Genetic differences in the resistance of rats to isoprenaline-induced heart lesions

Mráz, M; E, Faltová; D, Lincová; Šedivý, J.; Gaier, Norbert; Mühlbachová, E; Ĉernohorský, M.; Vrána, A.

doi:10.1007/bf01907429

Cited by 10 publications

(5 citation statements)

References 15 publications

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“…Mráz et al (1995) demonstrated a significant increase in heart glycogen after daily injection of isoprenaline. Other papers (Faltová et al 1983a & b;Mráz et al 1986) demonstrated that a long administration of prolonged stressors like starvation also led to an increase of glycogen and decrease of cardiac damage. Glycogen certainly plays a positive and protective role for myocard.…”

Section: Discussionmentioning

confidence: 92%

“…Heart lesions after 1 mg ¡ kg ª1 of isoprenaline (accumulation index of 203 Hg) more less Mráz et al (1986) Heart lesions after water immersion restraint stress (accumulation index of 203 Hg) more less Starec et al (1994) Gastric ulcers after water immersion restraint stress (ulcer index) less more Starec et al (1994) Heart glycogen less more Mráz et al (1995) Severity of adjuvant arthritis less more Starec et al (1994) Open field -walking less more Rasková et al (1996) Open field -freezing more less Rasková et al (1996) Aggressive behaviour -intruder test less more Starec et al (1994) behavioural and pathophysiological differences observed were established repeatedly during different seasons of the year (Mráz et al 1995;Rasková et al 1996;Starec et al 1996).…”

Section: Observed Characteristics Is Ir Referencementioning

confidence: 99%

“…The content of 203 Hg in the heart was expressed as the accumulation index, i.e. the ratio of radioactivity found in 1 g of the heart to the radioactivity administered to 1 g of body weight (Mráz et al 1986).…”

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confidence: 99%

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The Influence of Repeated Prestressors on Single Stress Response in Rats

Yamamotová

Starec

̆ková

et al. 2000

Pharmacology & Toxicology

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The effect of various stressors of different intensity applied in random order before the final single immersion restraint stress was tested in two inbred rat strains, isoprenaline-sensitive and isoprenaline-resistant. The isoprenalinesensitive strain revealed higher incidence of heart lesions after this single acute stress and low incidence of gastric lesions. The isoprenaline-resistant strain had the opposite characteristics. These differences were constantly reproducible when this strong stressor was used. After prestress by different stressors (tail-flick, ether anaesthesia, Porsolt swimming stress) at different time schedules, the incidence of gastric ulcer lesions, the weight of organs (heart, adrenals, spleen) changed substantially in isoprenaline-sensitive rats only. The most important result was reversal of the extent of gastric lesions. The isoprenaline-sensitive strain revealed more lesions than the isoprenaline-resistant one. The repeated different prestressors mainly changed the reactivity of animals, isoprenaline-sensitive rats becoming more similar to isoprenaline-resistant rats. These findings urged us to interpret carefully the results obtained in stress research with different and multiple stressors both in animals and humans.

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Section: Discussionmentioning

confidence: 92%

Section: Observed Characteristics Is Ir Referencementioning

confidence: 99%

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The Influence of Repeated Prestressors on Single Stress Response in Rats

Yamamotová

Starec

̆ková

et al. 2000

Pharmacology & Toxicology

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“…In addition, energy metabolism differs considerably in animals with different degrees of resistance. For example, the glycogen content is much higher in the myocardium of rats with high resistance to isoproterenol than in rats with low resistance to it [14]. The NADH-oxidase pathway is the major source of reducing equivalents of the oxidation chain in LR rats, while in HR rats it is the succinate oxidase pathway [5].…”

Section: Comparison Of Lpo Parameters In Wistar Rats Differing In Resmentioning

confidence: 99%

Lipid peroxidation parameters in the internal organs of rats with different degrees of resistance to hypoxia

