Genetic diversity and molecular characterization of puroindoline genes ( Pina-D1 and Pinb-D1 ) in bread wheat landraces from Andalusia (Southern Spain)

Ayala, Marcela; Guzmán, Carlos; Peña, Roberto J.; Álvarez, José Ramón Guzmán

doi:10.1016/j.jcs.2016.07.017

Cited by 9 publications

(11 citation statements)

References 24 publications

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“…They were represented by a similar variant number (11-16%). Similar distribution of the same Pinb alleles was observed previously in European wheat populations [15,24,43]. As for the landraces, the Pinb allele distributions were different.…”

Section: Discussionsupporting

confidence: 85%

“…Surprisingly, no allelic variability within the Pina gene was observed, although mutant alleles of this gene were detected in many other European cultivars [15,24,42]. All tested cultivars, breeding lines, and landraces had the wild-type Pina-D1a allele.…”

Section: Discussionmentioning

confidence: 89%

“…Genetic diversity and grain hardness allelic variation were studied in Australian commercial wheat cultivars [22], the Watkins collection [23], and bread wheat landraces from Andalusia [24]. Additionally, the trait is also easily genetically engineered [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

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The Variability of Puroindoline-Encoding Alleles and Their Influence on Grain Hardness in Modern Wheat Cultivars Cultivated in Poland, Breeding Lines and Polish Old Landraces (Triticum aestivum L.)

et al. 2020

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Wheat (Triticum aestivum L.) grain hardness is determined mainly by variations in puroindoline genes (Pina-D1 and Pinb-D1), which are located on the short arm of chromosome 5D. This trait has a direct effect on the technological properties of the flour and the final product quality. The objective of the study was to analyze the mutation frequency in both Pin genes and their influence on grain hardness in 118 modern bread wheat cultivars and breeding lines cultivated in Poland, and 80 landraces from Poland. The PCR products containing the Pin gene coding sequences were sequenced by the Sanger method. Based on detected the SNPs (single-nucleotide polymorphisms) we designed CAPS (cleaved amplified polymorphic sequence) markers for the fast screening of Pinb alleles in a large number of genotypes. All analyzed cultivars, breeding lines, and landraces possess the wild-type Pina-D1a allele. Allelic variation was observed within the Pinb gene. The most frequently occurring allele in modern wheat cultivars and breeding lines (over 50%) was Pinb-D1b. The contribution of the remaining alleles (Pinb-D1a, Pinb-D1c, and Pinb-D1d) was much less (approx. 15% each). In landraces, the most frequent allele was Pinb-D1a (over 70%), followed by Pinb-D1b (21% frequency). Pinb-D1c and Pinb-D1g were found in individual varieties. SKCS (single-kernel characterization system) analysis revealed that grain hardness was strictly connected with Pinb gene allelic variation in most tested cultivars. The mean grain hardness values were significantly greater in cultivars with mutant Pinb variants as compared to those with the wild-type Pinb-D1a allele. Based on grain hardness measured by SKCS, we classified the analyzed cultivars and lines into different classes according to a previously proposed classification system.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 89%

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The Variability of Puroindoline-Encoding Alleles and Their Influence on Grain Hardness in Modern Wheat Cultivars Cultivated in Poland, Breeding Lines and Polish Old Landraces (Triticum aestivum L.)

et al. 2020

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“…Two additional rare Pinb-D1 alleles were found. Fifteen landraces carried an allele previously described only in Spanish landraces and spelt wheat, not included in the Catalogue of Gene Symbols, but tentatively classified as the allele Pinb-D1ad [ 45 ]. This allele has a C/T change in position 271.…”

Section: Resultsmentioning

confidence: 99%

Exploring the End-Use Quality Potential of a Collection of Spanish Bread Wheat Landraces

