Genetic differentiation and hybrid identification using microsatellite markers in closely related wild species

Turchetto, Caroline; Segatto, Ana Lúcia Anversa; Beduschi, Júlia; Bonatto, Sandro L.; Freitas, Loreta B.

doi:10.1093/aobpla/plv084

Cited by 52 publications

(49 citation statements)

References 55 publications

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“…Each locus showed at least one private allele per population, which is in line with EST-SSR-based studies of Petunia species in Brazil [73] and Mangifera species in Australia [74].…”

Section: Genetic Diversity and Differentiationsupporting

confidence: 85%

Morphological and Genetic Diversity of Sea Buckthorn (Hippophae rhamnoides L.) in the Karakoram Mountains of Northern Pakistan

et al. 2018

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Sea buckthorn (Hippophae rhamnoides L.) is a dioecious, wind-pollinated shrub growing in Eurasia including the Karakoram Mountains of Pakistan (Gilgit-Baltistan territory). Contrary to the situation in other countries, in Pakistan this species is heavily underutilized. Moreover, a striking diversity of berry colors and shapes in Pakistan raises the question: which varieties might be more suitable for different national and international markets? Therefore, both morphological and genetic diversity of sea buckthorn were studied to characterize and evaluate the present variability, including hypothetically ongoing domestication processes. Overall, 300 sea buckthorn individuals were sampled from eight different populations and classified as wild and supposedly domesticated stands. Dendrometric, fruit and leaf morphometric traits were recorded. Twelve EST-SSRs (expressed sequence tags-simple sequence repeats) markers were used for genotyping. Significant differences in morphological traits were found across populations and between wild and village stands. A significant correlation was found between leaf area and altitude. Twenty-two color shades of berries and 20 dorsal and 15 ventral color shades of leaves were distinguished. Mean genetic diversity was comparatively high (He = 0.699). In total, three distinct genetic clusters were observed that corresponded to the populations’ geographic locations. Considering high allelic richness and genetic diversity, the Gilgit-Baltistan territory seems to be a promising source for selection of improved germplasm in sea buckthorn.

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“…Each locus showed at least one private allele per population, which is in line with EST-SSR-based studies of Petunia species in Brazil [73] and Mangifera species in Australia [74].…”

Section: Genetic Diversity and Differentiationsupporting

confidence: 85%

Morphological and Genetic Diversity of Sea Buckthorn (Hippophae rhamnoides L.) in the Karakoram Mountains of Northern Pakistan

et al. 2018

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“…Neighbour-joining tree of 126 individuals of petunia (coloured by taxon), constructed based on a matrix of microsatellite alleles shared among individuals (Turchetto et al 2015). Barik et al (2006) also found the genetic variation within the two species of Hibiscus and 16 varieties of Hibiscus rosa-sinensis L. through random amplified polymorphic (RAPD) markers.…”

Section: Figmentioning

confidence: 95%

“…Thirteen Microsatellites or SSR (simple sequence repeat markers) developed by Kimura et al (2009) in carnation by enriched genomic library method and its use for hybrid analysis. The parentage of two carnation mutant varieties obtained from intra-specific crosses and hybridity of the progenic Turchetto et al (2015) analysed two closely related species of Petunia exserta, which has a narrow endemic range and grows exclusively in rocky shelters, and Petunia axillaris, which was widely distributed and comprises three allopatric subspecies by EST-SSRs markers. Four major groups were identified in clustering analyses, which indicated that there are genetic distinctions among the groups (Fig.3).…”

Section: Figmentioning

confidence: 99%

Evaluation of Secondary Metabolites in Wheat Grain (Triticum Sp.) Grown in Humid South Eastern Plain Zone of Rajasthan (India)

