Genetic diversity analysis using rapd marker in inbred lines of sunflower - Helianthus annuus L.

Isaacs, S.; Manivannan, N.; Muralidharan, V.

doi:10.2298/hel0339059i

Cited by 3 publications

(3 citation statements)

References 7 publications

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“…The percentage of polymorphism ranged from 80% to 100% with an average of 98.80%. The polymorphism observed here was comparatively higher than previously observed in related species like 7% in Tagetes minuta (Daroker et al 2000), 70% in Tagetes patula (Daroker et al 2000), 57.92% (Namita et al 2013) and 84% (Mor et al 2008) in marigold, 69.51% in sunflower (Isaacs et al 2003), 89.7% in Chrysanthemum morifolium (Xu et al 2006) and 83.33% in Chrysanthemum grandiflorum (Kumar et al 2014). In the present study, a wide range for the number of amplicons per primer was observed, i.e.…”

Section: Rapd Polymorphismcontrasting

confidence: 55%

Molecular characterization of African marigold (Tagetes erecta) genotypes using RAPD markers

et al. 2017

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The present investigation was carried out to characterize 22 genotypes of marigold (Tagetes erecta L.) with RAPD (randomly amplified polymorphic DNA) markers. A total of 26 RAPD markers were utilized for assessment of genetic divergence in marigold which amplified a total of 241 amplicons, out of which 238 (98.80%) were found polymorphic. The average number of amplicon per primer was 9.27. The Jaccard's similarity coefficient ranged from0.18 to 0.80. The mean values of polymorphic information content (PIC), resolving power (RP) and marker index (MI) of RAPD primers were 0.38, 8.36 and 3.44 respectively. Cluster analysis was done by unweighted pair group method for arithmetic average (UPGMA) and Principal Component analysis (PCA) was also done for the grouping of genotypes. The broad range of Jaccard's similarity coefficient observed for the genotypic data generated suggest the wide genetic base for marigold genotypes which will be effectively explored for future breeding programmes.

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Section: Rapd Polymorphismcontrasting

confidence: 55%

Molecular characterization of African marigold (Tagetes erecta) genotypes using RAPD markers

et al. 2017

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“…234B, ARM 249B, PET 2-7-1B and restores, viz. R 16, EC 623023 and RHA 138-2 falls in distinct clusters owing to their genetic dissimilarities which in turn may result in the development of heterotic hybrids (Isaacs et al 2003). The genotypes that were nearer to one another in the dendrogram were more like each other than those that were separated.…”

Section: Resultsmentioning

confidence: 96%

Study of genetic diversity using molecular markers in sunflower (Helianthus annuus)

Dhutmal

Maloo

et al. 2021

Indian J Agri Sci

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Considering the significance of sunflower (Helianthus annuus L.) cultivation under diverse agro-climatic conditions, present study was done to recognize assorted parental lines utilizing RAPD and ISSR markers to lessen environmental effects or test blunders at Rajasthan College of Agriculture, MPUA & T, Udaipur, Rajasthan during 2016. In the present study, RAPD primers produced 334 polymorphic band and ISSR primers produced 160 polymorphic bands. RAPD primers detected more polymorphic loci (56.88%) than the ISSR primers (52.24%). The dendrograms constructed by RAPD, ISSR primers and pooled data were associated with each other, indicating the efficacy of both marker systems in genetic diversity study of sunflower. Based on RAPD, ISSR and pooled data analysis, the restorer line EC 623023, R 16 and seed parental line CMS 234B, PET 2-7-1B were found to be genetically most diverse and fall into different groups. Therefore, their genetic origin seems to be different and could be efficiently used in sunflower breeding programs.

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“…Few molecular markers like simple sequence repeats (SSRs) (Iqbal et al, 2010;Garayalde et al, 2011), random amplified polymorphic DNA (RAPD) (Isaacs et al, 2003;Wangsomnuk et al, 2011a), target region amplification polymorphism (TRAP) (Yue et al, 2009;Yue et al, 2010), restriction fragment length polymorphism (RFLP) (Carrera et al, 2002), amplified fragment length polymorphism (AFLP) (Rachid et al, 2004;Dong et al, 2007;Vos et al, 1995) and inter simple sequence repeats (ISSRs) (Wangsomnuk et al, 2011b) have widely applied for marker-assisted selection (MAS), genomic evolution and used in some oilseed crops breeding research (SztubaSolińska, 2005). Mostly, genetic studies have separate principles and functions.…”

Section: Introductionmentioning

confidence: 99%

Assessment of RAPD Markers to Analyse the Genetic Diversity among Sunflower (Helianthus annuus L.) Genotypes

Raza

Shaukat

Ali

et al. 2018

Turkish JAF Sci.Tech.

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Genetic diversity estimation among different species is an important tool for genetic improvement to maximize the yield, desirable quality, wider adaptation, pest and insect resistance that ultimately boosting traditional plant breeding methods. The most efficient way of diversity estimation is application of molecular markers. In this study, twenty random amplified polymorphic DNA (RAPD) primers were utilized to estimate the genetic diversity between ten sunflower genotypes. Overall 227 bands were amplified by 20 primers with an average of 11.35 bands per primer. RAPD data showed 86.34% polymorophic bands and 13.65% of monomorophic bands. Genetic similarity was ranged from 50.22% to 87.22%. The lowest similarity (50.22%) was observed between FH-352 and FH-359 and the maximum similarity 87.22% was observed between A-23 and G-46. Polymorphic information content (PIC) values were varying from 0.05 to 0.12 with a mean of 0.09. Cluster analysis based on RAPD results displayed two major distinct groups 1 and 2. Group-2 contains FH-352 which was the most diverse genotype, while group-1 consists of few sub groups with all other genotypes. Ample diversity was found in all the genotypes. Present study reveals novel information about sunflower genome which can be used in future studies for sunflower improvement.

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Genetic diversity analysis using rapd marker in inbred lines of sunflower - Helianthus annuus L.

Cited by 3 publications

References 7 publications

Molecular characterization of African marigold (Tagetes erecta) genotypes using RAPD markers

Molecular characterization of African marigold (Tagetes erecta) genotypes using RAPD markers

Study of genetic diversity using molecular markers in sunflower (Helianthus annuus)

Assessment of RAPD Markers to Analyse the Genetic Diversity among Sunflower (Helianthus annuus L.) Genotypes

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