Genetic Dissection of Early Blight Resistance in Tetraploid Potato

Xue, Weiya; Haynes, Kathleen G.; Clarke, Christopher R.; Qu, Xinshun

doi:10.3389/fpls.2022.851538

Cited by 8 publications

(10 citation statements)

References 35 publications

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“…The fungicides in use are becoming less potent due to mutations in the active sites and fungicide-resistant populations of A. solani, which have been reported in multiple countries [8][9][10][11][12]. In addition, excess or prolonged application of fungicides may lead to the accumulation of these chemicals in the soil and water sources, which may become environmental contamination and food safety problems [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive transcriptome analysis of different potato cultivars provides insight into early blight disease caused by Alternaria solani

et al. 2023

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Background Early blight, caused by the necrotrophic fungal pathogen Alternaria solani, is an economically important disease affecting the tuber yield worldwide. The disease is mainly controlled by chemical plant protection agents. However, over-using these chemicals can lead to the evolution of resistant A. solani strains and is environmentally hazardous. Identifying genetic disease resistance factors is crucial for the sustainable management of early blight but little effort has been diverted in this direction. Therefore, we carried out transcriptome sequencing of the A. solani interaction with different potato cultivars with varying levels of early blight resistance to identify key host genes and pathways in a cultivar-specific manner. Results In this study, we have captured transcriptomes from three different potato cultivars with varying susceptibility to A. solani, namely Magnum Bonum, Désirée, and Kuras, at 18 and 36 h post-infection. We identified many differentially expressed genes (DEGs) between these cultivars, and the number of DEGs increased with susceptibility and infection time. There were 649 transcripts commonly expressed between the potato cultivars and time points, of which 627 and 22 were up- and down-regulated, respectively. Interestingly, overall the up-regulated DEGs were twice in number as compared to down-regulated ones in all the potato cultivars and time points, except Kuras at 36 h post-inoculation. In general, transcription factor families WRKY, ERF, bHLH, MYB, and C2H2 were highly enriched DEGs, of which a significant number were up-regulated. The majority of the key transcripts involved in the jasmonic acid and ethylene biosynthesis pathways were highly up-regulated. Many transcripts involved in the mevalonate (MVA) pathway, isoprenyl-PP, and terpene biosynthesis were also up-regulated across the potato cultivars and time points. Compared to Magnum Bonum and Désirée, multiple components of the photosynthesis machinery, starch biosynthesis and degradation pathway were down-regulated in the most susceptible potato cultivar, Kuras. Conclusions Transcriptome sequencing identified many differentially expressed genes and pathways, thereby contributing to the improved understanding of the interaction between the potato host and A. solani. The transcription factors identified are attractive targets for genetic modification to improve potato resistance against early blight. The results provide important insights into the molecular events at the early stages of disease development, help to shorten the knowledge gap, and support potato breeding programs for improved early blight disease resistance.

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Section: Introductionmentioning

confidence: 99%

Comprehensive transcriptome analysis of different potato cultivars provides insight into early blight disease caused by Alternaria solani

et al. 2023

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“…The resistance is suggested to be polygenic and thus quantitative in nature. It is also linked to the foliage maturity of potato cultivars [19,20,15]. It has been shown that potato cultivars maturing late are more resistant to early blight and vice versa [15,16,17,21,20].…”

Section: Introductionmentioning

confidence: 99%

“…The fungicides in use are becoming less potent due to mutations in the active sites and fungicide-resistant populations of A. solani, which have been reported in multiple countries [8,9,10,11,12]. In addition, excess or prolonged application of fungicides may lead to the accumulation of these chemicals in the soil and water sources, which may become environmental contamination and food safety problems [13,14,15].…”

Section: Introductionmentioning

confidence: 99%

“…Disease prevention based on resistant cultivars is the best long-term solution in potatoes for effectively managing early blight [15]. Even if no complete resistance has been identi ed, it is well known that potato cultivars have varying resistance levels against early blight.…”

Section: Introductionmentioning

confidence: 99%

“…It is also linked to the foliage maturity of potato cultivars [19,20,15]. It has been shown that potato cultivars maturing late are more resistant to early blight and vice versa [15,16,17,21,20]. Quantitative trait loci (QTLs) for foliar and tuber early blight resistance were identi ed on chromosomes 1, 5, 6, 7, 11, and 12, and chromosomes 1, 2, 3, 4, 8, 11, and 12, respectively, based on a tetraploid potato segregating population [17].…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive transcriptome analysis of different potato cultivars provides insight into early blight disease caused by Alternaria solani

Sivarajan

Abdelmeguid

Saripella

et al. 2022

Preprint

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Background Early blight disease in potatoes is one of the economically important diseases affecting the tuber yield and quality worldwide. It is caused by the necrotrophic fungal pathogen Alternaria solani. The disease is mainly controlled by chemical plant protection agents. However, the over-usage of these chemicals leads to the evolution of resistant A. solani strains and is environmentally hazardous. Therefore, identifying resistant potato cultivars or genetic disease resistance factors is the best strategy for the sustainable management of early blight. Results In this study, we have captured transcriptomes from three different potato cultivars with varying susceptibility to A. solani, Magnum Bonum, Désirée, and Kuras, at 18 and 36 hours post-infection. We identified many differentially expressed genes (DEGs) between these cultivars, and the number of DEGs increased with susceptibility and infection time. There were 649 transcripts commonly expressed between the potato cultivars and time points, of which 627 and 22 were up- and down-regulated, respectively. Interestingly, overall the up-regulated DEGs were twice in number as compared to down-regulated ones in all the potato cultivars and time points, except Kuras at 36 hours post-inoculation. In general, transcription factor families WRKY, ERF, bHLH, MYB, and C2H2 were highly enriched DEGs, of which a significant number were up-regulated. The majority of the key transcripts involved in the jasmonic acid and ethylene biosynthesis pathways were highly up-regulated. Many transcripts involved in the mevalonate (MVA) pathway, isoprenyl-PP, and terpene biosynthesis were also up-regulated across the potato cultivars and time points. Compared to Magnum Bonum and Désirée, multiple components of the photosynthesis machinery, starch biosynthesis and degradation pathway were down-regulated in the most susceptible potato cultivar, Kuras. Conclusions This study provides important insights into the molecular events occurring in early disease development and helps to shorten the knowledge gap and supports potato breeding programs for improved early blight disease resistance.

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