Genetic distances and phylogenetic trees of different Awassi sheep populations based on DNA sequencing

Al-Atiyat, Raed M.; Aljumaah, Riyadh S.

doi:10.4238/2014.august.26.6

Cited by 7 publications

(5 citation statements)

References 17 publications

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“…Additionally, the admixture could be result of the high flow of genes between farms due to the practice to borrow or lease rams and lack of effective control in breeding programs [25] in Bulgaria. Similar results have been reported by Stahlberger-Saibetkova et al [7] and Al-Atiyat et al [32]. However, lower estimates of genetic differentiation between sheep breeds were found by Ocampo et al [25], Bozzi et al [5], Zhong et al [30], Álvarez et al [33], Ben Sassi et al [34], Hoda et al [14] and Abdelkader et al [35], who reported Fst ranging between 0.05 and 0.3.…”

Section: Population Genetic Diversitysupporting

confidence: 79%

Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markers

Mihailova

2021

Biotechnology & Biotechnological Equipment

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This study analyzed the genetic diversity and structure of two Bulgarian indigenous sheep (Ovis aries) breeds -Kotel and Teteven, each presented by 4 flocks, using 15 microsatellite markers. A total of 195 alleles were identified. Among the markers used, the most informative were INRA5 and OarFCB20 with PIC values of 0.87. Both breeds showed a high level of genetic diversity with average values of He and Ho, respectively 0.74 and 0.76. The observed high level of genetic diversity is combined with the absence of heterozygous deficiency reported as Fis values of 0.04 and 0.07, respectively for Kotel and Teteven breeds. Low level of genetic differentiation between the two breeds was observed, as evidenced by the low value of Fst of 0.09, and analysis of variance (ANOVA) data showing that only 9% of the total variation is due to variation between breeds, whereas 91% is due to variation within breeds. Furthermore, we revealed a breeding practice that comprises an exchange of animals between the breeds which reflects in the clustering obtained by STRUCTURE analysis. According to it, Kotel breed is a more homogenous population and forms cluster 1, whereas Teteven breed is more heterogenous and consists of two subpopulations that form two distinct clusters. The results obtained in the present study confirm the high potential of microsatellite markers in studies related to both breed affiliation and purity and can serve as a basis for the implementation of future management and conservation programs of these indigenous breeds.

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Section: Population Genetic Diversitysupporting

confidence: 79%

Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markers

Mihailova

2021

Biotechnology & Biotechnological Equipment

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“…Some recent studies have highlighted the genetic diversity of KSA sheep (Aljumaah et al, 2014 ; Adam et al, 2015 ). In addition, some studies have showed their differentiation from Jordan Awassi (Al-Atiyat and Aljumaah, 2014 ) and from Egypt and worldwide sheep (Peter et al, 2007 ). However, none of these studies provided information on the genetic structure of the local KSA sheep populations.…”

Section: Introductionmentioning

confidence: 99%

Microsatellite-Based Genetic Structure and Diversity of Local Arabian Sheep Breeds

et al. 2018

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The genetic diversity of the sheep breeds in the Arab countries might be considered to be a mirror of the ecology of the region. In this study, the genetic structure and diversity of sheep breeds from Saudi Arabia (Harri, Najdi, Naemi, Arb, and Rufidi) and Awassi sheep from Jordan as an out-group were investigated using 19 microsatellites. All the breeds had high intra-population genetic diversity expressed as allelic number (7.33) and richness (2.9) and, expected heterozygosity (0.77). Structure analysis revealed three main gene pools underlying the ancestral genetic diversity of the study populations. The first pool had Harri, Najdi, and Rufidi breeds; the second had Naemi and Awassi breeds, and the third had the Arb breed which was significantly differentiated from the other breeds. Factorial correspondence analysis lent further support to the presence of the three gene pools. Although the outgroup Awassi sheep was more clearly differentiated, it still genetically close to Naemi sheep. The differentiation of the Arb breed could have been resulted from geographic and reproductive isolation. On the other hand, the genetic structure of the other two gene pools could be the result of the past and recent gene flow between individuals reared in the region known to be the center for animal husbandry and trading until the current time.

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“…However, most of its available sequences were too short to be included in this study, resulting in its low number of samples inside our dataset, the majority of which come from Pakistan. We hypothesize that this breed, which is largely spread in the Near East, could have some ecotypes [ 47 ] connected to Egyptian breeds, as found by Ahbara et al [ 11 ] through their 50K SNP BeadChip analyses, and more specifically to Rahmani and Barki [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Shedding Light on the Origin of Egyptian Sheep Breeds by Evolutionary Comparison of Mitochondrial D-Loop

Germot

Khodary

Othman³

et al. 2022

Animals

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(1) Background: It has been recognized that the origin of fat-tailed sheep occurred within coarse wool breeds and that this character was introgressed several times into thin-tailed populations. However, no study has investigated this idea for Egyptian breeds using mtDNA analyses. (2) Methods: Using new sequences of the control region, we constructed a database of 467 sequences representing 37 breeds including fat- and thin-tailed ones with 80 Egyptian individuals belonging to six local breeds (Barki, Fallahi, Ossimi, Rahmani, Saidi, Sohagi). The phylogenetic tree obtained with the maximum likelihood method was submitted to the Newick Extra program to count the direct and indirect links between the individuals of each breed. (3) Results: Several Egyptian breeds were strongly connected to “primitive” thin-tailed breeds from Europe, indicating a clear genetic background of the “thin tail” breed type that supports the view of archeologists. In several cases, we suspected Western Asian breeds to be involved in the introgression of the fat tail character. In contrast, the Ossimi breed showed a high affinity to a fat-tailed breed of Western Asia, suggesting a direct migration and no thin tail ancestors. The Saidi is unique as our analyses revealed its strong connection with thin-tailed Sudanese breeds.

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Genetic distances and phylogenetic trees of different Awassi sheep populations based on DNA sequencing

Cited by 7 publications

References 17 publications

Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markers

Genetic diversity and structure of 2 indigenous sheep breeds (Kotel and Teteven) in Bulgaria using microsatellite markers

Microsatellite-Based Genetic Structure and Diversity of Local Arabian Sheep Breeds

Shedding Light on the Origin of Egyptian Sheep Breeds by Evolutionary Comparison of Mitochondrial D-Loop

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