Genetic diversity and population genetic analysis of bovine MHC class II <i>DRB3.2</i> locus in three <i>Bos indicus</i> cattle breeds of Southern India

Das, Debanand; Hari, V.; Hatkar, D. N.; Rengarajan, K; Saravanan, R.; Suryanarayana, V. V. S.; Murthy, L. K.

doi:10.1111/j.1744-313x.2012.01126.x

Cited by 13 publications

(8 citation statements)

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“…However, scanty literature is available on the effect of experimental FMD infection on blood and serum profile of cattle, which is important for formulating the ameliorative measures to reduce the biotic stress on the animal due to the infection. In addition, an Indian native breed, Malnad Gidda, is believed to be tolerant of many infectious diseases including FMD; however, no systematic study has been carried out to establish the exactness of this perception [6,7].…”

Section: Introductionmentioning

confidence: 99%

Hematological and serum biochemical profile in cattle experimentally infected with foot-and-mouth disease virus

et al. 2020

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Background and Aim: Foot-and-mouth disease (FMD) is an acute viral infection affecting cloven-hoofed animals causing vesicular erosions in the oral cavity and interdigital space. The present study was undertaken to ascertain the time-dependent changes in clinical, hematological, and biochemical profiles in different breeds of cattle following experimental infection. Materials and Methods: The animals were inoculated with 1.0×104 50% bovine tongue infectious dose (BTID50) by intradermolingual route. Clinical signs were observed, and blood/serum samples were collected at different time intervals. Results: The white blood cell count declined sharply on days 7-13 and recovered on day 14 post-FMD infection. Biochemical analysis of serum markers for vital organ profile revealed no marked damage. However, a significant increase in blood urea nitrogen (BUN) value indicated pre-renal azotemia. Transient hyperthyroidism was indicated by the rise in T3 and T4 that can be correlated with a decrease in triglyceride and total cholesterol levels. In the cardiac damage assessment study, a distinct breed difference was observed wherein Malnad Gidda calves showed no cardiac damage. Conclusion: Except thyroid profile, BUN, and creatine kinase-myocardial band, all other serum biochemical parameters showed no significant abnormalities, whereas lymphopenia is the only hematological change and it is suggested that effective ameliorative measures should be targeted mainly on the feed/water intake, thyroid gland, and the level of lymphocytes.

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Section: Introductionmentioning

confidence: 99%

Hematological and serum biochemical profile in cattle experimentally infected with foot-and-mouth disease virus

et al. 2020

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“…However, according to Lützelschwab et al 13 , Mycobacterium bovis co-infection does not influence the ability of cattle, either they carry or do not, to have the resistant allele in BoLA-DRB3 to bovine leukemia virus. Das et al 14 also documented a highly polymorphic BoLA-DRB3 on exon2 with significant breed-specific genetic diversities in three Bos indicus cattle breeds of South Indian hence recommended conservation to maintain native cattle genetic diversity. In Latin American Creole cattle breeds, Giovambattista et al 15 reported high genetic diversity of MHC DRB3 which could be as a result of multiple sources of germplasm and could be maintained by balancing selection.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Bovine Major Histocompatibility Complex Class II DRB3 locus in cattle breeds from Asia compared to those from Africa and America

et al. 2018

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Genetic polymorphisms and diversity of BoLA-DRB3.2 are essential because of DRB3 gene's function in innate immunity and its association with infectious diseases resistance or tolerance in cattle. The present study was aimed at assessing the level of genetic diversity of DRB3 in the exon 2 (BoLA-DRB3.2) region in African, American and Asian cattle breeds. Amplification of exon 2 in 174 cattle revealed 15 haplotypes. The breeds with the highest number of haplotypes were Brangus (10), Sokoto Gudali (10) and Dajal (9), while the lowest number of haplotypes were found in Holstein and Sahiwal with 4 haplotypes each. Medium Joining network obtained from haplotypic data showed that all haplotypes condensed around a centric area and each sequence (except in H-3, H-51 and H-106) representing almost a specific haplotype. The BoLA-DRB3.2 sequence analyses revealed a non-significant higher rate of non-synonymous (dN) compared to synonymous substitutions (dS). The ratio of dN/dS substitution across the breeds were observed to be greater than one suggesting that variation at the antigen-binding sites is under positive selection; thus increasing the chances of these breeds to respond to wide array of pathogenic attacks. An analysis of molecular variance revealed that 94.01 and 5.99% of the genetic variation was attributable to differences within and among populations, respectively. Generally, results obtained suggest that within breed genetic variation across breeds is higher than between breeds. This genetic information will be important for investigating the relationship between BoLADRB3.2 and diseases in various cattle breeds studied with attendant implication on designing breeding programs that will aim at selecting individual cattle that carry resistant alleles.

