Genetic Diversity and Geographic Differentiation in Endangered Ammopiptanthus (Leguminosae) Populations in Desert Regions of Northwest China as Revealed by ISSR Analysis

Ge, Xue Jun

doi:10.1093/aob/mci089

Cited by 105 publications

(87 citation statements)

References 22 publications

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“…It can survive under annual precipitation conditions of less than 50 mm and annual evaporation over 3000 mm (Liu, 1998). In addition, it helps to prevent sand movement, thus delaying further desertification (Ge et al, 2005). Due to its ecological importance and high academic value, A. mongolicus has become a good model for studying plants under abiotic stress conditions.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of potential simple sequence repeat markers in Ammopiptanthus mongolicus

Jin

Han

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Ammopiptanthus mongolicus, an evergreen broadleaf legume shrub, can survive under conditions of high and low temperature, extreme salinity, and drought. This attribute makes it an ideal model for studying mechanisms of stress tolerance in plants. However, simple sequence repeat (SSR) resources for this species are insufficient in public databases. In this study, a total of 44,959 unigenes identified from the A. mongolicus transcriptome were used for SSR analysis by MIcroSAtellite (MISA). A total of 13,859 SSRs were found to be distributed within 10,409 unigenes, with an average length of 15 bp and an average density of one SSR per 4.4 kb. There were 222 different motif types in the A. mongolicus transcriptome, and mononucleotide repeats represented the main type, accounting for 44.2% of all SSRs. The (A/T)n repeat was the most frequent motif, accounting for 42.37% of all SSRs. We also performed Gene Ontology functional analysis, Kyoto Encyclopedia of Genes and Genomes database pathway analysis, and eggNOG analysis, and identified 6157, 2301, and 9845 unigenes containing SSRs in these three databases, respectively. The functional categorization of A. mongolicus unigenes containing SSRs revealed that these unigenes represent many transcribed genes with different functions. These data provide sequence information that may be used to improve molecular-assisted markers for the study A. mongolicus genetic diversity.

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Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of potential simple sequence repeat markers in Ammopiptanthus mongolicus

Jin

Han

et al. 2016

Genet. Mol. Res.

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“…This region was represented by only three populations (PN25, 26,28). The central region was represented by eleven populations (PN2, 4,13,14,15,18,20,22,23,24,29). In the southern (especially south-eastern) region of Lithuania, there are many locations that are occupied by this invasive species or where it is otherwise very frequent.…”

Section: Methodsmentioning

confidence: 99%

“…Reduced genetic variability is typically found in such populations of invasive species, due to marked founder effects and reproductive systems limiting the diversity of genotype [7]. The lower genetic diversity of some invasive plant populations has been shown with molecular markers such as ITS [8], RAPD [9][10][11] and ISSR [11][12][13][14]. In contrast, rather high genetic diversity has been shown in populations of the apomictic invaders Taraxacum, as revealed with AFLP markers [15], and Erigeron annuus, as revealed using RAPD and AFLP [16,17] markers.…”

Section: Introductionmentioning

confidence: 99%

Clonal structure and reduced diversity of the invasive alien plant Erigeron annuus in Lithuania

et al. 2013

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The alien species Erigeron annuus (L.) Pers. is in an intensive spreading phase in Lithuania. Random amplified polymorphic DNA (RAPDs) and inter-simple sequence repeats (ISSRs) assays were used to study the genetic structure of old and new invasive populations and to determine the most spread genotypes of this species in Lithuania. Pairwise genetic distances between populations established using RAPD and ISSR markers significantly correlated (r=0.91, P<0.05). Our study indicates that there are two genetically different types of E. annuus populations. The first type is represented by a widely spread main clone and related monomorphic populations. The second type is represented by polymorphic populations, some of them present at sites where E. annuus has not been previously observed. Main clone predominates in nine populations and is from the region where this species was first described in natural ecosystems of Lithuania. UPGMA cluster analysis revealed genetic relationships between the main clone and accessions from old cemeteries where E. annuus has been grown as an ornamental plant. We found high genetic differentiation among populations (G ST=0.58 for RAPDs, G ST=0.64 for ISSRs). Taken together, our results will contribute to the monitoring of E. annuus spread in Lithuania.

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“…In the present study, we used inter-simple sequence repeat (ISSR) analysis to examine the genetic differentiation within P. odoratissimus populations. This sensitive, reproducible, and cost-efficient method can be used to i n v e s t i g a t e t h e g e n e t i c s t r u c t u r e a n d g e n e t i c differentiation within and among populations of limited or widespread species (Alexander et al 2004, Deshpande et al 2001, Ge et al 2005, Hu et al 2008, McCauley and Ballard 2002, Rossi et al 2009, Xiao et al 2004. ISSR was also reported as a useful method for analyzing species that lacked available sequence information, because this m e t h o d d o e s n o t r e q u i r e p r i o r D N A s e q u e n c e information before amplification (Hu et al 2008).…”

Section: Sexual Reproduction In P Odoratissimus Is Achievedmentioning

confidence: 99%

Differences in the growth of beach Pandan, Pandanus odoratissimus, between tropical (Java) and subtropical (southern Japan) zones

Susanti

Suzuki

Miyamoto

et al. 2012

Tropics

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The coastal plant Pandanus odoratissimus L.f. is distributed from the tropics to the subtropics. We studied this species to clarify the differences in its population dynamics and growth strategies between populations at a tropical plot at Cipatujah, West Java, Indonesia (7˚46 S, 108˚04 E) and at subtropical plot at Tokuno-shima Island, Japan (27˚53 N, 128˚57 E). We measured plant morphological characteristics and estimated plant growth from stem elongation during a one year period. We analysed genetic differentiation using five inter-simple sequence repeat (

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Genetic Diversity and Geographic Differentiation in Endangered Ammopiptanthus (Leguminosae) Populations in Desert Regions of Northwest China as Revealed by ISSR Analysis

Abstract: The significant genetic difference between the two species might be due to a possible vicariant evolutionary event from a single common ancestor through the fragmentation of their common ancestor's range. Conservation strategies for these two endangered species are proposed.

Cited by 105 publications

References 22 publications

Transcriptome analysis of potential simple sequence repeat markers in Ammopiptanthus mongolicus

Transcriptome analysis of potential simple sequence repeat markers in Ammopiptanthus mongolicus

Clonal structure and reduced diversity of the invasive alien plant Erigeron annuus in Lithuania

Differences in the growth of beach Pandan, Pandanus odoratissimus, between tropical (Java) and subtropical (southern Japan) zones

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