Genetic diversity and mycotoxin production of Fusarium lactis species complex isolates from sweet pepper

Poucke, Kris Van; Monbaliu, Sofie; Munaut, Françoise; Heungens, Kurt; Saeger, Sarah De; Hove, François Van

doi:10.1016/j.ijfoodmicro.2011.10.011

Cited by 24 publications

(21 citation statements)

References 42 publications

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“…Molecular data in combination with distinctive morphological characters usually have been used to distinguish new species of Fusarium (13,28,29). Loci used for phylogenetic analyses in Fusarium include nuclear ribosomal DNA (rDNA) internal transcribed spacer (ITS; 19), translation elongation factor 1-α (EF-1α; 21,22), ribosomal intergenic spacer (IGS; 16,20), mitochondrial small subunit (mtSSU) rDNA (14), the second largest subunit of the RNA polymerase II (29), the β-tubulin gene, and the calmodulin gene (28,38).…”

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confidence: 99%

Identification and Characterization of Fusarium Species Associated with Wilt of Eleocharis dulcis (Chinese water chestnut) in China

Zhu

Zheng

et al. 2014

Plant Disease

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Zhu, Z., Zheng, L., Pan, L., Hsiang, T., and Huang, J. 2014. Identification and characterization of Fusarium species associated with wilt of Eleocharis dulcis (Chinese water chestnut) in China. Plant Dis. 98:977-987.Fusarium wilt is one of the most important diseases of Eleocharis dulcis (Chinese water chestnut) in China. In order to characterize the pathogens responsible, 69 Fusarium isolates were collected from diseased plants in E. dulcis production areas of the Chinese provinces Anhui, Fujian, Hubei, Hunan, Jiangsu, and Zhejiang. These were then identified based on morphological and molecular characteristics. F. commune was the most common species (92.8%) and was widely distributed in the six provinces. A novel species within the Gibberella fujikuroi species complex (GFSC) was found in Hubei and Zhejiang provinces (5.8%), and an unidentified Fusarium sp. was found only in Hubei province (1.4%). Thirty F. commune isolates from different provinces and four GFSC isolates were selected for sequence analyses of the translation elongation factor 1-α (EF-1α), the mitochondrial small subunit (mtSSU) ribosomal DNA, and the nuclear ribosomal intergenic spacer region (IGS). Maximum parsimony and Bayesian analyses of the multilocus sequence data of these two species plus other taxa showed that the two species formed two distinct, well-supported clades among the three individual and combined gene genealogies. Isolates from different locations were scattered, with no evidence of geographic specialization. Pathogenicity assays showed that the two Fusarium spp., including the unidentified Fusarium sp., were pathogenic to E. dulcis 'Tuanfeng seven'. There was no relationship between the source of isolates and their pathogenicity. This is the first description of F. commune, a novel species within the GFSC, and an unidentified Fusarium sp. as causal agents of Fusarium wilt of E. dulcis in China.

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Identification and Characterization of Fusarium Species Associated with Wilt of Eleocharis dulcis (Chinese water chestnut) in China

Zhu

Zheng

et al. 2014

Plant Disease

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“…Fumonisins are associated with carcinogenic effects of the esophagus and liver (Shephard ). Van Poucke et al () isolated several Fusarium spp. from paprika powder.…”

Section: Resultsmentioning

confidence: 99%

“…Exposure to these mycotoxins always result in gastrointestinal effects (fumonisin and DON) or growth retardation in animals (RFQ). Fumonisins are associated with carcinogenic effects of the esophagus and liver(Shephard 2011) Van Poucke et al (2012). isolated several Fusarium spp.…”

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Qualitative Detection of Fungal Contamination in Paprika Powder

