Genetic diversity and population structure of the threatened temperate woody bamboo Kuruna debilis (Poaceae: Bambusoideae: Arundinarieae) from Sri Lanka based on microsatellite analysis

Attigala, Lakshmi; Gallaher, Timothy J.; Nason, John D.; Clark, Lynn G.

doi:10.4038/jnsfsr.v45i1.8038

Cited by 11 publications

(5 citation statements)

References 6 publications

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“…The availability of microsatellite markers in bamboo is limited and few genomic SSR markers have been developed for bamboo in recent years (Nayak and Rout 2005;Kaneko et al 2007;Kitamura et al 2009;Dong et al 2012;Dong and Yang 2014). However, a number of researches have highlighted the ability of cross amplification of SSRs in various bamboo species (Sharma et al 2009;Abreu et al 2011;Dong et al 2011;Ndiaye et al 2013;Bhandawat et al 2014;Lin et al 2014;Attigala et al 2017).…”

Section: Genetic Diversitymentioning

confidence: 99%

Genetic diversity and structure of Dendrocalamus hamiltonii natural metapopulation: a commercially important bamboo species of northeast Himalayas

et al. 2019

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Dendrocalamus hamiltonii is a commercially important bamboo species of India, experiencing population depletion due to heavy extraction from natural forests. Nuclear simple sequence repeats (nSSRs) were used to study the genetic diversity and population genetic structure of 19 natural stands of D. hamiltonii distributed across the northeast Himalayas. A total of 68 nSSR primer pairs of D. latiflorus and Bambusa arundinacea have been tested in D. hamiltonii for their transferability, out of which 17 primers showing positive and polymorphic amplification were used for genotyping. A total of 130 alleles were generated in 535 individuals of all the populations using selected primer pairs. The marker analysis indicated that D. hamiltonii populations have maintained a low level of genetic diversity (h = 0.175, I = 0.291) in northeastern region of India. Despite a large proportion of the genetic variation (83.47%) confined within the populations, a moderate level of genetic differentiation (F ST = 0.165) was observed among the populations. The clustering pattern obtained in UPGMA and STRU CTU RE analysis revealed that most of the populations were clustered in accordance with their geographical distribution. Two populations (DH03 and DH13) exhibiting significant genetic admixture were identified and recommended for in situ conservation. In addition, six highly diverse populations were also suggested for conservation in different geographical area under study. The study has revealed useful nSSR markers for D. hamiltonii, which were lacking earlier and the information generated herein is of paramount importance in devising programs for species conservation and genetic improvement.

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Section: Genetic Diversitymentioning

confidence: 99%

Genetic diversity and structure of Dendrocalamus hamiltonii natural metapopulation: a commercially important bamboo species of northeast Himalayas

et al. 2019

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“…& L.G. Clark (Attigala et al, 2017) and that ( He = 0.541) for 18 populations of Dendrocalamus sinicus L.C. Chia & J.L.…”

Section: Discussionmentioning

confidence: 95%

Population genetic structure of the giant panda staple food bamboo (Fargesia spathacea complex) and its taxonomic implications

Huang

Xing

et al. 2020

J of Sytematics Evolution

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The taxonomy of woody bamboo presents many difficulties due to its long blooming interval and complex morphological variation. Whether the current taxonomy reflects genuine species divergence within woody bamboo is an intriguing question. The Fargesia spathacea Franch. complex comprises 15 closely related species with a sympatric distribution in China. Their classification has long been controversial because only a handful of vegetative traits are available, providing a good opportunity to explore the evolutionary relationships and genetic differentiation in woody bamboo. Here, we present a study involving 750 individuals from 39 representative populations in the F. spathacea complex using 14 simple sequence repeat markers. We found varying degrees of genetic diversity across populations of the F. spathacea complex (He = 0.07–0.81) and largely negative F‐values at the population level, implying an excess of heterozygotes in the populations. Phylogenetic analyses revealed that all populations were divided into two major groups (clusters A and B), with the majority of the 15 species representing distinct genetic lineages. Based on population genetic analysis along with morphological evidence, we confirmed the identity of three species (F. decurvata J. L. Lu, F. spathacea, and F. murielae Gamble) and suggested the invalidation of four other species (F. scabrida T. P. Yi, F. robusta T. P. Yi, F. denudata T. P. Yi F. murielae (Gamble) T. P. Yi, and F. nitida (Mitford) Keng f. ex T. P. Yi). The delimitation of the remaining eight species has yet to be explored. The analysis of ecological factors and spatial autocorrelation suggested that altitudinal differences might account for the distinct genetic divergence between the two major groups.

