The present study was carried out in Agricultural Research Station, Hagari during rabi 2020, it was undertaken to assess nature of genetic variability and diversity among 200 mutant lines of M4 generation. The study revealed wide variation for yield and yield attributing traits, moderate GCV and PCV coupled with high heritability and genetic advance was observed for stem girth, panicle length and panicle width. The traits viz., stem girth, panicle length, panicle weight, panicle width, 100-seed weight, grain yield per plant and fodder yield showed high heritability and genetic advance as per cent of mean. A wider genetic diversity was observed for the different traits studied among the mutant lines as evidenced by the formation of thirteen clusters for the 200 mutant lines. Out of thirteen characters studied, fodder yield contributed majorly towards divergence with value of 37.35 per cent followed by 100-seed weight contributes (20%), grain yield contributes (15%) and other traits contribute minorly for divergence.