Genetic diversity and identification of some Turkish cotton genotypes (Gossypium hirsutum L.) by RAPD-PCR analysis

Surgun, Yonca; Çöl, Bekir; Bürün, Betül

doi:10.3906/biy-1101-167

Cited by 14 publications

(3 citation statements)

References 16 publications

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“…Amplification was carried out in a SensoQuest thermocycler with a cycle programmed for 45 cycles of each of the following conditions: initial denaturation at a temperature of 94C for 2 minutes, followed by denaturation at 94C for 1 minute, annealing at 34C for 1 minute, elongation at 72C for 2 minutes 20 seconds, and the final condition was elongation at 72C for 10 minutes. OPA-02 TGCCGAGCTG [24] OPA-03 AGTCAGCCAC [25] OPA-07 GAAACGGGTG [25] OPA-08 GTGACGTAGG [26] OPA-10 GTGATCGCAG [25] OPA-13 CAGCACCCAC [25] OPA-16 AGCCAGCGAA [27] OPB-08 GTCCACACGG [28] OPB-10 CTGCTGGGAC [29] Electrophoresis…”

Section: Rapd Primer Selection and Dna Amplificationmentioning

confidence: 99%

Genetic Variation of Ceiba pentandra (L.) Gaertn. from Three Populations in West Sumatra, Indonesia Based on RAPD Markers

Hefzi,

Mansyurdin,

Maideliza

et al. 2023

IJPSAT

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Ceiba pentandra (L.) Gaertn. is one of the cotton-producing plants. However, there is a population decline in some areas, resulting in a decrease in genetic variation. This study aims to determine intrapopulation and interpopulation genetic variation and genetic differentiation of C. pentandra in three populations in West Sumatra. The research was conducted with the descriptive method using molecular data and sample collection with the survey method. Analysis of genetic variation using RAPD (Random Amplified Polymorphic DNA) markers on 15 individual plants collected from three populations, namely Solok, Pesisir Selatan, and Padang Pariaman. The results showed that primers OPA-01, OPA-02, and OPB-10 could detect polymorphism. The value of intrapopulation genetic variation was highest in the South Coastal population (H = 0.1857) and lowest in the Solok population (H = 0.1228). Interpopulation genetic variation (DST = 0.0326) was lower than intrapopulation genetic variation (Hs = 0.1607), with a low genetic differentiation value (GST = 0.1686) and a high gene flow value (Nm = 2.4660). Cluster analysis showed that the Pesisir Selatan and Solok populations had the farthest genetic distances (0.0580). UPGMA analysis shows that accessions do not cluster specifically based on their population.

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Section: Rapd Primer Selection and Dna Amplificationmentioning

confidence: 99%

Genetic Variation of Ceiba pentandra (L.) Gaertn. from Three Populations in West Sumatra, Indonesia Based on RAPD Markers

Hefzi,

Mansyurdin,

Maideliza

et al. 2023

IJPSAT

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“…It is revealed that BRs lead to distinct cell responses such as stem elongation, root and leaf development, leaf bending and epinasty, formation and development of the pollen tube, reproductive development, xylem differentiation, proton-pomp activation, and regulation of gene expression (Yang et al 2011;. In addition to their roles in the development of plants, BRs confer stress tolerance on plants against different abiotic stresses such as salt, heat, cold, drought, and heavy metals (Surgun et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Determination of effects 24-epibrassinolide in tomato (Solanum lycopersicum L.) shoot tip cultures under NaCl stress

