Genetic diversity among Toxoplasma gondii isolates from different hosts and geographical locations revealed by analysis of ROP13 gene sequences

Py, Wang; Lu, Peng; Xu, MJ; Li, J; Zhou, D.; Yang, Jian; Ni, Z. L.; Yuan, ZG; Lin, Rui

doi:10.5897/ajb12.056

Afr. J. Biotechnol.

2012

DOI: 10.5897/ajb12.056

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Genetic diversity among Toxoplasma gondii isolates from different hosts and geographical locations revealed by analysis of ROP13 gene sequences

Wang Py¹,

Peng Lu²,

MJ Xu³

et al.

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Cited by 2 publications

References 15 publications

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Toxoplasma gondii rhoptry protein 38 gene: sequence variation among isolates from different hosts and geographical locations

Zhang

Chen

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Toxoplasma gondii is an obligate intracellular parasite that is able to infect almost all mammalian species, and may lead to toxoplasmosis of the host. In the present study, we examined sequence variation in rhoptry protein 38 (ROP38) genes among T. gondii isolates collected from different hosts and geographical regions. The complete ROP38 gene from 13 T. gondii isolates was amplified and sequenced. The results of sequence alignments showed that the lengths of the entire ROP38 gene ranged from 2646 to 2650 bp, with a sequence variation of 0.2-1.1%, among the 13 T. gondii isolates. This result indicated low sequence variation in the ROP38 gene. Phylogenetic analysis of ROP38 sequences using Bayesian inference showed that the clustering of the 13 T. gondii isolates was not consistent with their respective genotypes. This result indicates that the ROP38 gene is not a suitable genetic marker for population genetic studies of different T. gondii genotypes from different hosts and geographical locations, but may represent a potential vaccine candidate against toxoplasmosis, and hence worthy of further research.

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Toxoplasma gondii rhoptry protein 38 gene: sequence variation among isolates from different hosts and geographical locations

Zhang

Chen

et al. 2014

Genet. Mol. Res.

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Sequence variation in the Toxoplasma gondii ROP20 gene among strains from different hosts and geographical locations

et al. 2015

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ABSTRACT. Toxoplasma gondii, an opportunistic protozoan parasite, infects almost all warm-blooded animals. In this study, we examined the sequence variation in rhoptry protein 20 (ROP20) genes among 18 T. gondii isolates collected from different hosts and geographical regions. Full length ROP20 genes were amplified and sequenced. The results showed that the genes were 1659 bp in length and contained only a single exon, and that the A+T content varied from 46.68 to 47.20% 8415 Toxoplasma gondii ROP20 sequence variation ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 8414-8419 (2015) among the 18 strains. The results of sequence alignment indicated that there were 30 variable nucleotide positions (0-1.40%) in the 18 T. gondii strains containing 18 transitions and 11 transversions, representing 1.81% overall sequence variation. Phylogenetic analysis of the ROP20 sequences showed that ROP20 variation could differentiate between the clonal lineage genotypes I and ToxoDB #9, indicating that ROP20 exhibits a relatively marked degree of sequence diversity and might represent a novel genetic marker for intraspecies phylogenetic analyses of T. gondii.

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Genetic diversity among Toxoplasma gondii isolates from different hosts and geographical locations revealed by analysis of ROP13 gene sequences

Cited by 2 publications

References 15 publications

Toxoplasma gondii rhoptry protein 38 gene: sequence variation among isolates from different hosts and geographical locations

Toxoplasma gondii rhoptry protein 38 gene: sequence variation among isolates from different hosts and geographical locations

Sequence variation in the Toxoplasma gondii ROP20 gene among strains from different hosts and geographical locations

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