Genetic diversity analysis of selected Onion (Allium cepa L.) germplasm using specific RAPD and ISSR polymorphism markers

Sudha, G.; Ramesh, Palakurthi; Akila, Chandra Sekhar; Krishna, T. Sai; Bramhachari, Pallaval Veera; Riazunnisa, Khateef

doi:10.1016/j.bcab.2018.11.007

Cited by 33 publications

(21 citation statements)

References 28 publications

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“…Similar studies were conducted by Singh et al (2013) for different morphological, biochemical and bulb traits in onion who found similar results. The results of presented studies are also in accordance with the studies of Sudha et al (2019). Arya et al (2017) also studied different morphological and bulb traits for diversity analysis through principal component analysis and found that maximum traits were highly positive contributor towards total variation studied in onion.…”

Section: Discussionsupporting

confidence: 87%

Assessment of Genetic Divergence and Its Utilization in Hybrid Development in Cultivated Onion (Allium Cepa L.)

2020

THE JAPS

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Exploration of genetic variation may help to select diverse parents for open pollinated variety and hybrid development program in onion (Allium cepa L.). Thirty-five onion genotypes were analyzed by two-way cluster analysis and principal component analysis (PCA) on the basis of phenotypic traits. These genotypes were grouped in five different clusters. PCA provided information for variation in genotypes on the basis of studied traits. Ten genotypes were selected from five diverse clusters and hybridized in a line × tester mating fashion. Twenty-four F1 combinations were developed and raised along-with parents for assessment of hybrid vigor, commercial heterosis and genetic correlation. Data was analyzed for morphological and biochemical traits. Maximum commercial heterosis (heterosis computed over commercial standard/check variety) for bulb yield per plot was observed in combination MKS-SGB × MKS-GBP-01, MKS-57404 × Super Sarhad and MKS-SGB × Mustang (55.77%, 36.70% and 32.90%), respectively. Maximum better parent heterosis for bulb yield per plot was in combinations MKS-SGB × MKS-GBP-01 (31.01%), followed by MKS-TEG × Mustang, MKS-57404 × Super Sarhad and MKS-SGB × Mustang (13.76%, 12.49% and 11.79%), respectively. Genetic correlation coefficient showed that bulb yield per plot showed highly significant and positive association with single bulb weight, bulb diameter, significant positive association with leaf density (number of leaves per plant), leaf width (diameter) and significant but negative association with total soluble solids. Yield per plot showed a negative association with days to maturity. Present study revealed that cluster analysis and PCA can play an important role in effective parent selection without compromising genetic diversity for development of potential hybrids for commercial exploitation.

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Section: Discussionsupporting

confidence: 87%

Assessment of Genetic Divergence and Its Utilization in Hybrid Development in Cultivated Onion (Allium Cepa L.)

2020

THE JAPS

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“…DNA-based molecular markers, including restriction fragment length polymorphisms (RFLPs and amplified fragment length polymorphisms (AFLPs, are routinely used, however, the requirement for expensive restriction enzymes and radiolabeled probes make these techniques unsuitable for routine diagnostics [ 17 ]. Comparatively, random amplified polymorphic DNA (RAPD) markers provide a rapid, cheaper and reliable technique to identify genetic changes during in vitro culturing [ 56 ].…”

Section: Introductionmentioning

confidence: 99%

Induced in vitro adaptation for salt tolerance in date palm (Phoenix dactylifera L.) cultivar Khalas

