Genetic divergences of South and Southeast Asian frogs: a case study of several taxa based on 16S ribosomal RNA gene data with notes on the generic name Fejervarya

Hasan, Mahmudul; ISLAM, Mohammed Mafizul; KHAN, Md. Mukhlesur Rahman; Igawa, Takeshi; ALAM, Mohammad Shafiqul; Tjong, Djong Hon; KURNIAWAN, Nia; JOSHY, Hareesh; SEN, Yong Hoi; BELABUT, Daicus M.; KURAMOTO, Mitsuru; SUMIDA, Masayuki

doi:10.3906/zoo-1308-36

Cited by 32 publications

(33 citation statements)

References 52 publications

(72 reference statements)

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“…Moreover, the lack of divergence time estimations in those previous studies prevents us from deep understandings of divergence history in M. okinavensis and its relatives. Using much shorter mitochondrial DNA sequence data deposited in the DNA Data Bank of Japan (DDBJ), Hasan et al () conducted phylogenetic analyses for species and populations of Microhyla including M. mixtura , and the Yaeyama, Okinawa and Amami populations of M. okinavensis (each represented only by a single sample). They suggested closer affinity of the Yaeyama population of M. okinavensis with M. mixtura than with the Okinawa and Amami populations of M. okinavensis .…”

Section: Introductionmentioning

confidence: 99%

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Relict distribution of Microhyla (Amphibia: Microhylidae) in the Ryukyu Archipelago: High diversity in East Asia maintained by insularization

et al. 2019

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The Ryukyu Archipelago, located at the southwestern part of Japan, is known as a group of continental islands and harbours many endemic taxa, supposedly reflecting its fairly long isolation from the Eurasian continent, Taiwan and the Japanese main islands. Microhyla okinavensis has been known as an endemic member of the terrestrial fauna of this archipelago. Molecular phylogenetic analyses using samples from nearly all island populations of the species and representative samples of other east Asian congeneric species revealed that M. okinavensis consists of four distinct subclades, of which the Amami, Okinawa and Miyako subclades, though exhibiting distinct genetic differentiations from each other, formed a monophyletic group (clade A). The remaining Yaeyama subclade was exclusively sister to M. mixtura from inland China, forming another monophyletic group (clade B), rendering M. okinavensis in the current definition paraphyletic. These results, as well as estimated dates of divergence from related taxa, indicate that M. okinavensis actually includes more than one distinct species. The results indicate that M. okinavensis and M. mixtura are relict species with disjunct distributions which had been most probably caused by invasion of M. fissipes in intervening areas.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Relict distribution of Microhyla (Amphibia: Microhylidae) in the Ryukyu Archipelago: High diversity in East Asia maintained by insularization

et al. 2019

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“…From our comprehensive samples, we found cryptic diversity within Asian common treefrogs (P. leucomystax complex) in Thailand. Although the integrated taxonomy has been suggested for identification, there are limitations in practice because they are geographically widespread species (Hasan et al, 2014). We used the phylogenetic analyses and genetic differences in 16S, it provided reliable results in species identification of P. leucomystax complex, as in previous molecular studies of morphologically cryptic amphibians (Lehtinen et al, 2007…”

Section: Cryptic Diversity Of P Leucomystax Complex In Thailandmentioning

confidence: 99%

“…Thus, it contributes to the diversity of ecosystems and organisms including amphibians (Bain and Hurley, 2011;Hertwig et al, 2012). Although new amphibian species in this region have been continuously reported (Köhler et al, 2005;Hasan et al, 2014), their diversity remains underestimated due to the prevalence of cryptic species (sibling species or species complexes) (Stuart et al, 2006;Hasan et al, 2014). "Cryptic species" refers to more than one distinct species that were classified as the same species due to morphological similarity (Bickford et al, 2007;Trontelj and Fišer, 2009).…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of the morphologically cryptic Asian common treefrogs (Anura: Rhacophoridae, Polypedates leucomystax complex) in Thailand

2020

ANRES

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New amphibian species in Southeast Asia have been continuously reported. However, the diversity of amphibians in this region is underestimated due to the prevalence of cryptic species. Recent molecular studies revealed that many amphibian species in Southeast Asia including Polypedates leucomystax comprised cryptic species. In this study, we reveal the cryptic diversity within P. leucomystax complex in Thailand. The 16S rRNA gene (588 to 621 bp) was analyzed from 218 specimens in 54 provinces of Thailand. Then, a different region of 16S (376 to 379 bp) was analyzed using 19 selected sequences from the first analyses and six tadpole from GenBank. Based on phylogenetic analyses, the first tree showed P. leucomystax complex was a monophyletic group. Our specimen collection fell within four clades; A-clade (P. leucomystax), B-clade (Polypedates sp.), C-clade (P. megacephalus) and E-clade (P. discantus). The most intraspecific p-distances were less than or equal to 0.03 while the interspecific p-distance were greater than 0.03. Moreover, the second phylogenetic analysis showed that six tadpole sequences were clustered in the clades of P. cf. mutus1, P. braueri and Polypedates sp. We found P. megacephalus and P. leucomystax were the dominant species in Indochinese and Sundaic subregions, respectively. Furthermore, the B-clade could be considered as a new clade because their genetics differred from the others. Our results concluded that there was cryptic diversity within Asian common treefrogs in Thailand. Molecular identification using 16S marker in our study provided reliable results to identify this cryptic species and will be useful for further studies in population genetics and biogeography.

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“…There were some researchers about amphibians in West Sumatra including L. blythii both aspects of taxonomy, evolution, ecology, and genetics of the specific type. As has been done by [5]- [6]- [7]- [8]- [9] however the evolutionary relationships among its family to interpret biogeographic classification is very need to be analyzed. L. blythii population has been decreased.…”

Section: Introductionmentioning

confidence: 99%

Morphological Variation of Limnonectes blythii in West Sumatera, Indonesia

Hendri

Tjong

Dahelmi

et al. 2018

int.jour.sci.res.mana

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Genetic divergences of South and Southeast Asian frogs: a case study of several taxa based on 16S ribosomal RNA gene data with notes on the generic name Fejervarya

Cited by 32 publications

References 52 publications

Relict distribution of Microhyla (Amphibia: Microhylidae) in the Ryukyu Archipelago: High diversity in East Asia maintained by insularization

Relict distribution of Microhyla (Amphibia: Microhylidae) in the Ryukyu Archipelago: High diversity in East Asia maintained by insularization

Molecular identification of the morphologically cryptic Asian common treefrogs (Anura: Rhacophoridae, Polypedates leucomystax complex) in Thailand

Morphological Variation of Limnonectes blythii in West Sumatera, Indonesia

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