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“…'s study (2012) on D. membranaceus using 10 ISSR markers also reported an AMOVA result of 78.95% within the population and 21.05% among populations. Again, Attigala, Gallaher, Nason, and Clark (2017) on the study of genetic diversity and population structure of the threatened temperate woody bamboo Kuruna debilis (Poaceae: Bambusoideae: Arundinarieae) from Sri Lanka using 12 microsatellite loci reported an AMOVA result of 8.35% among groups, 7.52% among populations within groups, and 84.13% within populations. But compared to the above work on different bamboo species, the within‐population percentage of 61.05% for O. abyssinica is smaller.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity, population structure, and gene flow analysis of lowland bamboo [Oxytenanthera abyssinica (A. Rich.) Munro] in Ethiopia

Oumer

Dagne

Feyissa

et al. 2020

Ecology and Evolution

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Bamboo, a member of subfamily Bambusoideae in the grass family (Poaceae), is one of the most important nontimber forest resources and a potential alternative to wood and wood products. Ethiopian lowland bamboo ( Oxytenanthera abyssinica ) is an economically and ecologically important species which accounts about 85% of total bamboo coverage in the country. This species is experiencing population decline due to a number of anthropogenic factors. As a foundation step, genetic diversity, population structure, and gene flow analysis of various O. abyssinica populations found in Ethiopia are studied using inter‐simple sequence repeat markers. One hundred and thirty isolates of bamboo belonging to 13 geographically diverse populations were collected for DNA extraction and analysis. Heterozygosity, level of polymorphism, marker efficiency, Nei's gene diversity ( H ), and Shannon's information index ( I ) analysis, analysis of molecular variance (AMOVA), analysis for cluster, principal coordinates (PCoA), and admixture analyses were performed based on the markers banding pattern. The results indicated high genetic variation (84.48%) at species level. The H , I , observed and effective number of alleles at the species level were 0.2702, 0.4061, 1.8448, and 1.4744, respectively, suggesting a relatively high level of genetic diversity. However, genetic differentiation at the population level was relatively low. Using grouped populations, AMOVA revealed that most (61.05%) of the diversity was distributed within the populations with F ST = 0.38949, F SC = 0.10486, and F CT = 0.31797. Cluster analysis grouped the populations into markedly distinct clusters, suggesting confined propagation in distinct geographic regions. STRUCTURE analyses showed K = 2 for all populations and K = 11 excluding Gambella population. Using these markers, we found strong evidence that the genetic diversity of the lowland bamboo is associated with distinct geographic regions and that isolates of Gambella Region, with their unique genetic origin, are quite different from other bamboos found in the country.

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Genetic diversity and population structure of the threatened temperate woody bamboo Kuruna debilis (Poaceae: Bambusoideae: Arundinarieae) from Sri Lanka based on microsatellite analysis

Cited by 11 publications

References 6 publications

Genetic diversity and structure of Dendrocalamus hamiltonii natural metapopulation: a commercially important bamboo species of northeast Himalayas

Genetic diversity and structure of Dendrocalamus hamiltonii natural metapopulation: a commercially important bamboo species of northeast Himalayas

Population genetic structure of the giant panda staple food bamboo (Fargesia spathacea complex) and its taxonomic implications

Genetic diversity, population structure, and gene flow analysis of lowland bamboo [Oxytenanthera abyssinica (A. Rich.) Munro] in Ethiopia

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