrastrain polymorphism was detected in 150 weevils from Nebraska. Collections from Lincoln in eastern Nebraska and Scottsbluff in western Nebraska were fixed for the eastern and western haplotypes, respectively. Eastern and western haplotypes were found together in the same fields in a broad overlap region in central Nebraska.

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Genetic differences between eastern and western populations of the alfalfa weevil

Cited by 3 publications

References 0 publications

Discrimination of alfalfa weevil strains by allozyme analysis

Discrimination of alfalfa weevil strains by allozyme analysis

Life-History Strategies and the Genetic Structure of Phytophagous Insect Populations

Molecular Differentiation of Alfalfa Weevil Strains (Coleoptera: Curculionidae)

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