Yılmaz Gökdoğan,

Bürün

2024

J Agr Sci-Tarim Bili

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The negative effects of salt stress on plants and their environment are increasing dramatically day by day, and it is very crucial for plants to develop salt tolerance with various applications. To this end, in recent years, there is a need for studies to increase salt tolerance with in vitro cultures in which controlled conditions are provided as an alternative approach to greenhouse and pot experiments, which are affected by environmental external conditions. In this study, 24-epibrassinolide (24-epiBL) was used for increasing salt tolerance via in vitro shoot tip cultures of tomato M-28 hybrid cultivar. Shoot tips of 10-day sterile seedlings were placed in MS medium supplemented with 2 mg L-1 K + 0.4 mg L-1 NAA in 12-day culture period, and 12-day old plantlets soaked in 24-epiBL solutions (0, 1, 2 µM) were transferred to MS medium containing different concentrations of NaCl (0, 20, 40, 60, 80, 100 mM). After 20 days, it was determined that morphological parameters (lenght, fresh and dry weight of plantlets) and biochemical parameters (pigment, MDA, proline, total soluble protein contents, POX and SOD enzyme activities). All developmental parameters and various biochemical parameters such as pigment and total soluble protein contents negatively affected by NaCl stress, whereas MDA, proline content, SOD and POX enzyme activities increased. It was found that increase in fresh weight, pigment content, SOD and POX enzyme activity with 24-epiBL pretreatment agaist NaCl stress were found statistically significant difference. Hovewer MDA content decreased with 24-epiBL pretreatment under NaCl. As a result, NaCl responses in plant varies between different NaCl and 24-epiBL concentrations, and the various deffence strategies combine multiple tolerance mechanisms. It was determined that 24-epiBL may play a crucial role increasing salt tolerance using in vitro shoot tip cultures of tomato M-28 hybrid cultivar.

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“…Furthermore, no prior knowledge of the sequence being studied is required. In diversity analysis, RAPD markers have proven to be effective (Surgun et al, 2012).Genetic diversity is one parameter that may help estimate which species are more likely to adapt to future situations. In general, genetic diversity refers to any measure that quantifies variation in alternate forms of genes or non-coding locations within a population (Hughes, 2008).…”

Section: Introductionmentioning

confidence: 99%

ASSESSMENT OF GENETIC DIVERSITY BY RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) AND ATPASE 6/8 GENE IN Pangasiuspangasius

2023

THE JAPS

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The aim of the present study was to use RAPD and ATPase markers to measure and analyze the level of genetic variation within farm cultured populations of introduced Pangas catfish Pangasius pangasius to evaluate their potential in ascertaining genetic differentiation. Hence, genetic diversity of farmed P.pangasius was carried out from five different sites by using RAPD primer. Primers showed 151 total amplified locus with an average of 37.75 loci. The highest number of bands were observed in P.pangasius from population 1, maximum no of bands was observed in OPA-02 (56 bands), while the minimum from OPA-05 (26 bands). Minimum polymorphism was observed in OPA-08 (10.71%), while maximum in OPA-01 (66.67%). The value of heterozygosity of P. pangasius ranged from 0.123 to 0.444.A cluster between population 1 and 2, 2 nd population 3 and 4, and population 5 with all other as 3 rd cluster. Distance found maximum in population 5 that showed heterozygosity. The genetic variations and polymorphism among populations showed the degree of intra and inter-population genetic diversity in P. pangasius populations. Study is also done to access the genetic diversity by ATPase 6/8 gene. ATPase 6/8 showed highest haplotype diversity (0.800) in the P. pangasius population of Tariq Khan Faheem Fish Farm, while the lowest (0.700) in Al-Raheem fish Farm. Highest nucleotide diversity (0.5703) in Tariq Khan Faheem Fish Farm, while lowest (0.5026) in Mirza Rizwan Ahmed Fish farm. Higher diversity is important for conservation and management of population.Random amplified polymorphic DNA (RAPD) and ATPase 6/8 markers were used to estimate the genetic diversity and population structure among the samples of farmed P. pangasius. Overall, RAPD and ATPase 6/8 proved as valuable tool for reliable and quick stock discrimination and provided information that might be useful regarding management and conservation of P. pangasius. The findings will be helpful in developing stock specific management measures for conservation and sustainable utilization of the species.

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Genetic diversity and identification of some Turkish cotton genotypes (Gossypium hirsutum L.) by RAPD-PCR analysis

Cited by 14 publications

References 16 publications

Genetic Variation of Ceiba pentandra (L.) Gaertn. from Three Populations in West Sumatra, Indonesia Based on RAPD Markers

Genetic Variation of Ceiba pentandra (L.) Gaertn. from Three Populations in West Sumatra, Indonesia Based on RAPD Markers

Determination of effects 24-epibrassinolide in tomato (Solanum lycopersicum L.) shoot tip cultures under NaCl stress

ASSESSMENT OF GENETIC DIVERSITY BY RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) AND ATPASE 6/8 GENE IN Pangasiuspangasius

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