Genetic Diversity and Phylogeny of Rhizobia That Nodulate
            <i>Acacia</i>
            spp. in Morocco Assessed by Analysis of rRNA Genes

Khbaya, Bouchaib; Neyra, Marc; Normand, Philippe; Zerhari, K.; Filali‐Maltouf, Abdelkarim

doi:10.1128/aem.64.12.4912-4917.1998

Cited by 78 publications

(38 citation statements)

References 50 publications

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“…The fact that bacteria isolated from the nodules of legume plants is similar to Agrobacteria has already been reported (Tan et al 1999). Khbaya et al (1998), and also explains why some of the strains present in the nodules that resembled Agrobacterium could not nodulate the original host. However, bacteria isolated from Caragana intermedia could induce nodule formation in hosts after infected Caragana intermedia for one month.…”

Section: Discussionmentioning

confidence: 67%

Genetic diversity of rhizobia isolated from Caragana intermedia in Maowusu sandland, north of China

Gao

Wang

2002

Lett Appl Microbiol

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Aims: Thirty-three rhizobial strains isolated from nodules of Caragana intermedia in Maowusu sandland were examined for their genetic diversity and putative phylogenetic position. Methods and Results: Isolates from Caragana intermedia were classified into 12 genotypes by 16S rDNA polymerase chain reaction-restriction fragment length polymorphism (RFLP), which showed no distinct relationships with those of the reference strains. The genotypes of rhizobia were not related to geographical location. Thr 16S rDNA sequence of representative strain GH2001 from dominant genotype 2 shared high homologuey with some Rhizobium species: Rh. giardinii (96AE4%), Rh. huautlense (95AE3%), Rh. galegae (95AE7%), Rh. yanglingense (95AE2%), Rh. mongolense (95AE6%), Rh. radiobacter (99%) and Rh. rubi (98AE3%). Conclusions: A high degree of genetic diversity existed among rhizobia nodulating Caragana intermedia in Maowusu sandland. Most of the new isolates might belong to Rhizobium. Significance and Impact of the Study: The results suggest that the rich diversity of rhizobia might have contributed to the adaptation of the arid region. These strains could be valuable at the economic and ecosystem level.

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Section: Discussionmentioning

confidence: 67%

Genetic diversity of rhizobia isolated from Caragana intermedia in Maowusu sandland, north of China

Gao

Wang

2002

Lett Appl Microbiol

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“…This strain could be useful for the inoculation and improvement of A. seyal-rhizobium symbiosis in salt-affected soils (Diouf et al 2005). African acacias are predominantly nodulated by Mesorhizobium, Rhizobium, or Ensifer genera (Haukka et al 1998;Khbaya et al 1998;de Lajudie et al 1998;Ba et al 2002;Sarr et al 2005;Diouf et al 2007 Figure 4 Phylogenetic (neighbour-joining) tree based on 362 nt aligned sequences of nifH gene using MEGA4. The isolation hosts are indicated within brackets.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analyses of symbiotic genes and characterization of functional traits of Mesorhizobium spp. strains associated with the promiscuous species Acacia seyal Del.

Diouf

Fall

Chaintreuil

et al. 2010

Journal of Applied Microbiology

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Aims: To assess the phenotypic, symbiotic and genotypic diversity scope of Mesorhizobium spp. strains associated with Acacia seyal (Del.) isolated from different agro‐ecological zones in Senegal, and uses of susceptible microbial inoculum in a reafforestation process. Methods and Results: A polyphasic approach including phenotypic and genotypic techniques was used to study the diversity and their relationships with other biovars and species of rhizobia. The geographical origins of the strains have limited effect on their phylogenetic and phenotypic classification. Nodulation tests indicated promiscuity of the strains studied, because they were capable of nodulating six woody legume species (Acacia auriculiformis, Acacia senegal, A. seyal, Acacia tortilis ssp. raddiana, Leucaena leucocephala and Prosopis juliflora). Sequencing and phylogenetic analyses of nodA, nodC and nifH genes pointed out that in contrast to nodA gene, the phylogenies of nodC and nifH genes were not consistent with that of 16S rRNA, indicating that these genes of the A. seyal‐nodulating rhizobia might have different origins. Microbial inoculation on nonsterile soil had significant effect on the nodules number and the growth of the seedlings, indicating that these strains of rhizobia might be used as inoculum. Conclusions: The results indicated that A. seyal is a nonselective host that can establish effective symbiosis with Mesorhizobium spp. strains from diverse genomic backgrounds and that the selected A. seyal‐nodulating rhizobia could enhance plant growth. Significance and Impact of the Study: These results showed the important role that A. seyal could play in the improvement of reafforestation process as a promiscuous host, which can establish effective symbiosis with rhizobia from diverse genomic backgrounds.

