Genetic diversity and clonal variation within the main Sicilian olive cultivars based on morphological traits and microsatellite markers

Caruso, Tiziano; Marra, Francesco Paolo; Costa, F.; Campisi, Giuseppe; Macaluso, Laura; Marchese, Annalisa

doi:10.1016/j.scienta.2014.10.019

Cited by 39 publications

(36 citation statements)

References 27 publications

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“…Furthermore, the large number of cultivars, the high degree of kinship among many of them, mainly in cases of geographic proximity, and the possible appearance of clonal variation, have raised additional identification problems (Belaj et al, 2007; Caruso et al, 2014; Ipek et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

The First Molecular Identification of an Olive Collection Applying Standard Simple Sequence Repeats and Novel Expressed Sequence Tag Markers

et al. 2017

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Germplasm collections of tree crop species represent fundamental tools for conservation of diversity and key steps for its characterization and evaluation. For the olive tree, several collections were created all over the world, but only few of them have been fully characterized and molecularly identified. The olive collection of Perugia University (UNIPG), established in the years’ 60, represents one of the first attempts to gather and safeguard olive diversity, keeping together cultivars from different countries. In the present study, a set of 370 olive trees previously uncharacterized was screened with 10 standard simple sequence repeats (SSRs) and nine new EST-SSR markers, to correctly and thoroughly identify all genotypes, verify their representativeness of the entire cultivated olive variation, and validate the effectiveness of new markers in comparison to standard genotyping tools. The SSR analysis revealed the presence of 59 genotypes, corresponding to 72 well known cultivars, 13 of them resulting exclusively present in this collection. The new EST-SSRs have shown values of diversity parameters quite similar to those of best standard SSRs. When compared to hundreds of Mediterranean cultivars, the UNIPG olive accessions were splitted into the three main populations (East, Center and West Mediterranean), confirming that the collection has a good representativeness of the entire olive variability. Furthermore, Bayesian analysis, performed on the 59 genotypes of the collection by the use of both sets of markers, have demonstrated their splitting into four clusters, with a well balanced membership obtained by EST respect to standard SSRs. The new OLEST (Olea expressed sequence tags) SSR markers resulted as effective as the best standard markers. The information obtained from this study represents a high valuable tool for ex situ conservation and management of olive genetic resources, useful to build a common database from worldwide olive cultivar collections, also based on recently developed markers.

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Section: Introductionmentioning

confidence: 99%

The First Molecular Identification of an Olive Collection Applying Standard Simple Sequence Repeats and Novel Expressed Sequence Tag Markers

et al. 2017

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“…Cytological studies have shown that the species is diploid and has a chromosome complement of (2n = 2× =46) (Breviglieri and Battaglia 1954;Minelli et al, 2000). Numerous molecular marker classes have been studied in olive, but to date they have mainly been applied to diversity studies and the characterisation of core collections (e. g. Fabbri et al, 1995, Cipriani et al, 2002, Angiolillo et al, 1999, Belaj et al, 2003, Belaj et al, 2012, Marra et al, 2013, Caruso et al, 2014, and only a few studies have employed markers to develop linkage maps for the species. The first genetic linkage maps of olive were constructed primarily from arbitrarily primed AFLP and RAPD markers, enriched with a smaller number of sequence-characterized markers such as RFLPs and microsatellites (SSRs) (De la Rosa et al, 2003;Wu et al, 2004;Khadari et al, 2010;El Aabidine et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

The first high-density sequence characterized SNP-based linkage map of olive (Olea europaea L. subsp. europaea) developed using genotyping by sequencing

et al. 2016

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A number of linkage maps have been previously developed in olive; however, these are mostly composed of markers that have not been characterized at the sequence level, supplemented with smaller numbers of microsatellite markers. In this investigation, we sought to develop a saturated linkage mapping resource for olive composed entirely of sequence characterized markers. We employed genotyping by sequencing to develop a map of a F 2 population derived from the selfing of the cultivar Koroneiki. The linkage map contained a total of 23 linkage groups comprised of 1,597 tagged SNP markers in 636 mapping bins spanning a genetic distance of 1189.7 cM. An additional 6,658 segregating SNPs were associated with the 23 linkage groups identified but their marker order was not determined in this investigation. The SNP markers sequences were submitted to NCBI database. The linkage map produced will be an invaluable resource for the study of tree habit and vigour traits segregating in the progeny, and will assist to anchor and orientate sequencing scaffolds from future genome sequencing efforts.

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“…A high number of studies dedicated at cultivar genotyping have been carried out recently, with the specific aim to solve these problems. When SSRs have been used for such purpose, they evidenced that some cultivar names refer to a polyclonal population (Bracci et al 2009;Ben-Ari et al 2014;Caruso et al 2014;Ipek et al 2012).…”

Section: Plant Cell Repmentioning

confidence: 99%

Recent developments in olive (Olea europaea L.) genetics and genomics: applications in taxonomy, varietal identification, traceability and breeding

Sebastiani

Busconi

2017

Plant Cell Rep

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The latest results in DNA markers application and genomic studies in olive. Olive (Olea europaea L.) is among the most ancient tree crops worldwide and the source of oil beneficial for human health. Despite this, few data on olive genetics are available in comparison with other cultivated plant species. Molecular information is mainly linked to molecular markers and their application to the study of DNA variation in the Olea europaea complex. In terms of genomic research, efforts have been made in sequencing, heralding the era of olive genomic. The present paper represents an update of a previous review work published in this journal in 2011. The review is again mainly focused on DNA markers, whose application still constitutes a relevant percentage of the most recently published researches. Since the olive genomic era has recently started, the latest results in this field are also being discussed.

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Genetic diversity and clonal variation within the main Sicilian olive cultivars based on morphological traits and microsatellite markers

Cited by 39 publications

References 27 publications

The First Molecular Identification of an Olive Collection Applying Standard Simple Sequence Repeats and Novel Expressed Sequence Tag Markers

The First Molecular Identification of an Olive Collection Applying Standard Simple Sequence Repeats and Novel Expressed Sequence Tag Markers

The first high-density sequence characterized SNP-based linkage map of olive (Olea europaea L. subsp. europaea) developed using genotyping by sequencing

Recent developments in olive (Olea europaea L.) genetics and genomics: applications in taxonomy, varietal identification, traceability and breeding

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