Genetic dissection of grain beta-glucan and amylose content in barley (Hordeum vulgare L.)

Islamovic, Emir; Obert, D. E.; Oliver, Rebekah E.; Harrison, Stephen A.; Ibrahim, Amir M. H.; Marshall, Juliet M.; Miclaus, Kelci; Hu, Gongshe; Jackson, Eric W.

doi:10.1007/s11032-012-9764-1

Cited by 42 publications

(38 citation statements)

References 38 publications

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“…If such an enzyme did exist, it would need to be present both in grasses and eudicots, given the ability of the Arabidopsis and N. benthamiana systems to synthesize MLG. Recent quantitative trait locus and genome-wide association studies (Islamovic et al, 2013;Shu and Rasmussen, 2014) suggest that other proteins should also be considered to play the role of Prt X in MLG synthesis (and/or regulation) and deserve further investigation.…”

Section: Toward a Model Of Mlg Assemblymentioning

confidence: 99%

Determining the Subcellular Location of Synthesis and Assembly of the Cell Wall Polysaccharide (1,3; 1,4)-β-d-Glucan in Grasses

Wilson

Lampugnani

et al. 2015

The Plant Cell

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The current dogma for cell wall polysaccharide biosynthesis is that cellulose (and callose) is synthesized at the plasma membrane (PM), whereas matrix phase polysaccharides are assembled in the Golgi apparatus. We provide evidence that (1,3;1,4)-b-D-glucan (mixed-linkage glucan [MLG]) does not conform to this paradigm. We show in various grass (Poaceae) species that MLG-specific antibody labeling is present in the wall but absent over Golgi, suggesting it is assembled at the PM. Antibodies to the MLG synthases, cellulose synthase-like F6 (CSLF6) and CSLH1, located CSLF6 to the endoplasmic reticulum, Golgi, secretory vesicles, and the PM and CSLH1 to the same locations apart from the PM. This pattern was recreated upon expression of VENUS-tagged barley (Hordeum vulgare) CSLF6 and CSLH1 in Nicotiana benthamiana leaves and, consistent with our biochemical analyses of native grass tissues, shown to be catalytically active with CSLF6 and CSLH1 in PM-enriched and PM-depleted membrane fractions, respectively. These data support a PM location for the synthesis of MLG by CSLF6, the predominant enzymatically active isoform. A model is proposed to guide future experimental approaches to dissect the molecular mechanism(s) of MLG assembly.

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Section: Toward a Model Of Mlg Assemblymentioning

confidence: 99%

Determining the Subcellular Location of Synthesis and Assembly of the Cell Wall Polysaccharide (1,3; 1,4)-β-d-Glucan in Grasses

Wilson

Lampugnani

et al. 2015

The Plant Cell

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“…This alone provided a resource to interrogate several thousand alleles in tandem and led to the construction of a high density consensus map defined by 2943 SNP loci ). The same study showed a orthologous relationship between barley and rice chromosomes, which can lead to gene discovery for various traits linked to specific QTL regions, such as beta glucan content in barley grain (Islamovic et al, 2013).…”

Section: Introductionmentioning

confidence: 83%

“…Comparison of QTL flanking markers, from Marquez-Cedillo et al study and our study, in barley integrated genetic maps at the GrainGenes website (http://wheat.pw.usda.gov) suggested that our QTL was in a different genetic location than the QTL from Marquez-Cedillo et al study. Localization of the QTL region in barley onto the rice annotated genome was enabled by flanking BOPA SNP sequences ) as described by Islamovic et al (2013). The candidate sdw1/denso gene (Jia et al, 2009) was identified (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The Falcon/Azhul mapping population developed by Islamovic et al (2013) to explore the genetics of beta glucan and amylose grain content was used in this study. Falcon (Helm et al, 1996) is a six-row hulless feed barley (Reg.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Harvested leaves from each line were placed into a 2.0-ml microcentrifuge tube, and ground into a fine powder using a sterilized test tube pestle and liquid nitrogen. DNA was extracted using a CTAB protocol as previously described in Islamovic et al (2013).…”

Section: Genotypingmentioning

confidence: 99%

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A new genetic linkage map of barley (Hordeum vulgare L.) facilitates genetic dissection of height and spike length and angle

Islamovic¹,

Obert²,

Oliver

et al. 2013

Field Crops Research

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a b s t r a c tPlant height and spike length and angle are important agronomic traits in the production of barley (Hordeum vulgare L.) due to strong correlations with lodging and disease. The objective of this study was to use QTL analysis to identify genetic regions associated with each trait in a recombinant inbred line (RIL) mapping population derived from a cross of Falcon by Azhul. Falcon is a spring six-row hulless feed barley with long spikes displaying obtuse angles, while Azhul is a spring dwarf, six-row hulless food barley with short spikes displaying acute angles. The population was genotyped using SNP, DArT and SSR markers and quantitative trait loci (QTL) were detected on chromosomes 2H (102.8 cM, spike length), 3H (89.2 cM, plant height and 38.2, spike angle and length), 4H (19.0 cM, spike length), and 5H (106.7 cM, spike angle). In conclusion, we developed a barley genetic map, which incorporated SNP, DArT and SSR markers, for detection of height and spike length and angle QTL. Three spike angle, one spike length and one plant height QTL were novel and by using comparative genomics we identified possible candidate genes involved in gibberellic acid signaling and auxin-and ethylene-responsive pathways. This knowledge can be used to generate suitable markers for barley breeding improvement. Published by Elsevier B.V.

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Breeding Naked Barley for Food, Feed, and Malt

Meints

Hayes

2019

Plant Breeding Reviews

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Genetic dissection of grain beta-glucan and amylose content in barley (Hordeum vulgare L.)

Cited by 42 publications

References 38 publications

Determining the Subcellular Location of Synthesis and Assembly of the Cell Wall Polysaccharide (1,3; 1,4)-β-d-Glucan in Grasses

Determining the Subcellular Location of Synthesis and Assembly of the Cell Wall Polysaccharide (1,3; 1,4)-β-d-Glucan in Grasses

A new genetic linkage map of barley (Hordeum vulgare L.) facilitates genetic dissection of height and spike length and angle

Breeding Naked Barley for Food, Feed, and Malt

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