Vyhnánek

Machálková

Trojan

et al. 2016

Journal of Food Safety

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Dried red pepper is one of the most commonly used spices in many parts of the world. In this study molecular biology methods were applied for detection of contamination in nine samples of paprika powder. Internal Transcribed Spacer (ITS) regions were selected for sequencing as they have a high variability between species and organisms and therefore are an appropriate tool for taxonomic identification. The sequence analysis of the ITS regions were identified by high sequence similarity with the ITS regions of many microscopic fungi, especially representatives of the class Ascomycota and several other yeast species. This work was a qualitative rather than a quantitative detection, so the extent to which fungi and yeasts were present in the samples is unknown. However, supplied quality certificates (microbiological data) indicated the overall quality of samples. Harmful microorganisms were identified in the paprika powder samples and the likely mode of contamination was identified. Practical Application The presence of undesirable pathogens can be a serious problem for consumers, especially contamination by microscopic fungi that have the potential to produce mycotoxins. Today's conventional methods for determining contamination by microorganisms are time consuming and can be difficult to identify some less common types of pathogens. Fungal DNA (ITS regions) sequences of the class Ascomycetes were identified from samples of sweet and hot paprika powder.

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“…The identities of C. truncatum and C. brevisporum cultures were confirmed using comparisons of multiple gene sequences (internally transcribed spacer region, ITS1-5.8S-ITS2; beta tubulin, TUB2; Actin, ACT-GenBank Accession Nos. MG822830-2, KJ780718, KR029613, MG827234-5, MG839690-1, MG870320-1) after PCR amplification of total genomic DNA extracted from each fungal culture according to published protocols (van Poucke et al, 2012;White, Bruns, Lee, & Taylor, 1990).…”

Section: Isolation Of Fungal Pathogensmentioning

confidence: 99%

Diversity, structure, and synteny of the cutinase gene ofColletotrichumspecies

Villafana

Rampersad

2020

Ecology and Evolution

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Colletotrichum species complexes are among the top 10 economically important fungal plant pathogens worldwide because they can infect climacteric and nonclimacteric fruit at the pre and/or postharvest stages. C. truncatum is the major pathogen responsible for anthracnose of green and red bell pepper fruit worldwide. C. brevisporum was recently reported to be a minor pathogen of red bell pepper fruit in Trinidad, but has recently been reported as pathogenic to other host species in other countries. The ability of these phytopathogens to produce and secrete cutinase is required for dismantling the cuticle of the host plant and, therefore, crucial to the necrotrophic phase of their infection strategy. In vitro bioassays using different lipid substrates confirmed the ability of C. truncatum and C. brevisporum isolates from green and red bell peppers to secrete cutinase. The diversity, structure and organization and synteny of the cutinase gene were determined among different Colletotrichum species. Cluster analysis indicated a low level of nucleotide variation among C. truncatum sequences. Nucleotide sequences of C. brevisporum were more related to C. truncatum cutinase nucleotide sequences than to C. gloeosporioides. Cluster patterns coincided with haplotype and there was evidence of significant positive selection with no recombination signatures. The structure of the cutinase gene included two exons with one intervening intron and, therefore, one splice variant. Although amino acid sequences were highly conserved among C. truncatum isolates, diversity “hot spots” were revealed when the 66‐amino acid coding region of 200 fungal species was compared. Twenty cutinase orthologues were detected among different fungal species, whose common ancestor is Pezizomycotina and it is purported that these orthologues arose through a single gene duplication event prior to speciation. The cutinase domain was retained both in structure and arrangement among 34 different Colletotrichum species. The order of aligned genomic blocks between species and the arrangement of flanking protein domains were also conserved and shared for those domains immediately located at the N‐ and C‐terminus of the cutinase domain. Among these were an RNA recognition motif, translation elongation factor, signal peptide, pentatricopeptide repeat, and Hsp70 family of chaperone proteins, all of which support the expression of the cutinase gene. The findings of this study are important to understanding the evolution of the cutinase gene in C. truncatum as a key component of the biotrophic–necrotrophic switch which may be useful in developing gene‐targeting strategies to decrease the pathogenic potential of Colletotrichum species.

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Genetic diversity and mycotoxin production of Fusarium lactis species complex isolates from sweet pepper

Cited by 24 publications

References 42 publications

Identification and Characterization of Fusarium Species Associated with Wilt of Eleocharis dulcis (Chinese water chestnut) in China

Identification and Characterization of Fusarium Species Associated with Wilt of Eleocharis dulcis (Chinese water chestnut) in China

Qualitative Detection of Fungal Contamination in Paprika Powder

Diversity, structure, and synteny of the cutinase gene ofColletotrichumspecies

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