Genetic diversity and population structure of Saccharomyces cerevisiae isolated from Turkish sourdough by iPBS-retrotransposons markers

Aydin, Furkan; Özer, Göksel; Alkan, Mehtap; Çakır, İbrahim

doi:10.1007/s00203-022-03313-x

Cited by 3 publications

(1 citation statement)

References 51 publications

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“…Intraspecies level polymorphism determined by İPBS markers was also found between isolates of Fusarium oxysporum f. sp. cumini (Özer & Bayraktar, 2018), Saccharomyces cerevisiae (Aydın et al, 2022b) and Rhizoctonia spp. (Erper et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Determination of vc and mating types of Cryphonectria parasitica isolates by multiplex PCR and their genetic diversity in 13 chestnut‐growing provinces of Turkey

et al. 2023

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Vegetative compatibility (vc) and mating types and genetic diversity of Cryphonectria parasitica isolates were determined using 183 isolates obtained from 215 infected chestnut trees growing in 13 provinces of Turkey. Based on the cultural aspects, 143 of these isolates were evaluated as virulent whereas the remaining 40 isolates were hypovirulent. When vc types of 183 isolates were classically differentiated, 135 of them matched to EU‐1 (82.3%), 29 of them to EU‐12 (17.6%) vc type, whereas 19 of them did not match to the two. When molecular vic markers were used, all the isolates were assigned to two EU vc types; 149 to EU‐1 (81.4%) and 34 (18.5%) to EU‐12. Of the majority of the isolates, 134 (73.2%) had mating‐type MAT‐1, while 44 (24%) isolates had MAT‐2 and 5 (2.8%) isolates had both mating types. The population analysis based on two DNA marker systems, Inter‐Primer Binding Site and Start Codon Targeted Polymorphism, showed no intraspecific genetic variation among the C. parasitica isolates. The prevalence of two dominant vc types revealed by this study shows that biological control with hypovirulent EU‐1 and EU‐12 isolates will be significant for the country. The results might be helpful to chestnut breeders carrying out resistance breeding studies to manage this disease based on hypovirulence attributed to Cryphonectria hypovirus 1.

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Section: Discussionmentioning

confidence: 99%

Determination of vc and mating types of Cryphonectria parasitica isolates by multiplex PCR and their genetic diversity in 13 chestnut‐growing provinces of Turkey

et al. 2023

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Isolation, Molecular Characterization, and Population Analysis of Saccharomyces Cerevisiae From Sourdough Collected From Different Provinces

KOÇAK,

ARDIÇ

2024

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Bu çalışmada 3 farklı ilden (Aksaray, Niğde ve Konya) toplanan 18 adet geleneksel ekşi hamur örneğinden Saccharomyces cerevisiae suşlarının izolasyonu, farklı DNA markörleri ile genotipik karakterizasyonu ve popülasyon analizlerinin gerçekleştirilmesi amaçlanmıştır. İzole edilen 72 adet endojen mayanın 58 tanesi S. cerevisiae olarak tanımlanmıştır. Tür içi genetik varyasyonun belirlenmesinde SCoT 13 primeri, iPBS ve ISSR primerlerine göre daha faydalı sonuçlar vermiştir. Popülasyonlar arasındaki mesafe arttıkça genetik uzaklık dereceleri de artış göstermiştir (R=0.74). Popülasyonlar arası (%16) ve popülasyonlar içindeki (%84) genetik varyasyon dereceleri istatistiki olarak önemli bulunmuştur (P < 0.001). UPGMA dendrogramı üzerinde suşlar birçok alt gruba ayrılmış olup STRUCTURE analizine göre anlamlı alt grup sayısı iki çıkmıştır (ΔK=2). UPGMA ve PCoA'ya göre kümelenme suşların izole edildiği bölgelere göre gerçekleşmemiş olup rastgele dağılım gözlemlenmiştir.

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Molecular and Technological Characterization of Saccharomyces cerevisiae from Sourdough

et al. 2023

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DNA markers help detect the intraspecific genetic diversity of yeast strains. Eight ISSR (Inter Simple Sequence Repeats) primers were used to assess the intraspecific diversity of Saccharomyces cerevisiae (n = 96) from different populations (n = 3), evaluate the technological characteristics, and investigate trait-loci associations. The primers amplified 154 reproducible and scorable bands, of which 79.87% were polymorphic. The UPGMA (unweighted pair group method with arithmetic mean) dendrogram clustered 96 isolates into two main clusters, supported by STRUCTURE HARVESTER results (ΔK = 2). Analysis of molecular variance (AMOVA) indicated significant genetic differences between (15%) and within the populations (85%) (p < 0.001). Twenty-nine genetically distinct strains were selected for the technological characterization. Principal component analysis (PCA) revealed that five strains with high fermentation capacity, leavening activity, high growth index at 37 °C, and harsh growth conditions were technologically relevant. Trait-loci association analyses indicated that the highest correlation (r = 0.60) was recorded for the fermentation capacity on the 8th and 113th loci, amplified by ISSR-1 and ISSR-6 primers, respectively (p < 0.05). The strains yielding high performances and the associated loci amplified by ISSR markers possess a high potential to generate locus-specific primers to target the strains with high fermentation capacity.

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Genetic diversity and population structure of Saccharomyces cerevisiae isolated from Turkish sourdough by iPBS-retrotransposons markers

Cited by 3 publications

References 51 publications

Determination of vc and mating types of Cryphonectria parasitica isolates by multiplex PCR and their genetic diversity in 13 chestnut‐growing provinces of Turkey

Determination of vc and mating types of Cryphonectria parasitica isolates by multiplex PCR and their genetic diversity in 13 chestnut‐growing provinces of Turkey

Isolation, Molecular Characterization, and Population Analysis of Saccharomyces Cerevisiae From Sourdough Collected From Different Provinces

Molecular and Technological Characterization of Saccharomyces cerevisiae from Sourdough

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