Genetic diversity and population dynamics of hyphomicrobium spp. in a sewage treatment plant and its receiving lake

Fesefeldt, Andreas; Holm, Niels C.; Gliesche, Christian G.

doi:10.2166/wst.1998.0597

Cited by 6 publications

(3 citation statements)

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“…This finding suggests that the isolates could be maintained in the mixed population of microorganisms found in sludge as is observed with P. denitrificans, which reduces NO 3 Ϫ more efficiently than a control reactor without the strain when it is mixed with activated sludge (9). Sometimes, a C 1 carbon source, such as methanol or formate, has been used to manipulate the dominant bacterial species in sludge (5,21). Our novel strains should be useful for constructing new aerobic denitrification processes that do not produce N 2 O, since these strains could be selected as the dominant organisms by using C 1 compounds as the electron donors.…”

Section: Discussionmentioning

confidence: 94%

Aerobic Denitrifying Bacteria That Produce Low Levels of Nitrous Oxide

Takaya

Catalan-Sakairi

Sakaguchi

et al. 2003

Appl Environ Microbiol

278

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Most denitrifiers produce nitrous oxide (N 2 O) instead of dinitrogen (N 2 ) under aerobic conditions. We isolated and characterized novel aerobic denitrifiers that produce low levels of N 2 O under aerobic conditions. We monitored the denitrification activities of two of the isolates, strains TR2 and K50, in batch and continuous cultures. Both strains reduced nitrate (NO 3 ؊ ) to N 2 at rates of 0.9 and 0.03 mol min ؊1 unit of optical density at 540 nm ؊1 at dissolved oxygen (O 2 ) (DO) concentrations of 39 and 38 mol liter ؊1 , respectively. At the same DO level, the typical denitrifier Pseudomonas stutzeri and the previously described aerobic denitrifier Paracoccus denitrificans did not produce N 2 but evolved more than 10-fold more N 2 O than strains TR2 and K50 evolved. The isolates denitrified NO 3؊ with concomitant consumption of O 2 . These results indicated that strains TR2 and K50 are aerobic denitrifiers. These two isolates were taxonomically placed in the ␤ subclass of the class Proteobacteria and were identified as P. stutzeri TR2 and Pseudomonas sp. strain K50. These strains should be useful for future investigations of the mechanisms of denitrifying bacteria that regulate N 2 O emission, the single-stage process for nitrogen removal, and microbial N 2 O emission into the ecosystem.Nitrous oxide (N 2 O) is a gaseous nitrogen oxide that is present at a concentration of about 350 ppb in the atmosphere. The concentration of this compound was maintained below 300 ppb in the global nitrogen cycle before the 20th century. However, recent reports suggest that the atmospheric concentration of N 2 O is now increasing at a rate as high as 0.3% per year (1). N 2 O has a 200-to 300-fold-stronger greenhouse effect than carbon dioxide (CO 2 ) and has the potential to destroy the ozone layer (17). Therefore, the N 2 O balance is critical to the natural environment. The proposed sources of N 2 O are chemical industries, burning fossil fuels, and biomass, as well as soil denitrification of nitrogenous compounds resulting from excess agricultural fertilizer (3,6,25). Another critical source of N 2 O is wastewater treatment plants, in which considerable amounts of nitrogen pollutants removed from treated water are released into the atmosphere as N 2 O, as well as dinitrogen (N 2 ).Currently, nitrogen removal in wastewater treatment plants is essentially based on the activity of nitrifying and denitrifying microorganisms, both of which are inhabitants of activated sludge. Nitrifying bacteria aerobically oxidize ammonium contaminants to nitrite (NO 2 Ϫ ) and nitrate (NO 3 Ϫ ), which are then reduced by denitrifying bacteria to gaseous nitrogen forms such as N 2 O and N 2 . Efficient wastewater treatment relies on successively exposing water to aerobic and anaerobic conditions, since nitrification and denitrification are aerobic and anaerobic processes, respectively (4, 18). These properties represent a shortcoming of current systems since both denitrification and nitrification produce N 2 O as a by-product in the absen...

