Genetic diversity and population structure of Indian willow (Salix tetrasperma Roxb.) along its distributional range in the Himalayan region

Sharma, J.P.; Sankhyan, H. P.; Thakur, Sanjeev; Jha, Suman Kumar; Sharma, Rajnish; Poonam,; Gautam, Vikrant

doi:10.1016/j.egg.2021.100096

Cited by 3 publications

(2 citation statements)

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“…These results reflect the outcrossing nature and high gene flow among genotypes. The genetic diversity patterns with moderate gene flow obtained in the current study were similar to those obtained with codominant markers (Sharma et al 2021) suggesting the use of both types of markers for studying genetic variation in this species. The present study has generated valuable information for tree breeders to exploit natural populations of S. tetrasperma and will prove useful for framing new conservation and breeding strategies and their effective implementation in future improvement programmes.…”

Section: Resultssupporting

confidence: 80%

“…In a combined analysis of RAPD and ISSR marker types, AMOVA (Table 4) revealed more genetic variation (87%) within population than among population (13%) due to general characteristics of trees and shrubs (Hamrick et al 1992). The within population variation was recorded higher than the among population variation with SSR markers (Sharma et al 2021). This has also been verified in case of other tree species (Sulima et al 2018;Srihari et al 2013;Sandeep et al 2020) and is in line with the earlier studies which have confirmed that the high rates of out-crossing, life history and seed distribution over a wide range of area may be responsible for possession of genetic variations by woody perennial species majorly within the populations (Hamrick et al 1992;Li et al 2008;Khadivi-Khub et al 2015).…”

Section: Resultsmentioning

confidence: 97%

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Genetic diversity and population structure of Indian willow (Salix tetrasperma Roxb.) with dominant molecular markers along its distribution range in Himalayan region

Sharma¹,

Thakur²,

Sankhyan³

et al. 2022

IJGPB

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Indian willow (Salix tetrasperma) is an agriculturally useful tree which occurs over a wide geographic area across South Asia and bears importance. So far, this species has never been studied for molecular genetic diversity. The present study was, therefore, carried out to assess the genetic diversity and population structure analysis using RAPD and ISSR molecular markers in diverse genotypes from five populations covering North India. The mean number of effective alleles, Shannon information index and gene diversity i.e., 1.38 ± 0.013, 0.35 ± 0.010 and 0.23 ± 0.007, respectively were obtained with RAPD + ISSR markers. The analysis of molecular variance generated by RAPD + ISSR revealed a higher genetic variation (87%) within population as compared to that of among population (13%). Nei genetic distance was maximum (0.185) between Jammu and Kashmir and Punjab populations. Significant Mantel correlation (r=0.551,) was obtained between RAPD and ISSR markers. Bayesian clustering pattern obtained through STRUCTURE software showed four gene pools. Based on the genetic information obtained with regards to Indian willow by combining the RAPD and ISSR marker systems, it is proposed that an individual tree be selected within populations rather than among populations for the improvement of economic traits of Indian willow alongwith conservation of entire ecological populations.

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Section: Resultssupporting

confidence: 80%