Genetic Diversity Among Three Cultivars Of Peanut (Arachis hypogaea L.) Based On Rapd Markers

Suryadi, Suryadi; Yuniaty, Alice; Susanto, Agus Hery

doi:10.20884/1.bioe.2019.1.2.1709

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…Primer OPB-2 showed 75% polymorphic bands with lengths of 400-1300 bp that were specific to the said primer only (Figure 4). However, the results of this study were better than those of the work by Suryadi et al (2019), who failed to obtain any polymorphic pattern for three tested peanut cultivars despite amplifying two bands.…”

Section: Rapd Profiles Of Cinchonacontrasting

confidence: 85%

Genetic profiles of three Cinchona species in Junghuhn Natural Reserve, Indonesia

Sasongko¹,

Yuniati²,

Billmora³

2021

SABRAOJBG

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Cinchona species were widely used as ancient medicines for different diseases because they contain the active component quinine and its derivatives. However, studies on the molecular aspects of cinchona, including its genetic diversity, have not been reported because most previous works focused on the administration of the antimalarial cinchona alkaloid. Quinine is also being tested as alternative compound for the treatment of Covid-19. The Junghuhn Natural Reserve in Indonesia contains three different types of cinchona plants, namely, Cinchona calisaya, Cinchona pubescens, and Cinchona sp. Given that the genetic diversity and kinship of these species have never been studied, collecting data on the cinchona gene pool has become imperative. This study analyzed the genetic diversity of the cinchona species in the Junghuhn Natural Reserve, Indonesia, by using eight RAPD markers, i.e., OPA-2, OPA-9, OPB-02, OPB-03, OPB-04, OPB-05, OPB-7, and OPJ-07, during 2020 at the University of Jenderal Soedirman, Purwokerto-Indonesia. Polymorphic band data were obtained. Then, phenogram analysis was conducted by using UPGMA and maximum parsimony with MEGA7. The RAPD profiles of Cinchona species (C. calisaya, C. pubescent, and Cinchona sp.) revealed polymorphism with different markers, i.e., OPA-2 (90%), OPB-2 (75%), OPB-5 (75%), OPB-3 (66.66%), OPB-4 (66.66%), OPB-7 (66.66%), OPJ-7 (66.66%), and OPA-9 (58.33%) sequentially with total polymorphism (70.62%). C. calisaya was identified as the most distinctive species. UPGMA yielded a coefficient of 0.200 and two distinctive groups: Group I, which comprised C. pubescens and Cinchona sp. with the p-distance value of 0.333, and Group II, which contained C. calisaya. Ixora sp. was treated as an outgroup plant. The topology of the dendrogram was consistent with that of the UPGMA dendrogram. Results may be used for the further exploration of the genetic diversity of cinchona species.

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Section: Rapd Profiles Of Cinchonacontrasting

confidence: 85%

Genetic profiles of three Cinchona species in Junghuhn Natural Reserve, Indonesia

Sasongko¹,

Yuniati²,

Billmora³

2021

SABRAOJBG

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“…Therefore, molecular markers are beneficial complements for morphological and isozyme properties because they are not influenced by the environment (Ramesh et al 2020). Several PCR-based molecular markers have been developed to assist genetic diversity studies (Suryadi et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of shallots (Allium ascalonicum L.) from several locations in North Sumatra, Indonesia based on RAPD markers

et al. 2022

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Abstract. Hasanah Y, Mawarni L, Hanu, H, Lestami A. 2022. Genetic diversity of shallots (Allium ascalonicum L.) from several locations in North Sumatra, Indonesia based on RAPD markers. Biodiversitas 23: 2405-2410. Shallot (Allium ascalonicum L.) is one of the leading horticultural and spice commodities in Indonesia. Assembling shallot varieties to get a good seed quality requires a high genetic diversity which can be analyzed using molecular markers. This study aims to identify the genetic diversity of shallots from several locations in North Sumatra, Indonesia based on Random Amplified Polymorphic DNA (RAPD) markers. This research was conducted at the Biotechnology Laboratory, Faculty of Agriculture, University of Sumatera Utara, Medan from September to October 2021. A total of 11 shallot varieties from several locations in North Sumatra were genetically analyzed based on RAPD markers with OPA-13, OPB-07, OPD-20, OPM-01 primers. The results showed that 11 shallot varieties originating from several locations in North Sumatra had a high genetic diversity with the presence of two main groups at a dissimilarity coefficient of 76%. These beneficial results can be used as a complement to morphological markers in the genetic study of shallots for breeders to decide what genotypes will be crossed to make new genetic combinations in the development of shallots. This study reports the success of the shallot varieties’ fingerprints using RAPD markers.

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Genetic Diversity Among Three Cultivars Of Peanut (Arachis hypogaea L.) Based On Rapd Markers

Cited by 2 publications

References 8 publications

Genetic profiles of three Cinchona species in Junghuhn Natural Reserve, Indonesia

Genetic profiles of three Cinchona species in Junghuhn Natural Reserve, Indonesia

Genetic diversity of shallots (Allium ascalonicum L.) from several locations in North Sumatra, Indonesia based on RAPD markers

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