Genetic diversity and geographic distribution of Bemisia tabaci species complex in Nepal

Acharya, Rajendra; Shrestha, Yam Kumar; Sharma, Sushant Raj; Lee, Kyeong-Yeoll

doi:10.1016/j.aspen.2020.03.014

Cited by 7 publications

(12 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SLCCNV was distributed in the whole country while the other six viruses were distributed in the middle and west regions of Nepal. In comparison with our previous study [31], the begomovirus profile is highly associated with the geographic distribution of three cryptic species of B. tabaci. Particularly, investigation at the eastern region of Nepal showed that distribution of only Asia II 5 which ingested mostly SLCCNV.…”

Section: Discussioncontrasting

confidence: 54%

“…Our finding is the first report of SLCCNV presence in Nepal. This virus was detected in the Asia I, Asia II 1, and mostly from Asia II 5 species, which is the most abundant cryptic species of B. tabaci in Nepal [31]. In a previous study, Khatun et al [40] detected SLCCNV only in the Asia I species.…”

Section: Discussionmentioning

confidence: 88%

“…In our previous study on the genetic and geographic distribution of B. tabaci species complex in Nepal, we found three cryptic species: Asia I, Asia II 1, and Asia II 5 [31]. We expected that the geographic distribution of begomoviruses that were ingested by different cryptic species of B. tabaci can be determined by analyzing whitefly samples in Nepal.…”

Section: Introductionmentioning

confidence: 95%

“…We collected seventy-six B. tabaci samples during the summer of 2017-2019 across 23 districts from east to west Nepal (Table 1) for identification of B. tabaci cryptic species and their geographic distribution, which were reported in our previous study [31]. Samples were collected from different host plants, including vegetables (Abelmoschus esculentus, Cucurbita pepo, Cucumis sativus, Phaseolus vulgaris, and Sechium edule,), oilseed crop (Sesamum indicum), flowers (Bougainvillea spectabilis, Hibiscus rosa-sinensis, Ixora sp., and Salvia officinalis), tuber crop (Smallanthus sonchifolius) and fruit plant (Psidium guajava).…”

Section: Whitefly Samplesmentioning

confidence: 99%

See 3 more Smart Citations

Identification of Begomoviruses from Three Cryptic Species of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in Nepal

et al. 2021

Self Cite

View full text Add to dashboard Cite

The Bemisia tabaci species complex consists of at least 44 cryptic species, which are potential vectors of approximately 320 begomovirus species, most of which are significant plant viruses. However, the relationship of begomovirus transmission through vectors at the cryptic species level is uncertain. In our previous study, three cryptic species (Asia I, Asia II 1, and Asia II 5) of B. tabaci were identified from 76 B. tabaci samples collected across 23 districts in Nepal. Using the same individuals we identified seven different begomovirus species (Squash leaf curl China virus [SLCCNV], Tomato leaf curl New Delhi virus [ToLCNDV], Okra enation leaf curl virus [OELCuV], Synedrella leaf curl virus [SyLCV], Tomato leaf curl Kerala virus [ToLCKeV], Ageratum enation virus [AEV], and Tomato leaf curl Karnataka virus [ToLCKV]) by PCR using universal begomovirus primers. The begomoviruses were detected in 55.26% of whitefly samples, and SLCCNV was the most prevalent species (27.63%). Among the three cryptic species of B. tabaci, the virus detection rate was highest in Asia I (60%), followed by Asia II 1 (58.82%) and Asia II 5 (53.06%). Most viruses were detected in all three species, but AEV and ToLCKV were found only in Asia I and Asia II 1, respectively. Geographic analysis showed that SLCCNV was distributed in the whole country, which is similar to the distribution of the Asia II 5 species, but OELCuV and SyLCV were detected only in the middle region of Nepal. Our results provide important information on the begomovirus profile in Nepal which can be beneficial for plant virus risk assessment and develop the management strategies to reduce the damage of whitefly transmitted viruses.

show abstract

Section: Discussioncontrasting

confidence: 54%

Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 95%

Section: Whitefly Samplesmentioning

confidence: 99%

See 2 more Smart Citations

Identification of Begomoviruses from Three Cryptic Species of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in Nepal

