Genetic diversity and phylogeny of strains of <i>Clavibacter nebraskensis</i> associated with recent and historic Goss's wilt epidemics in the north Central USA

Webster, B. T.; Curland, Rebecca D.; Hirsch, Cory D.; McNally, R. R.; Malvick, Dean; Ishimaru, Carol A.

doi:10.1111/ppa.13185

Cited by 7 publications

(7 citation statements)

References 38 publications

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“…This protein may be unique to this species because the activity is not found in culture supernatants from C. michiganensis. The formation of membrane channels is a common mode of action for toxins, thus this protein could be a toxin involved in pathogenesis (Metzler et al, 1997;Schürholz et al, 1991) Strains of C. nebraskensis vary in aggressiveness on maize (Soliman et al, 2021), and virulence or avirulence in maize is not correlated with a particular MLSA-based phylogeny (Webster et al, 2020). Among 33 candidate virulence genes, sequence polymorphisms were found in only five genes: cellulase A, two endoglucanases, xylanase B, and a pectate lyase (Ahmad et al, 2015).…”

Section: Pathog Enicit Y Mechanis Msmentioning

confidence: 99%

“…However, while sequence polymorphisms were detected in five putative virulence genes of C. nebraskensis , that is, cellulase A ( celA ), two endoglucanases, xylanase B, and a pectate lyase, there was no relationship with pathogenicity (Ahmad et al, 2015 ). Using multilocus sequence analysis and typing (MLSA/MLST), Webster et al ( 2020 ) suggested that genetic changes in the population of C. nebraskensis were correlated with recent geographic expansions of Goss's wilt outbreaks in the north‐central United States. Moreover, a significant substructure was detected between subpopulations from historic outbreaks in Nebraska and Colorado and more recent outbreaks in Minnesota.…”

Section: Genetic Diversity and Population Structurementioning

confidence: 99%

“…Moreover, a significant substructure was detected between subpopulations from historic outbreaks in Nebraska and Colorado and more recent outbreaks in Minnesota. Webster et al ( 2020 ) noted that 34% of the C. nebraskensis strains evaluated were classified as either weakly virulent or very weak virulence/avirulent, while virulence or avirulence in maize was not correlated with a particular MLSA scheme.…”

Section: Genetic Diversity and Population Structurementioning

confidence: 99%

“…Strains of C. nebraskensis vary in aggressiveness on maize (Soliman et al, 2021 ), and virulence or avirulence in maize is not correlated with a particular MLSA‐based phylogeny (Webster et al, 2020 ). Among 33 candidate virulence genes, sequence polymorphisms were found in only five genes: cellulase A, two endoglucanases, xylanase B, and a pectate lyase (Ahmad et al, 2015 ).…”

Section: Pathogenicity Mechanismsmentioning

confidence: 99%

“…Using multilocus sequence analysis and typing (MLSA/MLST),Webster et al (2020) suggested that genetic changes in the population of C. nebraskensis were correlated with recent geographic expansions of Goss's wilt outbreaks in the north-central United States. Moreover, a significant substructure was detected between subpopulations from historic outbreaks in Nebraska and Colorado and more recent outbreaks in Minnesota Webster et al (2020). noted that 34% of the C. nebraskensis strains evaluated were classified as either weakly virulent or very weak virulence/avirulent, while virulence or avirulence in maize was not correlated with a particular MLSA scheme.…”

mentioning

confidence: 99%

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Clavibacter nebraskensis causing Goss's wilt of maize: Five decades of detaining the enemy in the New World

