Genetic Diversity among Clinical Isolates of
            <i>Acremonium strictum</i>
            Determined during an Investigation of a Fatal Mycosis

Novicki, Thomas J.; LaFe, Karen; Bui, Lynda; Bui, Uyen; Geise, Robert; Marr, Kieren A.; Cookson, Brad T.

doi:10.1128/jcm.41.6.2623-2628.2003

Cited by 36 publications

(33 citation statements)

References 27 publications

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“…Since there are close morphological similarities between A. strictum and A. kiliense, it is possible that these cases were actually caused by the latter species or by some other species of the genus. In this context, attention may be drawn to two recent case reports (9,27) where sequenced isolates identified as A. strictum in fact belonged to the Acremonium sclerotigenum-Acremonium egyptiacum group (30). In an attempt to differentiate A. kiliense from A. strictum, Perdomo et al (30) observed that isolates of A. kiliense form unicellular chlamydospores and adelophialides (reduced forms of phialides without a basal septum) in the vegetative or substrate hyphae (not in aerial hyphae) when grown on oatmeal agar at 24°C for about 2 weeks.…”

Section: Case Reportmentioning

confidence: 99%

Acremonium kiliense: Reappraisal of Its Clinical Significance

et al. 2011

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A case of Acremonium kiliense peritonitis is described. Diagnosis was established by repeated isolation of the fungus from peritoneal dialysate and by its identification on the basis of morphological characteristics and sequencing of internal transcribed spacer (ITS) regions of ribosomal DNA (rDNA). This report and available literature suggest that A. kiliense may have a greater clinical significance than hitherto recognized. CASE REPORTA 75-year-old Jordanian man with a long history of diabetes mellitus and hypertension developed end-stage kidney disease requiring continuous ambulatory peritoneal dialysis (CAPD). His course of CAPD was largely uneventful except for a single episode of bacterial peritonitis, for which he was hospitalized and treated with parenteral antibiotics, with an excellent response. In January 2010, he became febrile, with clinical evidence of peritonitis, and was admitted to the hospital. The peritoneal fluid was turbid, with a white blood cell (WBC) count of 2.1 ϫ 10 9 /liter. On initial microscopic examination, the presence of fungal elements in the peritoneal dialysate was missed. The peritoneal fluid sample was inoculated into aerobic Bactec blood culture bottles, which yielded a growth after 59 h of incubation. The Gram-stained smear from blood culture bottles showed branched hyphal elements. On Sabouraud dextrose agar (SDA; Oxoid Ltd., Basingstoke, England), the specimen yielded slimy colonies with a pinkish appearance ( Fig. 1). Microscopic examination of the primary culture (isolate Kw441-2010) showed hyaline hyphae, with scanty sporulation. A provisional identification of Acremonium/Fusarium species was made, and the growth was subcultured on Sabouraud dextrose agar and oatmeal agar (OMA; oatmeal [30 g], agar [20 g], distilled water [1 liter]) for further identification and antifungal susceptibility testing. Subsequent cultures of the peritoneal fluid yielded the same fungus on three occasions. A serum sample was obtained for the detection of galactomannan (Platelia Aspergillus enzyme immunoassay [EIA] kit; BioRad, Marnes-la-Coquette, France) and (1-3)-␤-D-glucan (Fungitell; Associates of Cape Cod); the latter test was positive (253 pg/ml). An Etest performed on RPMI 1640 medium supplemented with 2% glucose revealed that the isolate was resistant to amphotericin B and caspofungin but susceptible to voriconazole and posaconazole, with MIC values of Ͼ32 g/ml, Ͼ32 g/ml, 0.064 g/ml, and 0.75 g/ml, respectively. The patient was started on voriconazole, with a loading dose of 400 mg, followed by a maintenance dose of 200 mg, given every 12 h via the oral route. Although the patient showed clinical improvement after 2 weeks of voriconazole therapy, the peritoneal dialysate remained turbid (WBC counts, 2.0 ϫ 10 9 / liter). Abdominal ultrasound examination did not reveal any evidence of intraperitoneal adhesions or organ invasion. Since the response to treatment was not adequate, the Tenckhoff catheter was removed and the patient was temporarily switched to hemodialysis. After 1 week of a...

