Genetic diversity and molecular epidemiology of respiratory syncytial virus over four consecutive seasons in South Africa: identification of new subgroup A and B genotypes

Venter, Marietjie; Madhi, Shabir А.; Tiemessen, Caroline T.; Schoub, Barry D.

doi:10.1099/0022-1317-82-9-2117

Cited by 217 publications

(308 citation statements)

References 31 publications

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“…However, an improvement to the protective response was not revealed by mixing. The RSV F vaccine was therefore advanced as the preferred vaccine, given that the F gene is better conserved among natural RSV isolates than is G [39][40][41][42]. To determine if that conservation was supportive of cross-neutralization and cross-protection, we tested the rSV-RSV-F construct for elicitation of neutralizing and protective responses against non-homologous challenge viruses.…”

Section: Discussionmentioning

confidence: 99%

Respiratory syncytial virus (RSV) fusion protein expressed by recombinant Sendai virus elicits B-cell and T-cell responses in cotton rats and confers protection against RSV subtypes A and B

Zhan

Hurwitz

Krishnamurthy

et al. 2007

Vaccine

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The respiratory syncytial virus (RSV) is a serious pediatric pathogen for which there is currently no clinically-approved vaccine. This report describes the design and testing of a new RSV vaccine construct (rSV-RSV-F), created by the recombination of an RSV F sequence with the murine parainfluenza virus type 1 (Sendai virus, SV) genome. SV was selected as the vaccine backbone for this study, because it has previously been shown to elicit high-magnitude, durable immune activities in animal studies and has advanced to human safety trials as a xenogenic vaccine for human parainfluenza virus-type 1 (hPIV-1). Cells infected with the recombinant SV expressed RSV F protein, but F was not incorporated into progeny SV virions. When cotton rats were inoculated with the vaccine, high-titer RSV-binding and neutralizing antibodies were induced as well as interferon-γ-producing T-cells. Most striking was the protection against intra-nasal RSV challenge conferred by the vaccine. The rSV-RSV-F construct was also tested as a mixture with a second SV construct expressing the RSV G protein, but no clear advantage was demonstrated by combining the two vaccines. As a final analysis, the efficacy of the rSV-RSV-F vaccine was tested against an array of RSV isolates. Results showed that neutralizing and protective responses were effective against RSV isolates of both A and B subtypes. Together, experimental results encourage promotion of this recombinant SV construct as a vaccine candidate for the prevention of RSV in humans.

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Section: Discussionmentioning

confidence: 99%

Respiratory syncytial virus (RSV) fusion protein expressed by recombinant Sendai virus elicits B-cell and T-cell responses in cotton rats and confers protection against RSV subtypes A and B

Zhan

Hurwitz

Krishnamurthy

et al. 2007

Vaccine

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show abstract

“…The majority of the viruses (152) belonged to subgroup B and could be further divided into two known genotypes, GB3 (137) and genotype SAB1 (15; Figs. 1 and 2) [Venter et al, 2001[Venter et al, , 2002. However, it is not known if the 152 viruses designated as subgroup B could be further divided with regard to the 60 nucleotide duplication in the C-terminal part of the G gene as described elsewhere [Trento et al, 2003] since this part of the G gene was not sequenced.…”

Section: Subgroups and Genotypesmentioning

confidence: 99%

“…The G gene consists of two variable parts, the N-terminal and the C-terminal, and a central, conserved region [Johnson et al, 1987;Cane et al, 1991;Sullender et al, 1991;Sullender, 2000;Cane, 2001]. Most frequently, genotype classification of RSV makes use of the C-terminal region of the G gene [Peret et al, 1998[Peret et al, , 2000Venter et al, 2001;Zlateva et al, 2005]. Accordingly, most studies of RSV phylogeny are based on the nucleotide variation of the C-terminal part of the G-gene [Peret et al, 1998[Peret et al, , 2000Venter et al, 2001Venter et al, , 2002Madhi et al, 2003;Sato et al, 2005].…”

