Genetic Diversity and Population Structure of Toona Ciliata Roem. Based on Sequence-Related Amplified Polymorphism (SRAP) Markers

Abstract

Sequence-related amplified polymorphism (SRAP) markers were used to investigate the genetic diversity among 30 populations of Toona ciliata Roem. sampled from the species' distribution area in China. To analyze the polymorphism in the SRAP profiles, 1505 primer pairs were screened and 24 selected. A total of 656 SRAP bands ranging from 100 to 1500 bp were acquired, of these 505 bands (77%) were polymorphic. The polymorphism information content (PIC) values ranged from 0.32 to 0.45, with an average of 0.41. An analysis of molecular variance (AMOVA) indicated that the most significant variation was attributable to differences among the populations and that variation within the populations was OPEN ACCESSForests 2015, 6 1095 small. STRUCTURE analysis divided the 30 populations into two parts. The unweighted pair group method of arithmetic averages (UPGMA) clustering and principal coordinates analysis (PCoA) showed that the 30 populations could be classified into four types. The results demonstrate a clear geographical trend for T. ciliata in China and provide a theoretical basis for future breeding and conservation strategy of T. ciliata.

Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers

“…This level of variation was corroborated by other variables such as percentage of polymorphic loci (PPL), ranging 18.2-50% with an average of 30.5%. The PPL value in this study was higher than previous RAPD studies of T. sinensis in West Java (Hidayat and Siregar 2011) and Toona ciliata in China (Li et al 2015), but lower than one of T. sinensis in China (Wang et al 2008).…”

“…Other genetic parameter being measured was value of expected heterozygosity (He) with the value of Hewas 0.304. This He value in this study was similar to those of T. ciliata in China (He 0.305 in Liu et al (2014); He 0.378 in Li et al (2015)), T. sinensis in West Java (He 0.240) and T. sinensis in China (He 0,333 in Wang et al (2008)). …”

“…In general, RAPD can provide valuable data about genetic variations within and among populations of a species, given that data (Collignon et al 2002). This technique has been applied for detecting population genetic diversity of Toona sinensis (Wang et al 2008;Hidayat and Siregar 2011), Toona australis (Tavares et al 2012), Toona ciliata (Liu et al 2014;Li et al 2015), Melia azedarach (Yulianti 2011), Melia volkeensii (Runo et al 2004), Lathyrus sativus (Nosrati et al 2012), Gmelina arborea (Wee et al 2012), Quercus sp. (Dering and Chybicki 2012), Pinus nigra (Çengel et al 2012), and Lansium domesticum (Song 2000).…”

An integrating study of genetic diversity and ecological niche modelling in Salvia aristata (Lamiaceae)

“…Sequence-related amplified polymorphism (SRAP) is a marker which amplifies open reading frames (ORFs) with specific forward and reverse primers. Compared with the other dominant markers, simplicity, reproducibility, cost effectiveness and high throughput of SRAP make it more applicable in the genomic studies (Chang et al 2012, Li and Quiros 2001, Li et al 2015, Robarts and Wolfe 2014. According to the previous studies, SRAP marker is very successful for detecting inter-and intra-population genetic variations (Erbano et al 2015, Robarts and Wolfe 2014, Talebi et al 2015.…”

Population Structure and Genetic Relationships of Melia Taxa in China Assayed with Sequence-Related Amplified Polymorphism (SRAP) Markers

“…In comparison with other species in the same family (Meliaceae), Melia had a degree of population differentiation comparable to those in Swietania macrophylla King, Toona ciliate Roemer, and Chukrasia [46][47][48], suggesting a similar reproductive ecology among different genera in Meliaceae. Furthermore, analogous to the genus Melia, the genus Chukrasia had two morphologically distinct groups of populations.…”