Genetic diversity and characterization of heavy metal-resistant-endophytic bacteria from two copper-tolerant plant species on copper mine wasteland

Sun, Lina; Zhang, Yanfeng; He, Linyan; Chen, Zhaojin; Wang, Qingya; Qian, Meng; Sheng, Xiafang

doi:10.1016/j.biortech.2009.08.011

Cited by 234 publications

(70 citation statements)

References 32 publications

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“…Identified isolates were submitted to NCBI with an accession number. The extraction of DNA from bacterial isolates, PCR amplification, construction of 16S rDNA clone libraries, Sequencing and phylogenetic analysis were performed as per detailed procedures given by Sun et al (2010).…”

Section: Methodsmentioning

confidence: 99%

Potential of Plant Growth Promoting Traits by Bacteria Isolated from Heavy Metal Contaminated Soils

Kumar

Singh

et al. 2015

Bull Environ Contam Toxicol

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Rhizobacteria can enhance biomass production and heavy metal tolerance of plants under the stress environment. The aim of this study was to collect soil samples from different industrial sites followed by their heavy metal analysis. After performing the ICP-AES analysis of soil samples from seven different sites, bacterial strains were isolated from the soil samples of most polluted (heavy metal) site. Phylogenetic analysis of isolates based on 16S rDNA sequences showed that the isolates belonged to four species: Bacillus thuringiensis, Azotobacter chroococcum, Paenibacillus ehimensis and Pseudomonas pseudoalcaligenes. Plant growth promoting activities; siderophore production, indole acetic acid production, HCN production, and phosphate solubilisation were assayed in vitro, and statistically analysis done by using ANOVA analysis and Tukey's Honestly Significant Difference test (p ≤ 0.05). Plant growth-promoting characteristics of isolated strains were higher compared to the control Pseudomonas fluorescens (NICM 5096). In vitro study was performed to check resistance against two heavy metals of isolates. It was observed that isolated bacterial strains have higher heavy metal resistance as compared to control E. coli (NICM 2563). These isolates may cause pathogenic effects, so to avoid this risk, their antibacterial susceptibility was checked against eight antibiotics. Among the eight antibiotics, Ciprofloxacin-1 has shown higher inhibition against all the isolated bacterial strains.

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Section: Methodsmentioning

confidence: 99%

Potential of Plant Growth Promoting Traits by Bacteria Isolated from Heavy Metal Contaminated Soils

Kumar

Singh

et al. 2015

Bull Environ Contam Toxicol

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“…Xiao et al reported a novel technology for obtaining highly efficient biosorbents from endophytes, a hyperaccumulator, which is more convenient than the traditional method of obtaining biosorbents [41]. Sun et al evaluated the genetic diversity of endophytic bacteria from the copper-tolerant species of Elshotzia apliendens and Commelina communis, reporting increased dry weights of roots and aboveground tissues compared to uninoculated plants [42]. Further, they also reported significant amounts of (ranging from 63% to 125%) Cu content in inoculated plants compared to uninoculated ones.…”

Section: Epa Regulatorymentioning

confidence: 99%

Bioremediation of Heavy Metals from Soil and Aquatic Environment: An Overview of Principles and Criteria of Fundamental Processes

et al. 2015

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Heavy metals are natural constituents of the environment, but indiscriminate use for human purposes has altered their geochemical cycles and biochemical balance. This results in excess release of heavy metals such as cadmium, copper, lead, nickel, zinc etc. into natural resources like the soil and aquatic environments. Prolonged exposure and higher accumulation of such heavy metals can have deleterious health effects on human life and aquatic biota. The role of microorganisms and plants in biotransformation of heavy metals into nontoxic forms is well-documented, and understanding the molecular mechanism of metal accumulation has numerous biotechnological implications for bioremediation of OPEN ACCESSSustainability 2015, 7 2190 metal-contaminated sites. In view of this, the present review investigates the abilities of microorganisms and plants in terms of tolerance and degradation of heavy metals. Also, advances in bioremediation technologies and strategies to explore these immense and valuable biological resources for bioremediation are discussed. An assessment of the current status of technology deployment and suggestions for future bioremediation research has also been included. Finally, there is a discussion of the genetic and molecular basis of metal tolerance in microbes, with special reference to the genomics of heavy metal accumulator plants and the identification of functional genes involved in tolerance and detoxification.

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“…Other authors (Hugenholtz 2002;Vivas et al 2008;Sun et al 2010), studying the non-culivable bacterial fraction in soils contaminated by heavy metals and other contaminants, delivered comparable results, i.e. only a minor part of analyzed 16S rDNA clone sequences revealed a higher than 89% match in terms of homology, showing the degree of sequence similarity to other known organisms.…”

Section: Identification Of Bacterial Clonesmentioning

confidence: 69%

Structure analysis of bacterial community and their heavy-metal resistance determinants in the heavy-metal-contaminated soil sample

et al. 2012

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In this study we performed the phylogenetic analysis of non-cultivable bacteria from anthropogenically disturbed soil using partial sequences of the 16S rRNA (16S rDNA) and the heavy-metal resistance genes. This soil sample contained high concentrations of nickel (2,109 mg/kg), cobalt (355 mg/kg) and zinc (177 mg/kg), smaller concentrations of iron (35.75 mg/kg) and copper (32.2 mg/kg), and also a trace amount of cadmium (<0.25 mg/kg). The 16S rDNA sequences from a total of 74 bacterial clones were distributed into four broad taxonomic groups, Acidobacteria, Actinobacteria, Bacteroidetes and Gemmatimonadetes, and some of them were unidentified. Comparing our clone sequences with those from the GenBank database, only 9 clones displayed high similarity to known bacteria belongig to actinomycetes; others were identified as uncultured ones. Among clones evidently Actinobacteria predominated. Sixteen clones from soil sample carried only the nccA-like heavy-metal-resistance genes and all sequences showed too low similarity to known proteins encoded by these genes. However, our results suggested that the heavy-metal-contaminated soil is able to present very important reservoir of the new and until now unknown partly bacteria, partly heavy-metal-resistance determinants and their products. Bacteria and nccA-like genes identified in this study could represent the objects of interest as bioremediation agents because they can be potentially used in different transformation and immobilization processes.

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Genetic diversity and characterization of heavy metal-resistant-endophytic bacteria from two copper-tolerant plant species on copper mine wasteland

Cited by 234 publications

References 32 publications

Potential of Plant Growth Promoting Traits by Bacteria Isolated from Heavy Metal Contaminated Soils

Potential of Plant Growth Promoting Traits by Bacteria Isolated from Heavy Metal Contaminated Soils

Bioremediation of Heavy Metals from Soil and Aquatic Environment: An Overview of Principles and Criteria of Fundamental Processes

Structure analysis of bacterial community and their heavy-metal resistance determinants in the heavy-metal-contaminated soil sample

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