Genetic Diversity and Association Analysis for Salinity Tolerance, Heading Date and Plant Height of Barley Germplasm Using Simple Sequence Repeat Markers

Abstract

The objective of this study was to investigate the genetic diversity of barley accessions. Additionally, association trait analysis was conducted for grain yield under salinity, heading date and plant height. For this purpose, 48 barley genotypes were analyzed with 22 microsatellite simple sequence repeat (SSR) markers. Four of the 22 markers (Bmac316, scssr03907, HVM67 and Bmag770) were able to differentiate all barley genotypes. Cluster and principal coordinate analysis allowed a clear grouping between countries from the same region. The genotypes used in this study have been evaluated for agronomic performance in different environments. Conducting association analysis for grain yield under salinity conditions using TASSEL software revealed a close association of the marker Bmag749 (2H, bin 13) in two different environments with common significant alleles (175, 177), whereas the HVHOTR1 marker (2H, bin 3) was only significant in Sakhar_Egypt with alleles size being 158 and 161. Heading date also showed an association with scssr03907 through the common significant specific allele 111 and EBmac0415 markers in three different agro climatic locations, whereas HVCMA, scssr00103 and HVM67 were linked to heading date in the Egyptian environment only. The plant height association analysis revealed significant markers Bmag770 via the significant allele 152 and scssr09398.

Genetic Diversity Analysis of Some Barley Genotypes for Salt Tolerance Using SSR Markers

“…Also cluster of all salt tolerant diverse genotypes at seedling stage with varying marker response to cluster groups indicates that some markers are more suitable for use in marker-assisted breeding than the other and that HVHOTRI was best in marker-assisted selection followed by Bmac0316, HVM67 and Bmac 0209. These results are in a good harmony with those reported by (Eleuch et al, 2008;Chaabane et al, 2009;Aliyu et al, 2011). For the present study we can consider that these genotypes which showed salt tolerance could serve as potentially novel germplasm that could be exploited for the development of new breeding lines with high level of salinity tolerance and to accelerate genetic advancement in barley and cost-efficient than conventional screening under saline field conditions.…”

“…In addition, the same two barley cultivars no.10 (Beecher) and no. Mariey, 2004;Oraby et al, 2005;Eleuch et al, 2008). Data in Table 9 show significant interaction (LxG) between the two locations (L) and genotypes (G) for heading date.…”

“…The selection criteria of these genotypes were based on pedigrees, origin of each genotype and the genotype performance, yield and its components, heading date and plant height (Eleuch et al, 2008), based on normal distribution curve. The present investigation also intended to study molecular markers associated with salt tolerance to be useful in barley future breeding programs.…”

Genetic diversity and association analysis among Egyptian barley (Hordeum vulgare L.) genotypes with different adaptations to saline conditions analyzed by SSR markers

“…; LA and CHL, barley genotypes expressed a responsive value to seasonal changes. In the meantime, barley genotypes that showed larger LA or high CHL were not essentially those of high grain yield, Superiority in grain yield might depend on other plant characters related to root functionality (Amer et al, 2012a;Eleuch et al, 2008). The reduction in shoot length is due to excessive accumulation of salts in the cell wall elasticity.…”

Determination of Genetic Distance among Genotypes of Cucumber, Cucumis sativus L., Using ISSR Marks

“…Plant genetic sources characterized by the large genetic diversity and its ability to tolerate biogenic and non-biogenic stresses [3]. Genetic diversity contributed to provide human needs of food, clothing, medicine and energy, and even exceeded to the environmental importance, prompting plant breeders to adopt genetic diversity indicators as a source of genetic information about the crops to be used in the indirect selection of the properties associated with these indicators [1].…”