Chachaiya¹,

Mehta²

2019

IJAEB

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Understanding the genetics of floricultural germplasm represents a value added component of managing collections. Molecular approaches collectively represent a potential goldmine of important information that can be applied to programmes of genetic improvement. The development and utilization of molecular markers for the exploitation and identification of plant genetic diversity is one of the most key developments in the field of molecular genetics studies. Molecular markers are popular tool, due to their stability, cost-effectiveness and ease of use for a variety of applications in the field of molecular genetics. To know the role of molecular markers in genetic studies of floricultural crops, a number of molecular marker techniques have been reviewed by many researchers in worldwide. However, all these reviews were meant for researchers with advanced knowledge of molecular markers and their utility in limited aspects under floricultural crops. This review presents an overview of different marker technologies and their variants with a comparative account of their characteristic features and applications especially diverse molecular techniques used in assessing plant genetic diversity, inter-relationship between the genotypes, genetic purity, DNA barcode, tagging of important genes, development of linkage maps, marker assisted selection and application of molecular markers in tissue culture of floricultural crops. Altogether, the characteristics and types of markers with their applications and some recent advancement in genetic studies of floricultural crops are discussed. Highlightsm Molecular markers play an important role for assessing the genetic diversity and identification of desirable traits, inter-relationship between the genotypes and genetic purity analysis. m Molecular markers can be helpful to develop a DNA barcode in flowering crops, tagging of qualitative genes, development of linkage maps, marker assisted selection and clonal fidelity analysis in tissue cultured flowering plants.

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“…Molecular markers involve amplification of specific amplified fragment length polymorphism (AFLP) [87], rapid amplification of polymorphic DNA (RAPD) [88] and single sequence repeat polymorphism (SSR) [89] markers related to fertility restoration and specific ribosomal DNA sequences. Molecular markers are the most reliable for identification of hybrids due to their unlimited number in the genome in comparison to chemical profiling which is time-consuming and limited in predicting hybrid ancestry [86].…”

Section: Selection Schemes For Sexual Hybridsmentioning

confidence: 99%

Plant Hybridization as an Alternative Technique in Plant Breeding Improvement

Mwangangi

Muli

Neondo

2019

AJRCS

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For ages, plant breeders have relied on the genetic variability that results from sexually crossing plants within the same species. However, the variability that exists within species populations is inadequate, hence the need to exploit desirable traits of interest in distantly related or even unrelated plants through hybridization techniques. Hybridization can be categorized into two; sexual and somatic. Sexual hybridization, also referred to as wide or distant hybridization involves combining two genomes from different parental taxa through pollination, either naturally or by induction. Somatic hybridization involves the fusion of somatic cells instead of gametes, which highly depends on the ability to obtain viable protoplasts and eventually differentiate them to whole plants in vitro. The impacts of hybrids can either be positive or negative. Among the positive attributes of hybrids that have been exploited is heterosis, which results either from dominance, over-dominance or epistasis. Negative ones include sterility, arrested growth of the pollen tube and embryo abortion. To overcome these problems, chromosome doubling, the use of hormones such as 2, 4-Dichlorophenoxyacetic acid (2, 4-D) and embryo rescue have been employed to overcome sterility, arrested growth of pollen tubes and embryo abortion respectively. After the development of hybrids, different hybrid identification techniques have been used to test them such as the use of molecular and morphological markers, cytogenetic analysis and fluorescent in situ hybridization. The use of hybridization techniques in plant improvement remains a vital tool to cross species barriers and utilization of important attributes in unrelated crop plants which could not have been achieved through conventional techniques of plant breeding.

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Genetic differentiation and hybrid identification using microsatellite markers in closely related wild species

Cited by 52 publications

References 55 publications

Morphological and Genetic Diversity of Sea Buckthorn (Hippophae rhamnoides L.) in the Karakoram Mountains of Northern Pakistan

Morphological and Genetic Diversity of Sea Buckthorn (Hippophae rhamnoides L.) in the Karakoram Mountains of Northern Pakistan

Evaluation of Secondary Metabolites in Wheat Grain (Triticum Sp.) Grown in Humid South Eastern Plain Zone of Rajasthan (India)

Plant Hybridization as an Alternative Technique in Plant Breeding Improvement

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