2020

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Background: Soil salinity causes huge economic losses to agriculture productivity in arid and semiarid areas worldwide. The affected plants face disturbances in osmotic adjustment, nutrient transport, ionic toxicity and reduced photosynthesis. Conventional breeding approaches produce little success in combating various stresses in plants. However, non-conventional approaches, such as in vitro tissue culturing, produce genetic variability in the development of salt-tolerant plants, particularly in woody trees. Results: Embryogenic callus cultures of the date palm cultivar Khalas were subjected to various salt levels ranging from 0 to 300 mM in eight subcultures. The regenerants obtained from the salt-treated cultures were regenerated and evaluated using the same concentration of NaCl with which the calli were treated. All the salt-adapted (SA) regenerants showed improved growth characteristics, physiological performance, ion concentrations and K + /Na + ratios than the salt non-adapted (SNA) regenerants and the control. Regression between the leaf Na + concentration and net photosynthesis revealed an inverse nonlinear correlation in the SNA regenerants. Leaf K + contents and stomatal conductance showed a strong linear relationship in SA regenerants compared with the inverse linear correlation, and a very poor coefficient of determination in SNA regenerants. The genetic fidelity of the selected SA regenerants was also tested using 36 random amplified polymorphic DNA (RAPD) primers, of which 26 produced scorable bands. The primers generated 1-10 bands, with an average of 5.4 bands per RAPD primer; there was no variation between SA regenerants and the negative control. Conclusion: This is the first report of the variants generated from salt-stressed cultures and their potential adaptation to salinity in date palm cv. Khalas. The massive production of salt stress-adapted date palm plants may be much easier using the salt adaptation approach. Such plants can perform better during exposure to salt stress compared to the non-treated date palm plants.

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“…Polymorphism refers to the visualization of amplification based on different observed DNA fragments and score in order to determine whether the variation is presented by observing the sequence differences. RAPD is still used to analyze genetic diversity because of its ability to amplify various species, like Pinus merkusii [19] in Educational Forest Hasanuddin University, onion (Allium cepa L) [20], Dioscorea sp [21], and Indonesian Edible Canna (Canna indica L.) [22]. The comparison co-dominant and dominant primers have been performed in molecular research, in genetic diversity using RAPD and SSR of Ustilago hordei [23], and Zingiber officinale [24].…”

Section: Resultsmentioning

confidence: 99%

Selection of Dominant and Co-dominant Markers for Red Wood (Pterocarpus indicus Willd) Polymorphism from Five Provenances in East Nusa Tenggara

Larekeng¹

2019

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia

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Information about the genetic diversity of Red wood is needed to improve the quality and quantity of the plant. This information can be provided by analyzing plant DNA using molecular markers which are polymorphic and generate clear and thick bands. The objective of the study was to obtain primers that could generate clear and polymorphically bands from Red wood DNA. The evaluated Red wood was from Alor, Sika, Lembata, Rote, and Timur Tengah Selatan (TTS) provenances. As many as 54 primers screened to polymorphism assay. Those primers consisted of RAPD, SSR, and chloroplast.The SSR primers were developed from Pterocarpus indicus (six primers) and Prosopis alba (12 primers). Chloroplast primers were divided into two types, four Rubisco Large Sub-unit genes (rbcL) and 12 MaturaseK genes (matK). SSR primers of P. alba were not able to amplify Red wood DNA, whereas those of P. indicus could amplify the DNA. Three of each chloroplast primers successfully amplified the DNA, yet, each primer could only amplify one to four of 12 evaluated samples. rbcL 2 generated the highest number of sample, and samples from Lembata were amplified the most. Moreover, 16 RAPD primers were able to generate DNA.

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Genetic diversity analysis of selected Onion (Allium cepa L.) germplasm using specific RAPD and ISSR polymorphism markers

Cited by 33 publications

References 28 publications

Assessment of Genetic Divergence and Its Utilization in Hybrid Development in Cultivated Onion (Allium Cepa L.)

Assessment of Genetic Divergence and Its Utilization in Hybrid Development in Cultivated Onion (Allium Cepa L.)

Induced in vitro adaptation for salt tolerance in date palm (Phoenix dactylifera L.) cultivar Khalas

Selection of Dominant and Co-dominant Markers for Red Wood (Pterocarpus indicus Willd) Polymorphism from Five Provenances in East Nusa Tenggara

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