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Section: Discussionmentioning

confidence: 94%

Aerobic Denitrifying Bacteria That Produce Low Levels of Nitrous Oxide

Takaya

Catalan-Sakairi

Sakaguchi

et al. 2003

Appl Environ Microbiol

278

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“…Denaturing gradient gel electrophoresis (DGGE) has been described for detection of alternate gene forms found in environmental samples (Fesefeldt et al 1998;Rosado et al 1998;Henckel et al 2000). Accordingly, ORF13-PCR products from DNA extracted from swine waste or microcosms inoculated with either E. faecalis CG110 or swine waste were analyzed by using the DGGE technique.…”

Section: Dggementioning

confidence: 99%

Survival of enterococci and Tn916-like conjugative transposons in soil

Andrews

Johnson²,

Guard³

et al. 2004

Can. J. Microbiol.

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The persistence of Enterococcus faecalis, fecal enterococci from swine waste, and Tn916-like elements was determined following inoculation into autoclaved and native soil microcosms. When cells of E. faecalis CG110 (Tn916) were inoculated into native microcosms, enterococcal viability in the soil decreased approximately 5 orders of magnitude (4.8 x 10(5) CFU/g soil to < 10 CFU/g) after 5 weeks. In autoclaved microcosms, the viability of E. faecalis decreased by only 20% in 5 weeks. In contrast, the content of Tn916, based on PCR of DNA extracts from soil microcosms, decreased by about 20% in both native and autoclaved microcosms. Similar results were obtained when the source of fecal enterococci and Tn916-like elements was swine waste. Because the concentration of Tn916-independent E. faecalis DNA (the D-alanine D-alanine ligase gene), based on PCR, decreased to nearly undetectable levels (at least 3 orders of magnitude) after 5 weeks in the native microcosms, the evidence suggests Tn916 stability in the soil results from en masse transfer of the transposon to the normal soil microflora and not survival of E. faecalis DNA in the soil system. Results from denaturing gradient gel electrophoresis suggest that multiple forms of Tn916 occur in swine waste, but only forms most like Tn916 exhibit stability in the soil.

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“…Another most abundant bacterial genus was Hyphomicrobium , which increased in both reactors from 3.9% to 7.1% in R1 and 2.1% to 6.4% in R2. Bacteria belonging to this genus are known to cooperate with methanogens (i.e., Methanosarcina ) in simultaneous denitrification and methanogenesis [ 41 ] and the aforementioned Romboutsia (from 5.8% up to 8.7% relative abundance) indicating a constant H 2 consumption also by homoacetogenesis. Also, members belonging to Gordonia increased in both reactors (from 3% up to 9% in R1and 3% to 8% in R2).…”

Section: Resultsmentioning

confidence: 99%

Performance Analysis and Microbial Community Evolution of In Situ Biological Biogas Upgrading with Increasing H2/CO2 Ratio

Corbellini

Chen

Bellucci

et al. 2021

Archaea

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The effect of the amount of hydrogen supplied for the in situ biological biogas upgrading was investigated by monitoring the process and evolution of the microbial community. Two parallel reactors, operated at 37°C for 211 days, were continuously fed with sewage sludge at a constant organic loading rate of 1.5 gCOD∙(L∙d)-1 and hydrogen (H2). The molar ratio of H2/CO2 was progressively increased from 0.5 : 1 to 7 : 1 to convert carbon dioxide (CO2) into biomethane via hydrogenotrophic methanogenesis. Changes in the biogas composition become statistically different above the stoichiometric H2/CO2 ratio (4 : 1). At a H2/CO2 ratio of 7 : 1, the methane content in the biogas reached 90%, without adversely affecting degradation of the organic matter. The possibility of selecting, adapting, and enriching the original biomass with target-oriented microorganisms able to biologically convert CO2 into methane was verified: high throughput sequencing of 16S rRNA gene revealed that hydrogenotrophic methanogens, belonging to Methanolinea and Methanobacterium genera, were dominant. Based on the outcomes of this study, further optimization and engineering of this process is feasible and needed as a means to boost energy recovery from sludge treatment.

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Genetic diversity and population dynamics of hyphomicrobium spp. in a sewage treatment plant and its receiving lake

Cited by 6 publications

References 0 publications

Aerobic Denitrifying Bacteria That Produce Low Levels of Nitrous Oxide

Aerobic Denitrifying Bacteria That Produce Low Levels of Nitrous Oxide

Survival of enterococci and Tn916-like conjugative transposons in soil

Performance Analysis and Microbial Community Evolution of In Situ Biological Biogas Upgrading with Increasing H2/CO2 Ratio

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