Section: Introductionmentioning

confidence: 99%

“…Most frequently, genotype classification of RSV makes use of the C-terminal region of the G gene [Peret et al, 1998[Peret et al, , 2000Venter et al, 2001;Zlateva et al, 2005]. Accordingly, most studies of RSV phylogeny are based on the nucleotide variation of the C-terminal part of the G-gene [Peret et al, 1998[Peret et al, , 2000Venter et al, 2001Venter et al, , 2002Madhi et al, 2003;Sato et al, 2005]. It is only possible to differentiate new strains by sequencing the C-terminal, such as the RSV subgroup B strains with the 60 nucleotide duplication [Trento et al, 2003].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular epidemiology and genetic variability of respiratory syncytial virus (RSV) in Stockholm, 2002–2003

Östlund¹,

Lindell²,

Stenler³

et al. 2007

Journal of Medical Virology

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The epidemiology and genetic variability of circulating respiratory syncytial virus (RSV) strains in Stockholm during the season 2002-2003 were studied in consecutive RSV isolates derived from respiratory samples and diagnosed in the laboratory. Two hundred thirty-four viruses were sequenced. The samples were mainly from children under 1 year old (79%). The phylogeny of the N-terminal part of the G gene was studied after amplification and sequencing. One hundred fifty-two viruses belonged to subgroup B and 82 to subgroup A. The subgroup A viruses could be further divided into genotypes GA2 (25) and GA5 (57) and the subgroup B viruses into GB3 (137) and SAB1 (15) strains. These strains clustered with subgroup A and subgroup B strains from Kenya from the same period, as well as with strains from Great Britain from 1995 to 1998. The dominance of subgroup B strains in Stockholm during 2002-2003 is in agreement with findings from other parts of the world during the same years. Only two genotypes of subgroup A, GA2 and GA5, were circulating during this time, and GA2 has been circulating in Sweden for more than 20 years. Consecutive strains from the same individual displayed no variability in the sequenced region, which was also true of strains that had been passaged in cell cultures.

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“…The G protein is one of the targets of host immune response and thus a vaccine candidate. hRSV group B strains have been classified into 13 reported genotypes GB1, GB2, GB3, GB4, SAB1, SAB2, SAB3, SAB4, URU1, URU2, JAB1, CB1 and BA [5,8,9,21,23,25]. The BA genotype has 60 nucleotide duplication in the second hypervariable region of the G protein gene.…”

Section: Introductionmentioning

confidence: 99%

Retrospective phylogenetic analysis of circulating BA genotype of human respiratory syncytial virus with 60 bp duplication from New Delhi, India during 2007–2010

Khan

Deeba

et al. 2015

VirusDis.

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Human respiratory syncytial virus (hRSV) is the most common viral pathogen of acute lower respiratory tract infection in infants and young children. The G protein of hRSV is the trans-membrane glycoprotein that is involved in the attachment of virion with the host cell. The nasopharyngeal aspirates were subjected to RT-PCR for the second hypervariable region of the G protein gene in the present investigation. Sequencing and phylogenetic analysis revealed that all the study strains clustered within the BA genotype. The study sequences further clustered in BA-9, BA-7, BA-10 and BA-12 subgroups within the BA genotype. The G proteins of the study sequences were predicted to encode 312 and 319 amino acids. Three different N-linked glycosylation sites were observed in the deduced 93-100 amino acid region. There were 40-43 serine and threonine residues that are the potential O-linked glycosylation sites. The non-synonymous/synonymous (dN/dS) ratio was less than one indicating negative selection pressure for amino acid change in the analyzed region of the G protein. The present investigation provides information on circulating strains of BA genotype from New Delhi, India. Further elaborate investigations of the BA viruses from different regions of the world will establish the basis of the rapid global spread and evolutionary pattern of this expanding genotype.

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Genetic diversity and molecular epidemiology of respiratory syncytial virus over four consecutive seasons in South Africa: identification of new subgroup A and B genotypes

Cited by 217 publications

References 31 publications

Respiratory syncytial virus (RSV) fusion protein expressed by recombinant Sendai virus elicits B-cell and T-cell responses in cotton rats and confers protection against RSV subtypes A and B

Respiratory syncytial virus (RSV) fusion protein expressed by recombinant Sendai virus elicits B-cell and T-cell responses in cotton rats and confers protection against RSV subtypes A and B

Molecular epidemiology and genetic variability of respiratory syncytial virus (RSV) in Stockholm, 2002–2003

Retrospective phylogenetic analysis of circulating BA genotype of human respiratory syncytial virus with 60 bp duplication from New Delhi, India during 2007–2010

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