López-Fernández

Pascual

Faci

et al. 2021

Plants

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Modern plant-breeding practices have narrowed the genetic base of wheat, such that there is a need to introduce new germplasms with underexploited diversity into breeding programs. Wheat landraces are a very valuable resource when searching for genetic variation, which not only possess increased adaptability, but also quality-related traits. Several studies have shown a wide genetic diversity in Spanish wheat landraces compared to other germplasm collections; therefore, the main objective of this study is to analyze the variability in a collection of 189 landraces from the Spanish National Plant Genetic Resources Centre (Centro de Recursos Fitogenéticos, CRF-INIA, Alcalá de Henares), in relation to end-use quality traits. We characterized the whole collection for high-molecular-weight glutenin and puroindoline allelic composition, and for gluten strength. In addition, grain protein content, grains per spike, and thousand kernel weight were evaluated in samples from four-year field trials. The relationship between glutenin composition and quality was evaluated, and some alleles strongly associated with high quality were identified in the collection, some of them specific for Iberian landraces. The results also show the presence of novel variability within high-molecular-weight glutenin and puroindolines, which needs to be characterized further in order to assess its influence on wheat quality. In addition, a set of landraces showing outstanding values for gluten quality and a good agronomic performance was selected for testing in field trials in order to evaluate the suitability of their direct use in cropping systems.

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“…Finally, the investigation of wheat genetic resources to discover sources of new genes and alleles, and quality traits that are not common in the modern wheat pool is another important component of CIMMYT's research portfolio. Collections of bread and durum wheat landraces, wheat ancestors (including einkorn, emmer and spelt), and wild relatives (mainly Aegilops species) have been evaluated for traits related to wheat quality [38][39][40][41][42][43][44][45][46] . In these collections, novel genetic variability and promising genotypes with atypical phenotypic characteristics have been identified and can be considered as valuable parent materials to be exploited in breeding programs targeting improvement of quality traits and widening the range of the end-uses for wheat grain.…”

Section: Wheat Grain Quality Research Activitiesmentioning

confidence: 99%

Genetic improvement of wheat grain quality at CIMMYT

Guzmán

Ammar

Govindan

et al. 2019

Front. Agr. Sci. Eng.

Self Cite

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The International Maize and Wheat Improvement Center (CIMMYT) is the global leader in publiclyfunded maize and wheat research and in farming systems based on these crops. CIMMYT leads the Global Wheat Program (GWP), which includes some of the largest wheat breeding programs in the world. The GWP has been successful in developing wheat germplasm that is used extensively worldwide. Wheat quality improvement is a central component of all the breeding efforts at CIMMYT and the Wheat Chemistry and Quality Laboratory represents an integral part of the breeding programs. Wheat quality is addressed at CIMMYT over the full range of this very wide and variable concept with milling, processing, end-use and nutritional quality targeted. Wheat progenitors and advanced lines developed by the breeders are assessed for diverse quality attributes, with the aim of identifying those that fulfill the requirements in terms of milling, processing, end-use and nutritional quality in different target regions. Significant research is conducted to make more efficient the integration of wheat quality traits in the breeding programs. The main topics being addressed are (1) methodologies to analyze grain quality traits, (2) genetic control and environmental effects on quality traits, (3) characterization of genetic resources for quality improvement, and (4) diversifying grain properties for novel uses.

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Genetic diversity and molecular characterization of puroindoline genes ( Pina-D1 and Pinb-D1 ) in bread wheat landraces from Andalusia (Southern Spain)

Cited by 9 publications

References 24 publications

The Variability of Puroindoline-Encoding Alleles and Their Influence on Grain Hardness in Modern Wheat Cultivars Cultivated in Poland, Breeding Lines and Polish Old Landraces (Triticum aestivum L.)

The Variability of Puroindoline-Encoding Alleles and Their Influence on Grain Hardness in Modern Wheat Cultivars Cultivated in Poland, Breeding Lines and Polish Old Landraces (Triticum aestivum L.)

Exploring the End-Use Quality Potential of a Collection of Spanish Bread Wheat Landraces

Genetic improvement of wheat grain quality at CIMMYT

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