Genetic differences between Tunisian camel and sheep strains of the cestode<i>Echinococcus granulosus</i>revealed by SSCP

Oudni-M’rad, Myriam; Cabaret, Jacques; M’rad, Selim; Bouzid, W.; Mekki, Mongi; Belguith, M.; Sayadi, T.; Nouri, Abdellatif; Lahmar, Samia; Azaiez, R.; Mezhoud, Habib; Babba, Hamouda

doi:10.1051/parasite/2006132131

Cited by 10 publications

(5 citation statements)

References 28 publications

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“…The genetic distances were not related to geographic distances, which is indicative of local divergent evolutions rather than isolation by distance. This is in agreement with those reported by Oudni-M’rad et al [ 52 , 53 ] who described significant genetic variation within the G1 genotype isolates of Sousse and Gafsa in Tunisia based on different genetic markers. No genetic structuration (genetic differences between regions) due to geographic distance could be found by the use of the Mantel test nor the TEX trees in NJ or the ADD tree applications.…”

Section: Discussionsupporting

confidence: 93%

Infection of dogs with Echinococcus granulosus: causes and consequences in an hyperendemic area

et al. 2015

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BackgroundTunisia is a hyper endemic country for human echinococcosis. The infection is transmitted via the eggs of Echinococcus granulosus which are passed in the faeces of the definitive canid host.MethodsThis study evaluated the contamination rate of the dog faeces in different climatic conditions at eight different geographic regions throughout Tunisia. Dog faecal samples were collected from the soil and the Echinococcus eggs were identified using microscopic and molecular (Eg1121/1122 PCR, Egss1 PCR and Nad1 PCR-RFLP) tools.ResultsThe contamination index of dog faeces by E. granulosus eggs ranged from 8.3% to 41.3% depending on the region. Comparisons of the dog faecal contamination rate against human incidence found them to be independent. Neither human prevalence nor dog contamination index appeared to be related to climatic conditions or geographic characteristics. The genetic variability of E. granulosus samples was different within each region but was not related to geographic distance which is indicative of local divergent evolutions rather than isolation by distance.ConclusionsA high environmental dog contamination index does not necessarily correspond to high prevalence in humans as transmission is strongly linked to human behavior and hygiene.

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Section: Discussionsupporting

confidence: 93%

Infection of dogs with Echinococcus granulosus: causes and consequences in an hyperendemic area

et al. 2015

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“…(1990) and Oudni-M'rad et al . (2006). Moreover, in our findings, the 12S rRNA gene seems to show various PCR-SSCP patterns (intraspecific variation) within each isolate in both camel and local sheep.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization ofEchinococcus granulosusin livestock of Al-Madinah (Saudi Arabia)

2020

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Echinococcus granulosus is the causative agent of cystic echinococcosis, which has serious impacts on human and/or animal health, resulting in significant economic losses. Echinococcus granulosus comprises a number of intra-specific variants or strains at the genetic level. In Saudi Arabia, few studies were performed on genetic variations in Echinococcus species. Therefore, the present study aimed to investigate the phenotypic and genetic characterization of hydatid cysts harboured by sheep and camels in Al-Madinah Al-Munawarah. Samples of hydatid cysts were collected from local sheep (n = 25) and camels (n = 8). The morphological criteria of protoscoleces were investigated. To investigate the molecular characterization, random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR), single-stranded conformation polymorphism (SSCP) were carried out. DNA was extracted from individual fertile cysts and subjected to RAPD-PCR analysis (using five arbitrary primers) and PCR amplification of cytochrome c oxidase I (cox1) and 12S ribosomal ribonucleic acid (12S rRNA) genes. The PCR products were subjected to SSCP analysis for genetic discrimination in E. granulosus isolates. In addition, partially sequencing of the mitochondrial DNA cox1 genes was achieved for assessing the phylogenetic positions of collected isolates using some global published sequence data of cox1 genes. The rostellar hooks of camel and local sheep isolates show remarkable variability in their dimensions. Five distinct SSCP patterns were identified in the 12S rRNA gene, showing intraspecific variations in E. granulosus of camels and local sheep. Sequencing of (cox1) genes of both local sheep and camels exhibit high similarity with those of the same gene (E. granulosus sensu stricto) published in NCBI BLAST.

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“…La souche cameline (Soudan [17], Kenya [19,25], Lybie [21], Tunisie [14] et Niger [1,3], Mali [24], Mauritanie [15,16]) est caractérisée par la dominance des localisations pulmonaires. Par contre, la souche ovine est caractérisée par les localisations hépatiques (Tunisie et Maroc [13]). …”

Section: Discussionunclassified

“…granulosus semble proche de celle d'écrite dans certaines régions du Maghreb, en Afrique subsaharienne et au Moyen-Orient, où certaines études sur le typage moléculaire d'isolats des lignées G1 et G6 d'E. granulosus prouvent l'existence de diversités molé-culaires entre les isolats camelins et ovins (Tunisie, Algérie, Kenya et Soudan [11][12][13].…”

Section: Discussionunclassified

Étude des aspects lésionnels de l’échinococcose hydatique chez l’Homme en Mauritanie: fertilité, histologie des kystes hydatiques et viabilité des protoscolex

Salem

Schneegans²,

Chollet³

2010

Bull. Soc. Pathol. Exot.

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A lesional study on human hydatidosis (localization, fertility of cysts, protoscolex viability, and histological structure) focused on 63 human hydatid cysts from patients operated at CHN of Nouakchott was conducted during the period 1997-2007. The authors report the following results: the annual surgical incidence rate was in the order of 1.2% per 100,000 inhabitants. The lung was the primary localization of cyst (52%) followed by the liver (33%), spleen (4%), brain (3%), heart (2%), breasts (2%), kidney (2%), diaphragm (1%), and peritoneum (1%). The pulmonary cysts were larger and more fertile (76%) with protoscoleces also more viable (70%) compared with liver cysts (67-62%). The histology of hydatid cysts revealed in most cases a histological structure consistent with that described in the literature: thick cuticle, membrane proligeous, and capsules visible.

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Genetic differences between Tunisian camel and sheep strains of the cestodeEchinococcus granulosusrevealed by SSCP

Cited by 10 publications

References 28 publications

Infection of dogs with Echinococcus granulosus: causes and consequences in an hyperendemic area

Infection of dogs with Echinococcus granulosus: causes and consequences in an hyperendemic area

Molecular characterization ofEchinococcus granulosusin livestock of Al-Madinah (Saudi Arabia)

Étude des aspects lésionnels de l’échinococcose hydatique chez l’Homme en Mauritanie: fertilité, histologie des kystes hydatiques et viabilité des protoscolex

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