Genetic diversity and population structure of the endemic Azorean juniper, Juniperus brevifolia (Seub.) Antoine, inferred from SSRs and ISSR markers

Bettencourt, Sílvia; Mendonça, Duarte; Lopes, M. S.; Rocha, Sara; Monjardino, P.; Monteiro, Lisandra; Machado, Artur da Câmara

doi:10.1016/j.bse.2015.02.003

Cited by 12 publications

(17 citation statements)

References 45 publications

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“…The number of migrants per generation was very high (N m =9.92) indicating a continuous gene flow in J. communis populations in the past. A particular wind could transport pollen over long distances and, therefore, promote high N m (Bettencourt et al 2015). Slatkin (1987) and Ferreira and Eriksson (2006) determined that more than four migrants per generation prevent genetic differentiation between populations.…”

Section: Genetic Diversity and Differentiationmentioning

confidence: 99%

Genetic structure and diversity in Juniperus communis populations in Saxony, Germany

Reim¹,

Lochschmidt²,

Proft³

et al. 2016

Biodiversity Research and Conservation

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In recent years, land use changes led to a rapid decline and fragmentation of J. communis populations in Germany. Population isolation may lead to a restricted gene flow and, further, to negative effects on genetic variation. In this study, genetic diversity and population structure in seven fragmented J. communis populations in Saxony, Germany, were investigated using nuclear microsatellites (nSSR) and chloroplast single nucleotide polymorphism (cpSNP). In all Saxony J. communis populations, a high genetic diversity was determined but no population differentiation could be detected whatever method was applied (Bayesian cluster analysis, F-statistics, AMOVA). The same was true for three J. communis out-group samples originating from Italy, Slovakia and Norway, which also showed high genetic diversity and low genetic differences regarding other J. communis populations. Low genetic differentiation among the J. communis populations ascertained with nuclear and chloroplast markers indicated high levels of gene flow by pollen and also by seeds between the sampled locations. Low genetic differentiation may also provide an indicator of Juniper survival during the last glacial maximum (LGM) in Europe. The results of this study serve as a basis for the implementation of appropriate conservation measures in Saxony.

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Section: Genetic Diversity and Differentiationmentioning

confidence: 99%

Genetic structure and diversity in Juniperus communis populations in Saxony, Germany

Reim¹,

Lochschmidt²,

Proft³

et al. 2016

Biodiversity Research and Conservation

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show abstract

“…; Sanchez-Gomez et al, 2013). Distinct ISSR primer sets applied in different investigations might also be one of the reasons in result bias: all seven primers used for J. brevifolia by Silva et al (2011) were the same as in our examination, while only three primers corresponded to the ones used in J. brevifolia survey (Bettencourt et al, 2015). Both (RAPD and ISSR) dominant markers showed rich allelic diversity in J. brevifolia accessions and a moderate genetic similarity (Lima et al, 2010).…”

Section: Discussionmentioning

confidence: 67%

“…For Lithuanian populations of J. communis Mantel test showed significant moderate correlation between geographic and ISSR-based genetic distances and all individuals were correctly allocated to their populations by cluster analysis. ISSR data of J. brevifolia showed no statistically significant genetic structure by geographic distance (Bettencourt et al, 2015), while low correlations were detected comparing ISSR and geography variables for the other conifer genera (Sanchez-Gomez et al, 2013).…”

Section: Discussionmentioning

confidence: 79%

“…It is in agreement to 45% among population variability documented for endemic populations of J. brevifolia from the Azorean archipelago (Silva et al, 2011), where different habitat populations were compared (lowland coastal populations displayed a significantly higher diversity than the mountain populations). In the surveys where habitat types were not mentioned for selected sites, interpopulation variability at ISSR loci for populations was lower: for J. phoenica -12% (Meloni et al, 2006) and for J. brevifolia 7% (Bettencourt et al, 2015). Direct comparison of separate studies is difficult due to numerous factors, influencing genetic diversity (species breeding system, geographical range, etc.…”

Section: Discussionmentioning

confidence: 99%

“…Caryocedrus, were compared by ISSRs (Adams et al, 2003). A few studies were devoted for evaluation of molecular diversity within juniper species: ISSR markers were applied for J. brevifolia (Lima et al, 2010;Silva et al, 2011;Bettencourt et al, 2015) and J. phoenicea populations (Meloni et al, 2006). Prior to our study, the only available information about J. communis obtained by RAPD markers came from Scotland (Adams, 2011) and Iran (Kasaian et al, 2011), while ISSR data for this species are still missing.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of Lithuanian populations of Juniperus communis L. in relation to abiotic and biotic factors

et al. 2016

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Juniperus communis L. is increasingly threatened in many parts of Europe, including the Baltic region. Our present study was aimed at evaluation of genetic diversity of J. communis populations of Lithuania. Fourteen selected populations differed in geography and habitats (coastal brown dunes covered with natural Scots pine forests, further referred as B; J. communis shrubs, F; transition mires and quaking bogs, D; subcontinental moss Scots pine forests, G; xero-thermophile fringes, E). Molecular variance was analyzed among populations (140 individuals, in total), employing 14 Inter Simple Sequence Repeat (ISSR) markers. Percentage of polymorphic loci differed between populations belonging to the different habitat type, being the highest for population representing habitat F (68.2%) and the lowest for populations D (42.9%). The data demonstrate the possibility to underestimate or overestimate this parameter if some habitat populations are bypassed. Means per population of Nei's gene diversity and Shannon's information indexes, were 0.158 and 0.239, respectively. Significant correlation between genetic and geographic distance of populations of J. communis was documented by Mantel test. Bayesian analysis of ISSR data has separated populations of Southern Lithuania from Northern part. Juniperus communis populations representing different habitats showed moderate interpopulation variance at ISSR loci. In ISSR-based dendrograms, individuals were correctly allocated to populations, even in case of populations growing in 0.5 km vicinity (habitats D and G). Among all populations the most distinct one was representing habitat of J. communis shrubs (F) protected by EUNIS. Principal coordinate analysis of weighted averages of Ellenberg's indicator values (EIV) for herbaceous species, separated populations according to habitat type, while the same type analysis of ISSR data allowed distinguishing E habitat populations from populations of all other habitat type but G. Our study shows that on the local (Lithuanian) scale, molecular variance between populations of J. communis at ISSR loci might be related to habitat type. For evaluation and preservation of diversity of J. communis, different assessment methods should be included and management strategies should be directed in the way to retain variety of habitats encompassing both widely spread and less common ones.

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Late-Weichselian (Vistulian) environmental changes in NE Poland – Evidence from Lake Suchar Wielki

Fiłoc,

Kupryjanowicz,

Suchora

et al. 2024

CATENA

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Genetic diversity and population structure of the endemic Azorean juniper, Juniperus brevifolia (Seub.) Antoine, inferred from SSRs and ISSR markers

Cited by 12 publications

References 45 publications

Genetic structure and diversity in Juniperus communis populations in Saxony, Germany

Genetic structure and diversity in Juniperus communis populations in Saxony, Germany

Genetic diversity of Lithuanian populations of Juniperus communis L. in relation to abiotic and biotic factors

Late-Weichselian (Vistulian) environmental changes in NE Poland – Evidence from Lake Suchar Wielki

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