Genetic Diversity Analysis of Lates calcarifer (Bloch 1790) in Captive and Wild Populations Using RAPD Markers

Abstract

Lates calcarifer (Bloch 1790) is one of the major economically important cultivable fish species in India. In this study, three populations of L. calcarifer was selected to assess the genetic diversity. Of which, two wild (Mudaslodai, Muthupettai) and one captive (Mutukadu) population. The genetic diversity of three populations of this species was studied using Random Amplified Polymorphic DNA (RAPD) markers. Ten random primers were used for the assessment of their genetic diversity and construction of the dendrogram. A total of 589 scorable bands were obtained, 93.12% of them were polymorphic. The Nei's gene diversity (H) of two wild populations were more (0.0504 ± 0.0670 and 0.0519 ± 0.0953) than the captive population (0.0489 ± 0.0850). The clustering pattern obtained by UPGMA method emphasized the wild populations were clustered in one clade and captive population was deviated into another clade. This study proved that RAPD analysis has the ability to discriminate L. calcarifer populations. Further molecular studies, comprising a higher number of molecular tools are still required to precisely evaluate the genetic structure of all seabass populations along the Indian coast.

Morphometric and Genetic Variation in Three Populations of Indian Salmon (Polydactylus plebeius)

“…In this dendrogram Pazhayar and Vellar populations are closely related than Cuddalore population. (Shifat et al, 2003;Rahman et al, 2009;Chandra et al, 2010;Mehrotra et al, 2010;Rajasekar et al, 2012).…”

“…Shifat et al (2003) have also reported RAPD Jaccards dissimilarity coefficient in 34 individuals of Tenualosa ilisha and observed 20.41% polymorphism. Rajasekar et al (2012) investigated that the RAPD profile showed 93.12% polymorphic loci among the three populations of Lates calcarifer. The present study inferred from these RAPD data that showed relatively high level (92.10%) of genetic polymorphism and is possibly due to the small sampling size.…”

Pengelolaan Sumberdaya Ikan Kakap Putih (Lates calcarifer, Bloch, 1790) Berdasarkan Karakter DNA Mitokondria di Perairan Pesisir Kabupaten Merauke

“…Gen Sitokrom Oksidase Subunit I (COI) banyak digunakan dalam berbagai studi, seperti: populasi genetik (Dailami et al, 2021;Saleky et al, 2016), identifikasi spesies dan studi keanekaragaman hayati (Martsikalis et al, 2014;Thu et al, 2019). Penanda gen COI juga telah diaplikasikan pada berbagai spesies ikan dari berbagai wilayah di dunia, seperti: Italia (Negri et al, 2013;Wang et al, 2012), India (Rajasekar et al, 2012), dan juga di Indonesia (Nugroho et al, 2017).…”