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Section: Case Reportmentioning

confidence: 99%

Acremonium kiliense: Reappraisal of Its Clinical Significance

et al. 2011

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“…Eight KT isolates (i.e., K5217-2-2, K5217-2-3, K5225-6-1, K5225-13-3, K5225-14-3, K5225-15-1, K5225-19-1, and K5229-19-2) were placed in the cluster represented by Acremonium strictum (Nectriaceae), including the ex-type strain CBS 346.70 (isolated from Triticum aestivum leaf) of A. strictum (AY138483; see Table 3 in Novicki et al 2003) and A. strictum AY 138484 and AY 138485 (isolated from human blood; Novicki et al 2003). Although cultural and morphological characteristics of these KT isolates agreed with those of A. strictum (Gams 1971;Domsch et al 2007), additional molecular phylogenetic data are needed to identify KT isolates to species.…”

Section: Resultsmentioning

confidence: 99%

Mycobiota of the Takamatsuzuka and Kitora Tumuli in Japan, focusing on the molecular phylogenetic diversity of Fusarium and Trichoderma

Kiyuna¹,

An²,

Kigawa³

et al. 2008

Mycoscience

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“…A. strictum CBS 102295 was not identified, and this might be due to genetic diversity among isolates of the species. Novicki et al (39) found a greater-than-expected variation among strains previously identified as A. strictum. In sequence analyses of the ITS regions and the D1/D2 variable domain of the 28S ribosomal DNA, five clinical isolates phenotypically identified as A. strictum were found to have Ͻ99% similarities to the A. strictum type strain (CBS 346.70) at the ITS and 28S loci.…”

Section: Discussionmentioning

confidence: 99%

Identification of Medically Important Molds by an Oligonucleotide Array

Hsiao

Huang

Bouchara³

et al. 2005

J Clin Microbiol

108

100

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Infections caused by fungi have increased in recent years. Accurate and rapid identification of fungal pathogens is important for appropriate treatment with antifungal agents. On the basis of the internal transcribed spacer 1 (ITS 1) and ITS 2 sequences of the rRNA genes, an oligonucleotide array was developed to identify 64 species (32 genera) of clinically important filamentous (or dimorphic) fungi. These 64 species included fungi causing superficial, cutaneous, subcutaneous, and invasive infections. The method consisted of PCR amplification of the ITS regions using a pair of universal primers, followed by hybridization of the digoxigenin-labeled PCR products to a panel of species-or group-specific oligonucleotides immobilized on a nylon membrane. Of 397 fungal strains (290 target and 107 nontarget strains) tested, the sensitivity and specificity of the array was 98.3% (285/290) and 98.1% (105/107), respectively. Misidentified strains were usually those belonging to the same genus of the target species or having partial homology with oligonucleotide probes on the membrane. The whole procedure can be finished within 24 h starting from isolated colonies; reproductive structures, which are essential for the conventional identification methods, are not needed. In conclusion, the present array is a powerful tool for identification of clinically important filamentous fungi and may have the potential to be continually extended by adding further oligonucleotides to the array without significantly increasing the cost or complexity.

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Genetic Diversity among Clinical Isolates of Acremonium strictum Determined during an Investigation of a Fatal Mycosis

Cited by 36 publications

References 27 publications

Acremonium kiliense: Reappraisal of Its Clinical Significance

Acremonium kiliense: Reappraisal of Its Clinical Significance

Mycobiota of the Takamatsuzuka and Kitora Tumuli in Japan, focusing on the molecular phylogenetic diversity of Fusarium and Trichoderma

Identification of Medically Important Molds by an Oligonucleotide Array

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