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“…According to the data of the review (Behl et al, 2012), among the breeds of species Bos taurus, 20 to 30 alleles are most often found. High allele diversity according to BoLA-DRB3 gene was determined for the Indian breed Malnad Gidda -37 variants (Das et al, 2012), Kalmyk cattle -36 variants, Russian Yaroslavl cattle (Sulimova, 2006) and Mongolian cattle (Ruzina et al, 2010) -35 variants in each, (Ruzina et al, 2010) and Iranian zebu-like Sistani cattle -32 variants (Mohammadi et al, 2009). The recent surveys of local and autochthonous breeds indicate that their allele polymorphism of the BoLA-DRB3 gene is broader than in the commercial and native ones.…”

Section: Discussionmentioning

confidence: 98%

Genetic variability and biodiversity of Ukrainian Gray cattle by the BoLA-DRB3 gene

Suprovych¹,

Suprovych²,

Mokhnachova³

et al. 2021

Regul. Mech. Biosyst.

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At the current stage of genetic studies of cattle, more and more attention is being drawn to autochthonous breeds. Native cattle have a number of prominent phenotypic traits and have preserved unique genes and their combinations lost by modern commercial breeds, which would be valuable to use in selective programs. We surveyed polymorphism of the Ukrainian autochthonous Gray breed according to alleles of exon 2 of the BoLA-DRB3 gene. The uniqueness of the gene lies in the broad variability of its allele variants. Significant informativeness at DNA level is quite important for genetic studies. We surveyed allele polymorphism using the PCR-RLFP method on DNA isolated from 88 samples of blood of cows and 5 samples of sperm. We identified 28 alleles, of which 23 variants were nomenclature ones and 5 (jba, *jab, *jbb, *nad and *nda) were “without established nomenclature”, their share accounting for 8.9%. Four alleles *06, *12, *16 and *jba had a frequency above 5% and occupied 69.9% of the breed’s allele fund overall. The commonest allele was BoLA-DRB3.2*16 (44.1%). In total, we found 40 genotypes. Considering the significant dominance of variant *16, as expected, 5 genotypes with its inclusion occurred: *16/*16, *12/*16, *06/*16, *16/*24 and *jba/*16. It was present in the genotype of two out three studied animals. Parameters of heterozygosity, effective number of alleles, Shannon and Pielou indices indicate that Ukrainian Gray cattle are characterized by lowest level of genetic variability and biodiversity according to the BoLA-DRB3 gene compared with other breeds. Due to significant dominance of allele *16, the breed has no inbred motifs. We noted deviation toward increase in homozygosity without deviations from the norm of the distribution according to Hardy-Weinberg equilibrium. The obtained results will be used for genetic-populational programs with the purpose of improving the genetic potential of cattle breeds in terms of economically beneficial traits and diseases of cattle.

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Genetic diversity and population genetic analysis of bovine MHC class II DRB3.2 locus in three Bos indicus cattle breeds of Southern India

Cited by 13 publications

References 53 publications

Hematological and serum biochemical profile in cattle experimentally infected with foot-and-mouth disease virus

Hematological and serum biochemical profile in cattle experimentally infected with foot-and-mouth disease virus

Genetic Diversity of Bovine Major Histocompatibility Complex Class II DRB3 locus in cattle breeds from Asia compared to those from Africa and America

Genetic variability and biodiversity of Ukrainian Gray cattle by the BoLA-DRB3 gene

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