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“…The length of the ampli®ed region, which included almost the entire 16S RNA gene (1500 bp; Willems and Collins 1993), the IGS and about 100 bp of the 23S rDNA, was different among the S. junceum isolates and also among the reference strains ( Fig. 1 and Table 1) as expected (Laguerre et al 1996;Khbaya et al 1998).…”

Section: S±igs Rdna Analysismentioning

confidence: 72%

“…The combination of the 16S rRNA gene and 16S±23S IGS generated a long and variable (Fig. 1) PCR fragment whose size increased the potential to differentiate between close strains (Nour et al 1994a;Gu Èrtler and Stanisich 1996;Laguerre et al 1996;Normand et al 1996;Selenska-Pobell et al 1996;Khbaya et al 1998;de Oliveira et al 1999).…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, 16S rDNA is a rather conserved molecule and it may show its limitations when attempting to discriminate closely related species (Young and Haukka 1996). As an alternative, length and sequence heterogeneity of the intergenic spacer (IGS) between the 16S and 23S rRNA genes may be effective markers for detecting genetic differences at both the intergeneric and interspeci®c level (Navarro et al 1992;Nour et al 1994a;Nour et al 1994b;Gu Èrtler and Stanisich 1996;Laguerre et al 1996;Normand et al 1996;Selenska-Pobell et al 1996;Khbaya et al 1998;de Oliveira et al 1999). Therefore, we combined the two levels of information, focusing on both the 16S and IGS regions of a group of rhizobia isolated from S. junceum collected in Sicily.…”

Section: Introductionmentioning

confidence: 99%

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Bradyrhizobium sp. nodulating the Mediterranean shrub Spanish broom (Spartium junceum L.)

et al. 2002

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Aims: The molecular diversity of 25 strains of rhizobia, isolated in Sicily from root nodules of the Mediterranean shrubby legume Spanish broom (Spartium junceum L.), is presented in relation to the known rhizobial reference strains. Methods and Results: Our approach to the study of the S. junceum rhizobial diversity combined the information given by the 16S and the intergenic spacer (IGS) 16S±23S rDNA polymorphic region by obtaining them in a single polymerase chain reaction (PCR) step. The PCR fragment size of the S. junceum isolates was 2400±2500 bp and that of the reference strains varied from 2400 in Bradyrhizobium strains to 2800 in Sinorhizobium strains. Inter-and intrageneric length variability was found among the reference strains. Restriction fragment length polymorphisms (RFLP) analysis allowed us to identify eight genotypes among the S. junceum rhizobia that were clustered into two groups, both related to the Bradyrhizobium lineage. Sequencing of representative strains of the two clusters con®rmed these data. The 16S±IGS PCR±RFLP approach, when applied to rhizobial reference strains, allowed very close species (i.e. Rhizobium leguminosarum/R. tropici) to be separated with any of the three enzymes used; however, cluster analysis revealed inconsistencies with the 16S-based phylogenesis of rhizobia. Conclusions: Rhizobia nodulating S. junceum in the Mediterranean region belong to the Bradyrhizobium lineage. Our results con®rm the resolution power of the 16S±23S rDNA in distinguishing among rhizobia genera and species, as well as the usefulness of the PCR±RFLP method applied to the entire 16S±IGS region for a rapid tracking of the known relatives of new isolates. Signi®cance and Impact of the Study: The present paper is, to our knowledge, the ®rst report on rhizobia nodulating a Mediterranean wild woody legume.

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Genetic Diversity and Phylogeny of Rhizobia That Nodulate Acacia spp. in Morocco Assessed by Analysis of rRNA Genes

Cited by 78 publications

References 50 publications

Genetic diversity of rhizobia isolated from Caragana intermedia in Maowusu sandland, north of China

Genetic diversity of rhizobia isolated from Caragana intermedia in Maowusu sandland, north of China

Phylogenetic analyses of symbiotic genes and characterization of functional traits of Mesorhizobium spp. strains associated with the promiscuous species Acacia seyal Del.

Bradyrhizobium sp. nodulating the Mediterranean shrub Spanish broom (Spartium junceum L.)

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