Khachatur'yan¹,

Gukasov²,

Komarov³

et al. 1996

Bull Exp Biol Med

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Lipid peroxidation and the antioxidant system of the heart, liver, and brain are studied in adult male Wistar rats with high and low resistance to hypoxia tested by "raising" to an altitude of 11.5 km and in intact outbred rats. These parameters are found to be the same in the brain of low-and high-resistance rats, while the brain content of lipid peroxidation products is higher in both groups of Wistar rats compared with outbred rats. The heart and liver parameters are coupled to the resistance to hypoxia. Antioxidant activity prevails over lipid peroxidation in the hearts and livers of high-resistance rats, confirming that oxidation plays a major role in the damaging and lethal effects of acute hypoxia.Key Words: lipid peroxidation; antioxidant system; hypoxia; low resistance; high resistance Hypoxic and reoxygenation damage are known to be associated with diminished activity of the antioxidant system (AOS) [12,13] and with activation of lipid peroxidation (LPO) [2,12,13]. Adaptation to hypoxia leads to changes in the activity of tissue AOS [3,8] and in the content of LPO products [6,7]. At the same time, species-specific and intraspecies (individual) variations of resistance to hypoxia have been demonstrated [1]. It can be assumed that the activity of tissue AOS and LPO is different in animals with different degrees of resistance to hypoxia. Meanwhile, few studies have been performed on rat blood [10], while the state of LPO and AOS in the vital organs in animals differing in their resistance to hypoxia has not been assessed. Our purpose was to investigate the LPO content and AOS activity in the brain, heart, and liver of rats with low and high resistance to hypoxia. MATERIALS AND METHODSExperiments were performed on 20 Wistar rats and 10 outbred rats in winter. Wistar rats were tested by "being raised" them to an altitude of 11.5 km during 1 min (twice, three weeks apart) [1]. The survival period of high-resistance (HR) rats was 5.3-fold longer in comparison with low-resistance (LR) rats (450+42 vs.86+5 sec, respectively, p<0.01). Three weeks after the repeat test, the brain, heart, and liver were rapidly excised under thiopental anesthesia after a 2.5-min perfusion with cold (0-4~ normal saline. The organs were homogenized and homogenates prepared from organs of 2-3 animals were pooled in one sample. There were 4-5 samples in each group of rats. Lipids were extracted from the homogenates as described elsewhere [11] with a chloroform-ethanol mixture (1:2) in the presence of 10 -s M ionol. Light absorbance (D) of lipids dissolved in hexane was measured in a Hewlett-Packard spectrophotometer at 215 nm (total lipids), 232 nm (diene conjugates), and 275 nm (ketotriene conjugates). All parameters were calculated per mg lipid. The oxidation indexes D232/215 and D275/215 reflecting the proportion of lipids oxi-0007..4888/96/0001-0022515.00 9Plenum Publishing Corporation

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“…For all measurements animals were killed by decapitation. Isoprenaline-sensitive rats (IS) react to application of isoprenaline with large myocardial lesions, whereas the rats resistant to isopr~naline (IR) have significantly smaller myocardial defects (Mraz et al 1986). Acute stress evokes heart pathology in the IS strain to the same extent as isoprenaline, but it induces gastric lesions in IR rats.…”

Section: Department Of Normal Pathological and Clinicalmentioning

confidence: 99%

Somatosensory Physiology

1998

The Journal of Physiology

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Chronic stress is known to affect the clinical symptoms of rheumatoid arthritis in humans, though the exact biochemical evidence is lacking. Under experimental conditions, partial food deprivation represents, aside from metabolic changes , emotional stress accompanied by chronic activation of the hypothalamo-pituitary-adrenocortical axis (Han et al. 1995).The model of chronic activation of hypothalamo-pituitaryadrenocortical axis by food restriction was used to determine whether stress-activated mechanisms might alleviate the development of adjuvant arthritis in rats. Four groups of rats (n = 3S) were used : intact control rats fed ad libitum (C), food -restricted rats fed 60% of C food intake (FR), arthritic rats fed ad libitum (AA) , and arthritic rats fed 60 % of AA food intake (AA-FR). Adjuvant arthritis was induced by the s.c. injection of dead Mycobacterium butyricum into the tails of male rats of the Long-Evans strain .Serum corticosterone (Cort) was elevated in the FR group (15'49 ± 2'1 flg 100 ml-1 vs. C, 5'02 ± O'OS flg 100 ml-1 , ANOVA, P< 0'01, mean ± S.E.M.). In the AA-FR group, no significant elevation of Cort was found. Enhanced hindpaw swelling (volumetric method) found in the AA group (2'37 ± 0'15 ml vs. C, 1'1 ± 0'05 ml, P< 0'01) was attenuated by stress. Serum albumin , as a negative acute phase reactant, was reduced in AA rats (23'9 ± 0'5 g 1-1 vs.C, 37'2 ± 0'05 g 1-1, P < 0'01) and chronic stress restored its levels (2S'1 ± 0'96 g 1-\ P< 0'01). The elevated level of serum N0 2 -/N0 3 -in AA rats (47' S ±5'7 flmoll-l vs. C, 11'3 ± 2'6 flmoll-l) was normalized in AA-FR animals (24'3 ± 3'9 flmoll-l) as well as the total antioxidative capacity in the spleen (O' S ± 0'04 mmoll-' vs. 0'66 ± 0'06 mmoll-1 ) . Higher pain-induced analgesia in AA (S'72 ± 0'34 s vs. C, 3'56 ± 0'21 s) rats as measured by tailflick was restored in the AA-FR group (6'OS ± 0'36 s).These results show an overall beneficial effect of chronic stress (food deprivation) for the development of adjuvant arthritis in rats fed ad libitum.Supported by Grant Agency of the Czech Republic -GACR 305/95/05712.

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Genetic differences in the resistance of rats to isoprenaline-induced heart lesions

Cited by 10 publications

References 15 publications

The Influence of Repeated Prestressors on Single Stress Response in Rats

The Influence of Repeated Prestressors on Single Stress Response in Rats

Lipid peroxidation parameters in the internal organs of rats with different degrees of resistance to hypoxia

Somatosensory Physiology

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