Genetic diversity and effect of natural selection at apical membrane antigen-1 (AMA-1) among Iranian Plasmodium vivax isolates

Rastaghi, Ahmad Reza Esmaeili; Nedaei, Fatemeh; Nahrevanian, Hossein; Hoseinkhan, Nazanin

doi:10.14411/fp.2014.048

Cited by 4 publications

(3 citation statements)

References 30 publications

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“…For example, comprehensive research is needed to develop vaccine strategy. Though antigenic variation is one of the major challenges that affects the development of malaria vaccine ( Esmaeili Rastaghi et al., 2014 ), advanced knowledge of parasite antigenic variants is a prerequisite for the rational design of a vaccine that might be efficient in various endemic areas.…”

Section: Discussionmentioning

confidence: 99%

“…Meanwhile, due to the highly polymorphic feature of the PvAMA-1 gene, it has been used as molecular marker for population genetic studies (Joshi, 2003). However, antigenic variation is a major challenge in the design of protective malaria vaccine (Esmaeili Rastaghi et al, 2014). Recently, a report on genetic diversity of Pvama-1 in India also showed this trend (Kale et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity and Selection of Plasmodium vivax Apical Membrane Antigen-1 in China–Myanmar Border of Yunnan Province, China, 2009–2016

Cui

Shen

Chen

et al. 2022

Front. Cell. Infect. Microbiol.

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Plasmodium vivax apical membrane antigen-1 (PvAMA-1) is an important vaccine candidate for vivax malaria. However, antigenic variation within PvAMA-1 is a major obstacle to the design of a global protective malaria vaccine. In this study, we analyzed the genetic polymorphism and selection of the PvAMA-1 gene from 152 P. vivax isolates from imported cases to China, collected in the China–Myanmar border (CMB) area in Yunnan Province (YP) during 2009–2011 (n = 71) and 2014–2016 (n = 81), in comparison with PvAMA-1 gene information from Myanmar (n = 73), collected from public data. The overall nucleotide diversity of the PvAMA-1 gene from the 152 YP isolates was 0.007 with 76 haplotypes identified (Hd = 0.958). Results from the population structure suggested three groups among the YP and Myanmar isolates with optimized clusters value of K = 7. In addition, YP (2014–2016) isolates generally lacked some K components that were commonly found in YP (2009–2011) and Myanmar. Meanwhile, PvAMA-1 domain I is found to be the dominant target of positive diversifying selection and most mutation loci were found in this domain. The mutation frequencies of D107N/A, R112K/T, K120R, E145A, E277K, and R438H in PvAMA-1 were more than 70% in the YP isolates. In conclusion, high genetic diversity and positive selection were found in the PvAMA-1 gene from YP isolates, which are significant findings for the design and development of PvAMA-1-based malaria vaccine.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Selection of Plasmodium vivax Apical Membrane Antigen-1 in China–Myanmar Border of Yunnan Province, China, 2009–2016

Cui

Shen

Chen

et al. 2022

Front. Cell. Infect. Microbiol.

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“…For SLK, n = 23: EF218679-EF218701 [ 13 ]. For Iran (IRN), n = 83: JX624732-35 [ 52 ], KF422636-681, and KF422636-KF422681 [ 53 ]. For Papua New Guinea (PNG), n = 102: KC702402-KC702503 [ 54 ].…”

Section: Methodsmentioning

confidence: 99%

Diversity and evolutionary genetics of the three major Plasmodium vivax merozoite genes participating in reticulocyte invasion in southern Mexico

et al. 2015

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BackgroundReported malaria cases in the Americas had been reduced to about one-half million by 2012. To advance towards elimination of this disease, it is necessary to gain insights into how the malaria parasite is evolving, including the emergence, spread and persistence of new haplotypes in affected regions. In here, the genetic diversity of the three major P. vivax merozoite genes was analyzed.MethodsFrom P. vivax-infected blood samples obtained in southern Mexico (SMX) during 2006–2007, nucleotide sequences were achieved for: the 42 kDa carboxyl fragment of the merozoite surface protein-1 (msp142), domains I-II of the apical membrane antigen-1 (ama1I-II), and domain II of the Duffy binding protein (dbpII). Gene polymorphism was examined and haplotype networks were developed to depict parasite relationships in SMX. Then genetic diversity, recombination and natural selection were analyzed and the degree of differentiation was determined as FST values.ResultsThe diversity of P. vivax merozoite genes in SMX was less than that of parasites from other geographic origins, with dbpII < ama1I-II < msp142. Ama1I-II and msp142 exposed the more numerous haplotypes exclusive to SMX. While, all dbpII haplotypes from SMX were separated from one to three mutational steps, the networks of ama1I-II and msp142 were more complex; loops and numerous mutational steps were evidenced, likely due to recombination. Sings of local diversification were more evident for msp142. Sixteen combined haplotypes were determined; one of these haplotypes not detected in 2006 was highly frequent in 2007. The Rm value was higher for msp142than for ama1I-II, being insignificant for dbpII. The dN-dS value was highly significant for ama1I-II and lesser so for dbpII. The FST values were higher for dbpII than msp142, and very low for ama1I-II.ConclusionsIn SMX, P. vivax ama1I-II, dbpII and msp142 demonstrated limited diversity, and exhibited a differentiated parasite population. The results suggest that differential intensities of selective forces are operating on these gene fragments, and probably related to their timing, length of exposure and function during reticulocyte adhesion and invasion. Therefore, these finding are essential for mono and multivalent vaccine development and for epidemiological surveillance.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-015-1266-7) contains supplementary material, which is available to authorized users.

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Low levels of genetic diversity associated with evidence of negative selection on the Babesia bovis apical membrane antigen 1 from parasite populations in Thailand

Rittipornlertrak

Nambooppha

Simking

et al. 2017

Infection, Genetics and Evolution

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Genetic diversity and effect of natural selection at apical membrane antigen-1 (AMA-1) among Iranian Plasmodium vivax isolates

Cited by 4 publications

References 30 publications

Genetic Diversity and Selection of Plasmodium vivax Apical Membrane Antigen-1 in China–Myanmar Border of Yunnan Province, China, 2009–2016

Genetic Diversity and Selection of Plasmodium vivax Apical Membrane Antigen-1 in China–Myanmar Border of Yunnan Province, China, 2009–2016

Diversity and evolutionary genetics of the three major Plasmodium vivax merozoite genes participating in reticulocyte invasion in southern Mexico

Low levels of genetic diversity associated with evidence of negative selection on the Babesia bovis apical membrane antigen 1 from parasite populations in Thailand

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