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Among them, the genetic groups of only Asia II 5 and Asia II 7 were detected in the collected whitefly samples spanning across the Bengal province. Previous reports suggest that Asia II 5 was majorly prevalent in the South Asian countries like Bangladesh, Pakistan, and Nepal (Islam et al, 2018 ; Khatun et al, 2018 ; Acharya et al, 2020 ). In the survey conducted, Asia II 5 was found to be majorly distributed along the southern part of Bengal and associated with a single host crop, that is, eggplant.…”

Section: Discussionmentioning

confidence: 96%

Unraveling the Basis of Neonicotinoid Resistance in Whitefly Species Complex: Role of Endosymbiotic Bacteria and Insecticide Resistance Genes

et al. 2022

View full text Add to dashboard Cite

Bemisia tabaci (whitefly) is one of the most detrimental agricultural insect pests and vectors of many plant viruses distributed worldwide. Knowledge of the distribution patterns and insecticide resistance of this cryptic species is crucial for its management. In this study, genetic variation of mitochondrial cytochrome oxidase subunit 1 (MtCoI) gene of B. tabaci was analyzed followed by a study of the infection profile of various endosymbionts in 26 whitefly populations collected from West Bengal, India. Phylogenetic analysis revealed Asia I as the major cryptic species (65.38%), followed by Asia II 5, China 3, and Asia II 7, which were diversified into 20 different haplotypes. In addition to the primary endosymbiont (C. poriera), each of the four whitefly species showed a variable population of three secondary endosymbionts, majorly Arsenophonus with the highest infection rate (73.07%), followed by Wolbachia and Rickettsia. Further phylogenetic analyses revealed the presence of two subgroups of Arsenophonus, viz., A1 and A2, and one each in Wolbachia (W1) and Rickettsia (R3). Resistance to thiamethoxam, imidacloprid, and acetamiprid insecticides was analyzed for a clear picture of pesticide resistance status. The highest susceptibility was noted toward thiamethoxam (LC50 = 5.36 mg/L), followed by imidacloprid and acetamiprid. The whitefly population from Purulia and Hooghly districts bearing Asia II 7 and Asia II 5 cryptic species, respectively, shows maximum resistance. The differences in mean relative titer of four symbiotic bacteria among field populations varied considerably; however, a significant positive linear correlation was observed between the resistance level and relative titer of Arsenophonus and Wolbachia in the case of imidacloprid and thiamethoxam, while only Wolbachia was found in case of acetamiprid. Expression analysis demonstrated differential upregulation of insecticide resistance genes with Purulia and Hooghly populations showing maximally upregulated P450 genes. Moreover, thiamethoxam and imidacloprid resistance ratio (RR) showed a significant correlation with CYP6CM1, CYP6DZ7, and CYP4C64 genes, while acetamiprid RR correlated with CYP6CX1, CYP6DW2, CYP6DZ7, and CYP4C64 genes. Taken together, these findings suggested that P450 mono-oxygenase and symbiotic bacteria together affected whitefly resistance to neonicotinoids. Hence, a symbiont-oriented management programme could be a better alternative to control or delay resistance development in whitefly and can be used for pesticide clean-up in an agricultural field.

show abstract

Genetic diversity of cryptic species of Bemisia tabaci in Asia

Lestari

Khatun

Acharya

et al. 2022

Arch Insect Biochem Physiol

Self Cite

View full text Add to dashboard Cite

show abstract

Genetic diversity and geographic distribution of Bemisia tabaci species complex in Nepal

Cited by 7 publications

References 41 publications

Identification of Begomoviruses from Three Cryptic Species of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in Nepal

Identification of Begomoviruses from Three Cryptic Species of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in Nepal

Unraveling the Basis of Neonicotinoid Resistance in Whitefly Species Complex: Role of Endosymbiotic Bacteria and Insecticide Resistance Genes

Genetic diversity of cryptic species of Bemisia tabaci in Asia

Contact Info

Product

Resources

About