Genetic Diversity and Core Germplasm Research of 144 Munake Grape Resources Using 22 Pairs of SSR Markers

Abstract

The Munake grape is a local variety of grape that is widely distributed in Xinjiang, China. This study aims to clarify the genetic structure of the Munake grape population, characterize genetic differentiation and gene flow among populations, gather germplasm, and establish the core germplasm collection. In total, 144 samples were collected from eight geographic populations. Twenty-two SSR markers were used to characterize the genetic diversity as well as the genetic structure of Munake grape germplasm and to establish the core germplasm collection. At each site, the average number of effective alleles (Ne) was 5.019. Overall, genetic diversity was high in the various geographic populations of Munake grapes. Polymorphic information content (PIC) ranged from 0.501 to 0.908, with an average of 0.728. Estimates of genetic differentiation and gene flow indicated that the Artux population had significant genetic differences from the other populations. Screening results indicated that a sampling proportion of 95% of the sample was required to achieve 100% allelic coverage, or a sampling proportion of 65% for 95% allelic coverage. This analysis was based on conventional genetic diversity indicators, with a core germplasm diversity index of 95% coverage. Characterization of the genetic diversity of germplasm from 144 Munake grapes not only provides valuable resources for future genetic mapping and functional genome research, but also facilitates the utilization of core germplasm and molecular breeding of Munake grapes.

Genetic Diversity and Population Structure Analysis of Excellent Sugar Beet (Beta vulgaris L.) Germplasm Resources

“…Its application is intuitive and facilitates the quick understanding of crop genetic variation by analyzing phenotypic traits. However, certain drawbacks are associated with phenotypic identification, such as instability and vulnerability to environmental factors [17,18]. Molecular markers are extensively employed in population genetics due to their utility in evaluating genetic variation.…”

Phenotypic Diversity and Relationships of Fruit Traits in Persimmon (Diospyros kaki Thunb.) Germplasm Resources

“…The genetic improvement in key traits is a conventional and effective breeding approach for crops and economic trees [20], and the establishment and development of morphological traits is an important basis for genetic diversity studies. The establishment of genetic diversity in germplasm resources not only provides valuable resources for future genetic mapping and functional genome research but also facilitates the utilization of the core germplasm and molecular breeding [20,31]. Fruit phenotypic traits widely fluctuate among different persimmon germplasm; therefore, a systematic evaluation is necessary before breeding and processing.…”

SSR-marker Assisted Evaluation of Genetic Diversity in Greengram [Vigna radiata (L.) Wilcezk]

“…The genetic diversity within the genotypes of mungbean based on the morphological trait is comparatively low because it is predominantly self-pollinating (Sudha, 2009). Recently DNA markers such as SSRs have been preferred in this crop for the analysis of genetic diversity because SSR markers are locus-specific, widely dispersed throughout the genome, highly polymorphic due to variation in repeat units, highly 1 SSR-marker Assisted Evaluation of Genetic Diversity in Greengram [Vigna radiata (L.) Wilcezk] informative because of co-dominant nature, high reproducibility and ease of assay by PCR (Powell et al, 1996;Kuleung et al, 2004;Liu et al, 2013;Zia et al, 2014;Chen et al, 2015). The present study was thus undertaken to study the genetic diversity among mutants of greengram at the molecular level using SSR markers.…”