Genetic diversity analysis of drought tolerant flax varieties based on genome-wide SNP

Abstract

To clarify the genetic diversity among typical drought tolerant flax varieties, in this study, the Single-nucleotide polymorphism sites (SNPs) of 29 drought tolerant flax varieties were identified by whole-genome resequencing, and their SNP characteristics and genetic relationships were analyzed. The results showed that 1069106 SNP sites were obtained from the genome of the 29 drought tolerant flax varieties, among which the variety with the largest number of SNP was Zhangya 2 (1027658) and the least was Shache flax (801300). Among the types of SNP variation that occurred, the number of transitions (671103) was higher than transversions(398003). Phylogenetic analysis divided all 29 flax varieties into three groups, each with a wide range of sources, and some flax originating from the same region were located in the same group, indicating a rich genetic diversity of these 29 flax varieties. PCA (Principal component analysis) also divided all the flax varieties into three groups, which showed similarity to the phylogenetic tree grouping, which provides evidence for the results of the phylogenetic analysis. Among them, five varieties, Linxiabai, Gaolanbai, Lingtaizhuangna, Shache flax and Shache precocious red, showed evidently close genetic relationship both in phylogenetic analysis and PCA. This study can prove that the 29 drought tolerant flax varieties have rich genetic diversity, which lays the foundation for the genetic breeding of drought tolerant flax, the development of specific molecular markers, and the identification of germplasm resources.

Analyzing the Diversity of MYB Family Response Strategies to Drought Stress in Different Flax Varieties Based on Transcriptome Data

“…In our previous studies, we confirmed that there is a wide genetic diversity in the genomes of different varieties of flax, which is influenced by many factors, such as parental and geographical origin [37]. In this study, we confirmed that the distribution of DEGs in the MYB family of different flax varieties is also significantly different under drought stress (Table 1, Figure 1).…”

“…Based on previous research, such as the identification and cluster analysis of drought resistance in early field experiments, combined with the recommendations of drought-resistant varieties from the national oil crop industry technical system, 18 drought-resistant flax varieties and two water-sensitive varieties as control varieties were screened in this study to conduct the drought stress test ( Table 4 , Supplementary Table S4 ) [ 37 ].…”