Osdaghi

Robertson

Jackson-Ziems

et al. 2022

Molecular Plant Pathology

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Goss's bacterial wilt and leaf blight of maize (Zea mays) caused by the gram-positive coryneform bacterium Clavibacter nebraskensis is an economically important disease in North America. C. nebraskensis is included within the high-risk list of quarantine pathogens by several plant protection organizations (EPPO code: CORBMI), hence it is under strict quarantine control around the world. The causal agent was reported for the first time on maize in Nebraska (USA) in 1969. After an outbreak during the 1970s, prevalence of the disease decreased in the 1980s to the early 2000s, before the disease resurged causing a serious threat to maize production in North America.The re-emergence of Goss's wilt in the corn belt of the United States led to several novel achievements in understanding the pathogen biology and disease control. In this review, we provide an updated overview of the pathogen taxonomy, biology, and epidemiology as well as management strategies of Goss's wilt disease. First, a taxonomic history of the pathogen is provided followed by symptomology and host range, genetic diversity, and pathogenicity mechanisms of the bacterium. Then, utility of high-throughput molecular approaches in the precise detection and identification of the pathogen and the management strategies of the disease are explained. Finally, we highlight the role of integrated pest management strategies to combat the risk of Goss's wilt in the 21st century maize industry.Disease symptoms: Large (2-15 cm) tan to grey elongated oval lesions with wavy, irregular water-soaked margins on the leaves. The lesions often start at the leaf tip or are associated with wounding caused by hail or wind damage. Small (1 mm in diameter), dark, discontinuous water-soaked spots, known as "freckles", can be observed in the periphery of lesions. When backlit, the freckles appear translucent. Early infection (prior to growth stage V6) may become systemic and cause seedlings to wilt, wither, and die. Coalescence of lesions results in leaf blighting.Host range: Maize (Zea mays) is the only economic host of the pathogen. A number of Poaceae species are reported to act as secondary hosts for C. nebraskensis.

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Section: Pathog Enicit Y Mechanis Msmentioning

confidence: 99%

Section: Genetic Diversity and Population Structurementioning

confidence: 99%

Section: Genetic Diversity and Population Structurementioning

confidence: 99%

Section: Pathogenicity Mechanismsmentioning

confidence: 99%

mentioning

confidence: 99%

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Clavibacter nebraskensis causing Goss's wilt of maize: Five decades of detaining the enemy in the New World

Osdaghi

Robertson

Jackson-Ziems

et al. 2022

Molecular Plant Pathology

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Clavibacter nebraskensis (Goss's bacterial wilt and leaf blight)

Osdaghi¹

2020

CABI Compendium

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Genetic and transcriptomic dissection of host defense to Goss's bacterial wilt and leaf blight of maize

Hao,

Hu,

Jaqueth

et al. 2023

G3: Genes, Genomes, Genetics

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Goss’s wilt, caused by the Gram-positive actinobacterium Clavibacter nebraskensis, is an important bacterial disease of maize. The molecular and genetic mechanisms of resistance to the bacterium, or, in general, Gram-positive bacteria causing plant diseases, remain poorly understood. Here, we examined the genetic basis of Goss’s wilt through differential gene expression, standard genome-wide association mapping (GWAS), extreme phenotype (XP) GWAS using highly resistant (R) and highly susceptible (S) lines, and quantitative trait locus (QTL) mapping using three bi-parental populations, identifying eleven disease association loci. Three loci were validated using near-isogenic lines or recombinant inbred lines. Our analysis indicates that Goss’s wilt resistance is highly complex and major resistance genes are not commonly present. RNA sequencing of samples separately pooled from R and S lines with or without bacterial inoculation was performed, enabling identification of common and differential gene responses in R and S lines. Based on expression, in both R and S lines, the photosynthesis pathway was silenced upon infection, while stress-responsive pathways and phytohormone pathways, namely, abscisic acid, auxin, ethylene, jasmonate, and gibberellin, were markedly activated. In addition, sixty-five genes showed differential responses (up- or down-regulated) to infection in R and S lines. Combining genetic mapping and transcriptional data, individual candidate genes conferring Goss’s wilt resistance were identified. Collectively, aspects of the genetic architecture of Goss’s wilt resistance were revealed, providing foundational data for mechanistic studies.

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Genetic diversity and phylogeny of strains of Clavibacter nebraskensis associated with recent and historic Goss's wilt epidemics in the north Central USA

Cited by 7 publications

References 38 publications

Clavibacter nebraskensis causing Goss's wilt of maize: Five decades of detaining the enemy in the New World

Clavibacter nebraskensis causing Goss's wilt of maize: Five decades of detaining the enemy in the New World

Clavibacter nebraskensis (Goss's bacterial wilt and leaf blight)

Genetic and transcriptomic dissection of host defense to Goss's bacterial wilt and leaf blight of maize

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