Genetic diversity analysis of tropical sugar beet (Beta vulgaris L.) varieties in Bangladesh using RAPD markers

Abstract

Knowledge on intra-specific genetic variation of an organism is important for its genetic improvement and conservation. In order to estimate genetic variation and relatedness in eleven tropical Sugar beet varieties we used randomly amplified polymorphic DNA (RAPD) markers. The RAPD analysis was performed using six decamer random primers, which amplified a total of 63 DNA fragments of which 43 (68.25%) were found polymorphic. The average polymorphic bands per primer was 7.17 and the overall gene diversity was 0.24. Among the 43 polymorphic loci studied, 2 were specific for 2K 310, 1 for Shubraha, 1 for Natura and 1 for HI-0473 varieties. Pair wise genetic distance and similarity indices were ranged from 0.12-0.51 and 66.73-92.91, respectively. Cauvery and 2K 310 were found to be the most distantly related with a higher genetic distance value (GD = 0.51) and lower similarity index (SI = 66.73), while Aranka and Serenada were the most closely related with their lower GD (0.12) and higher SI(92.91) values. In an unweighted pair group method of arithmetic mean dendrogram constructed on the basis of genetic distances, the eleven varieties grouped into two main clusters: 2K 310 alone was in one cluster whereas 10 other varieties grouped into a major cluster. This indicates that 2K 310 was distantly related with each of the other varieties. Distantly related varieties based on estimated genetic variation could be selected for future breeding program that could result in improvement of this crop.

In vitro regeneration and PCR-RAPD based detection of somaclonal variation in kenaf (Hibiscus cannabinus)

“…Genomic DNA extraction: Genomic DNA was isolated from leave tissues of twenty seven individuals using phonol, chloroform and isoamylalcohol extraction and ethanol precipitation method (Yasmin et al, 2006;Saclain et al, 2016). In brief, approximately 50 mg of fresh, vigorous, young actively growing leaf tissues were taken into a microcentrifuge tube, cut into small pieces, homogenized and digested with RNAse in extraction buffer (50 mM Tris-HCl, 25 mM EDTA, 300 mM NaCl, pH=8.0 and 1% SDS ).…”

“…Among them, RAPD markers are technically simple, quick to perform with small amount of DNA and do not require radioactive labeling (Michelmore et al, 1991). RAPD primers have been used for the germplasm characterization of kenaf (Cheng et al, 2000), jute (Hossain et al, 2002(Hossain et al, , 2003, roselle (Hanboonsong et al, 2000) and many other plants and animals (Bala et al, 2017;Saclain et al, 2016;Mollah et al, 2009;Rahman et al, 2007). In the present study, we report the induction of somaclonal variation caused by hormone treatments in kenaf callus and its detection using PCR-RAPD.…”

Sugar Beet Cultivation in the Tropics and Subtropics: Challenges and Opportunities

“…Sugarcane cultivation in tropical and subtropical regions is decreasing owing to high-delta crops [16]. Tropical sugar beet has been promoted in India, and its cultivation would be a good step for other tropical countries such as the United States, Brazil, China, Australia, Kenya, and South Africa [17].…”