Genetic diversity and pathogenicity of <i>Monilinia polystroma</i> – the new pathogen of cherries

Poniatowska, Anna; Michalecka, Monika; Puławska, Joanna

doi:10.1111/ppa.12463

Cited by 13 publications

(13 citation statements)

References 30 publications

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“…Contrary to this, Mo. polystroma , which is a cherry pathogen ( Poniatowska et al, 2016 ), was detected only after both fungicide application. In this case, these type of contradictory results made very difficult to establish some clear impact of agronomic treatments on microbial communities of musts.…”

Section: Discussionmentioning

confidence: 62%

Impact of Chemical and Biological Fungicides Applied to Grapevine on Grape Biofilm, Must, and Wine Microbial Diversity

et al. 2018

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This study was aimed to measure the impact of the application of a bio-fungicide against Botrytis cinerea on the microbiota involved in the alcoholic fermentation (AF) of Tempranillo Rioja wines. For this purpose, a bio-fungicide composed of the biological control bacterium Bacillus subtilis QST713 was applied to the vineyard. The microbial diversity was analyzed from grape biofilm to wine. Impact on microbial diversity was measured employing indexes assessed with the software PAST 3.10 P.D. Results were compared to non-treated samples and to samples treated with a chemical fungicide mainly composed by fenhexamid. Overall, the impact of the biological-fungicide (bio-fungicide) on the microbial diversity assessed for grape biofilm and for musts was not remarkable. Neither of the tested fungicides enhanced the growth of any species or acted against the development of any microbial groups. The bio-fungicide had no significant impact on the wine microbiota whereas the chemical fungicide caused a reduction of microbial community richness and diversity. Although environmental threats might generate a detriment of the microbial species richness, in this study the tested bio-fungicide did not modify the structure of the microbial community. Indeed, some of the Bacillus applied at the grape surface, were detected at the end of the AF showing its resilience to the harsh environment of the winemaking; in contrast, its impact on wine quality during aging is yet unknown.

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Section: Discussionmentioning

confidence: 62%

Impact of Chemical and Biological Fungicides Applied to Grapevine on Grape Biofilm, Must, and Wine Microbial Diversity

et al. 2018

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“…A slightly modified method of PCR MP (Masny & Płucienniczak, 2003;Poniatowska et al, 2015) was used for the characterization and differentiation of the isolates. The digestion of 100 ng DNA with NcoI (10 U lL À1 ; Thermo Scientific) was performed overnight at 37°C according to the manufacturer's instructions.…”

Section: Pcr Melting Profilementioning

confidence: 99%

Phenotypic and genetic characterization of Pseudomonas syringae strains associated with the recent citrus bacterial blast and bacterial black pit epidemics in Tunisia

Abdellatif¹,

Kałużna

Janse

et al. 2017

Plant Pathology

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In the spring of 2012, symptoms of a disease resembling citrus blast and citrus black pit were observed in some orchards in Tunisia. The epidemic spread rapidly in the following years. Twenty‐four commercial citrus orchards from four Tunisian regions showing characteristic symptoms of bacterial diseases were surveyed during a 3‐year study. Eighty‐eight Pseudomonas‐like bacterial isolates were successfully obtained from the northeast and west of Tunisia. No isolates were recovered from the central region. Overall, 46 isolates were identified as Pseudomonas syringae pv. syringae and most of them showed similar phenotypic and genetic profiles. The virulence of three selected isolates differed from one plant cultivar to another as well as from the type of plant organ used for the inoculation. In a bioassay test, all isolates produced syringomycin, which was confirmed by molecular detection based on the syrB and syrD genes. Only EC122 possessed syrD but not syrB. DNA fingerprints, based on repetitive sequence‐based polymerase chain reaction (rep‐PCR) and PCR melting profile (PCR MP), were used to determine the potential genetic diversity among strains. Clustering of PCR MP fingerprinting data matched with rep‐PCR fingerprinting data. The generated distribution tree showed that Tunisian isolates were closely related to the citrus reference strain LMG5496. In contrast, EC112, isolated from citrus, and the almond isolate EC122 were distantly related to the type strain LMG1247T isolated from lilac. Such studies have not been reported until now for P. syringae from citrus.

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“…A similar tendency was observed for M . polystroma strains in the dendrograms constructed on the basis of PCR MP, ISSR, and RAPD results (Poniatowska et al, 2016) and for strains belonging to different Monilinia spp. analysed by using a multigene phylogeny based on g3pdh and tub2 sequences (Yin et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Some M . polystroma isolates have been identified in earlier studies based on their genetic features (Poniatowska et al, 2016; Table ).…”

Section: Methodsmentioning

confidence: 99%

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Phylogenetic relationships and genetic diversity of Monilinia spp. isolated in Poland based on housekeeping‐ and pathogenicity‐related gene sequence analysis

2021

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Monilinia spp. fungi, which cause brown rot disease, are of great economic importance for pome and stone fruit production in Poland. To date, four species of this genus, M. fructicola, M. fructigena, M. laxa, and M. polystroma, have been identified in this country. However, little is known about their phylogenetic relationships and genetic diversity. In this research, β‐tubulin and three genes not previously studied in these species, heat shock protein 60 (hsp60), acid protease 1 (acp1), and a zinc finger transcription factor (pac1), were investigated to infer the phylogenetic relationships among these species. Diversity data, as well as recombination analyses of the sequenced regions, were used to assess the genetic variation of Monilinia fungi and the evolutionary forces that are currently acting upon these microorganisms. Based on the topology of the obtained dendrograms, the current study demonstrates the lack of a relationship between the phylogenetic position of the strain, the host plant, and the geographical origin of the strain. Additionally, our study provided evidence that phylogenetic analysis using a combination of tub2, hsp60, acp1, and pac1 gene sequences can be used to effectively resolve the relationships among Monilinia species. For each species analysed in this study, low genetic diversity and a lack of recombination were detected. On the basis of the obtained results, it can be concluded that variation in the population of Monilinia spp. in Poland is largely influenced by mutation rather than recombination processes.

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Genetic diversity and pathogenicity of Monilinia polystroma – the new pathogen of cherries

Cited by 13 publications

References 30 publications

Impact of Chemical and Biological Fungicides Applied to Grapevine on Grape Biofilm, Must, and Wine Microbial Diversity

Impact of Chemical and Biological Fungicides Applied to Grapevine on Grape Biofilm, Must, and Wine Microbial Diversity

Phenotypic and genetic characterization of Pseudomonas syringae strains associated with the recent citrus bacterial blast and bacterial black pit epidemics in Tunisia

Phylogenetic relationships and genetic diversity of Monilinia spp. isolated in Poland based on housekeeping‐ and pathogenicity‐related gene sequence analysis

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